Development of a rapid detection method for real-time fluorescent quantitative PCR of Salmonella spp. and Salmonella Enteritidis in ready-to-eat fruits and vegetables

LWT ◽  
2021 ◽  
pp. 111837
Author(s):  
Jiajia Wan ◽  
Liping Zheng ◽  
Liangyu Kong ◽  
Zhaoxin Lu ◽  
Yang Tao ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Rapid Detection ◽  
Salmonella Enteritidis ◽  
Fruits And Vegetables ◽  
Detection Method ◽  
Salmonella Spp

scholarly journals Establishment of a rapid detection method for wheat crown rot

2021 ◽  
Vol 251 ◽  
pp. 02027
Author(s):  
Lina Sui ◽  
Jishun Li ◽  
Jindong Hu ◽  
Yan Wang
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Rapid Detection ◽  
Rhizosphere Soil ◽  
Detection Method ◽  
Early Stage ◽  
Fusarium Species ◽  
Crown Rot ◽  
Fusarium Pseudograminearum ◽  
Standard Curve

We have established a real-time fluorescent quantitative PCR system that can detect wheat crown rot rapidly and accurately quantify fungi, Fusarium species and Fusarium pseudograminearum in the rhizosphere soil of infected wheat through the standard curve produced, with a view to the early stage of wheat Provide help in predicting the occurrence of wheat crown rot.

A simple rapid detection method of DNA based on ligation-mediated real-time fluorescence PCR

The Analyst ◽  
2013 ◽  
Vol 138 (19) ◽  
pp. 5745 ◽  
Author(s):  
Yu Qing Du ◽  
Peng Fei Gao ◽  
Wei Wang ◽  
Ting Ting Wang ◽  
Yong Chang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Detection Method

scholarly journals Rapid detection and differentiation of Salmonella species, Salmonella Typhimurium and Salmonella Enteritidis by multiplex quantitative PCR

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206316 ◽  
Author(s):  
Raymond Heymans ◽  
Amir Vila ◽  
Caroliene A. M. van Heerwaarden ◽  
Claudia C. C. Jansen ◽  
Greetje A. A. Castelijn ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Quantitative Pcr ◽  
Rapid Detection ◽  
Salmonella Enteritidis

Detection and Enumeration of Salmonella Enteritidis in Homemade Ice Cream Associated with an Outbreak: Comparison of Conventional and Real-Time PCR Methods

2006 ◽  
Vol 69 (3) ◽  
pp. 639-643 ◽  
Author(s):  
K. H. SEO ◽  
I. E. VALENTIN-BON ◽  
R. E. BRACKETT
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Pcr ◽  
Salmonella Enteritidis ◽  
Direct Detection ◽  
Ice Cream ◽  
Plate Count ◽  
Pcr Assay ◽  
The Real ◽  
Real Time Quantitative Pcr

Salmonellosis caused by Salmonella Enteritidis (SE) is a significant cause of foodborne illnesses in the United States. Consumption of undercooked eggs and egg-containing products has been the primary risk factor for the disease. The importance of the bacterial enumeration technique has been enormously stressed because of the quantitative risk analysis of SE in shell eggs. Traditional enumeration methods mainly depend on slow and tedious most-probable-number (MPN) methods. Therefore, specific, sensitive, and rapid methods for SE quantitation are needed to collect sufficient data for risk assessment and food safety policy development. We previously developed a real-time quantitative PCR assay for the direct detection and enumeration of SE and, in this study, applied it to naturally contaminated ice cream samples with and without enrichment. The detection limit of the real-time PCR assay was determined with artificially inoculated ice cream. When applied to the direct detection and quantification of SE in ice cream, the real-time PCR assay was as sensitive as the conventional plate count method in frequency of detection. However, populations of SE derived from real-time quantitative PCR were approximately 1 log higher than provided by MPN and CFU values obtained by conventional culture methods. The detection and enumeration of SE in naturally contaminated ice cream can be completed in 3 h by this real-time PCR method, whereas the cultural enrichment method requires 5 to 7 days. A commercial immunoassay for the specific detection of SE was also included in the study. The real-time PCR assay proved to be a valuable tool that may be useful to the food industry in monitoring its processes to improve product quality and safety.

The Most Rapid Detection Method against Korean Sacbrood Virus using Ultra-Rapid Reverse-Transcription Real-Time PCR (URRTRT-PCR)

2016 ◽  
Vol 31 (2) ◽  
pp. 121
Author(s):  
Sang-Hyun Min ◽  
Jee-Hee Wang ◽  
Su-Jin Lim ◽  
Chil-Woo Lee ◽  
Byoung-Su Yoon
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Detection Method ◽  
Sacbrood Virus

Rapid Detection for Salmonella spp. by Ultrafast Real-time PCR Assay

2018 ◽  
Vol 33 (1) ◽  
pp. 50-57
Author(s):  
Seok Hwan Kim ◽  
Yu-Si Lee ◽  
In-Sun Joo ◽  
Hyo Sun Kwak ◽  
Gyung Tae Chung ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Salmonella Spp

Next day Salmonella spp. detection method based on real-time PCR for meat, dairy and vegetable food products

2014 ◽  
Vol 184 ◽  
pp. 113-120 ◽  
Author(s):  
David Rodriguez-Lazaro ◽  
Patricia Gonzalez-García ◽  
Elisabetta Delibato ◽  
Dario De Medici ◽  
Rosa Maria García-Gimeno ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Detection Method ◽  
Food Products ◽  
Salmonella Spp ◽  
Vegetable Food
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