A simple rapid detection method of DNA based on ligation-mediated real-time fluorescence PCR

The Analyst ◽  
2013 ◽  
Vol 138 (19) ◽  
pp. 5745 ◽  
Author(s):  
Yu Qing Du ◽  
Peng Fei Gao ◽  
Wei Wang ◽  
Ting Ting Wang ◽  
Yong Chang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Detection Method

The Most Rapid Detection Method against Korean Sacbrood Virus using Ultra-Rapid Reverse-Transcription Real-Time PCR (URRTRT-PCR)

2016 ◽  
Vol 31 (2) ◽  
pp. 121
Author(s):  
Sang-Hyun Min ◽  
Jee-Hee Wang ◽  
Su-Jin Lim ◽  
Chil-Woo Lee ◽  
Byoung-Su Yoon
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Detection Method ◽  
Sacbrood Virus

Simultaneous and rapid detection method for measles and rubella using single-tube multiplex real-time quantitative RT-PCR

2019 ◽  
Vol 25 (10) ◽  
pp. 829-831 ◽  
Author(s):  
Nori Yoshioka ◽  
Hideharu Hagiya ◽  
Matsuo Deguchi ◽  
Shigeto Hamaguchi ◽  
Masanori Kagita ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Detection Method ◽  
Rt Pcr ◽  
Single Tube

Establishment of rapid detection method and surveillance of budgerigar fledgling disease virus using a TaqMan Real-Time PCR

2019 ◽  
Vol 43 ◽  
pp. 80-83 ◽  
Author(s):  
Jingjiao Ma ◽  
Ye Tian ◽  
Min Zhang ◽  
Yujie Li ◽  
Weili Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Detection Method

Agricultural Machinery Operation Posture Rapid Detection Method Based on Intelligent Sensor

2013 ◽  
Vol 373-375 ◽  
pp. 936-939
Author(s):  
Nan Feng Zhang ◽  
Jing Feng Yang ◽  
Yue Ju Xue ◽  
Zhong Li ◽  
Xiao Lin Huang
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Inertial Measurement Unit ◽  
Detection Method ◽  
Measurement Unit ◽  
Test Results ◽  
Agricultural Machinery ◽  
Intelligent Sensor ◽  
Inertial Measurement ◽  
Status Data

Based on agricultural machinery body posture detection parameters and wheels gesture detection parameters collected by gyro inertial measurement unit, an agricultural machinery operation posture rapid detection method is proposed in this paper. The test results show that, the test results of the method are accurate and available, and the method is effective and available for real-time body and wheel status data to further understand the agricultural machinery.

Rapid Detection Method for Bacillus anthracis Using a Combination of Multiplexed Real-Time PCR and Pyrosequencing and Its Application for Food Biodefense

2015 ◽  
Vol 78 (2) ◽  
pp. 355-361 ◽  
Author(s):  
TIMOTHY W. JANZEN ◽  
MATTHEW C. THOMAS ◽  
NORIKO GOJI ◽  
MICHAEL J. SHIELDS ◽  
KRISTEN R. HAHN ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Detection Method ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Health Concern ◽  
Sequential Method ◽  
Critical Gap

Bacillus anthracis, the causative agent of anthrax, has the capacity to form highly resilient spores as part of its life cycle. The potential for the dissemination of these spores using food as a vehicle is a huge public health concern and, hence, requires the development of a foodborne bioterrorism response approach. In this work, we address a critical gap in food biodefense by presenting a novel, combined, sequential method involving the use of real-time PCR and pyrosequencing for the rapid, specific detection of B. anthracis spores in three food matrices: milk, apple juice, and bottled water. The food samples were experimentally inoculated with 40 CFU ml−1, and DNA was extracted from the spores and analyzed after immunomagnetic separation. Applying the combination of multiplex real-time PCR and pyrosequencing, we successfully detected the presence of targets on both of the virulence plasmids and the chromosome. The results showed that DNA amplicons generated from a five-target multiplexed real-time PCR detection using biotin-labeled primers can be used for single-plex pyrosequencing detection. The combined use of multiplexed real-time PCR and pyrosequencing is a novel, rapid detection method for B. anthracis from food and provides a tool for accurate, quantitative identification with potential biodefense applications.

scholarly journals Establishment of a rapid detection method for wheat crown rot

2021 ◽  
Vol 251 ◽  
pp. 02027
Author(s):  
Lina Sui ◽  
Jishun Li ◽  
Jindong Hu ◽  
Yan Wang
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Rapid Detection ◽  
Rhizosphere Soil ◽  
Detection Method ◽  
Early Stage ◽  
Fusarium Species ◽  
Crown Rot ◽  
Fusarium Pseudograminearum ◽  
Standard Curve

We have established a real-time fluorescent quantitative PCR system that can detect wheat crown rot rapidly and accurately quantify fungi, Fusarium species and Fusarium pseudograminearum in the rhizosphere soil of infected wheat through the standard curve produced, with a view to the early stage of wheat Provide help in predicting the occurrence of wheat crown rot.

Simple and rapid detection method for the mutations in SLC22A12 that cause hypouricemia by allele-specific real-time polymerase chain reaction

2013 ◽  
Vol 415 ◽  
pp. 330-333 ◽  
Author(s):  
Shota Takagi ◽  
Risa Omae ◽  
Juliet O. Makanga ◽  
Tetsuya Kawahara ◽  
Tetsuya Inazu
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Rapid Detection ◽  
Detection Method ◽  
Chain Reaction ◽  
Allele Specific ◽  
Polymerase Chain

A rapid detection method for policy-sensitive amines real-time supervision

Talanta ◽  
2018 ◽  
Vol 178 ◽  
pp. 636-643 ◽  
Author(s):  
Haixu Zhang ◽  
Jinian Shu ◽  
Bo Yang ◽  
Peng Zhang ◽  
Pengkun Ma
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Detection Method
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