An accurate and transferable protocol for reproducible quantification of organic pollutants in human serum using direct isotope dilution mass spectrometry

The Analyst ◽  
2014 ◽  
Vol 139 (23) ◽  
pp. 6223-6231 ◽  
Author(s):  
Andrew J. Boggess ◽  
G. M. Mizanur Rahman ◽  
Matt Pamukcu ◽  
Scott Faber ◽  
H. M. Skip Kingston
Keyword(s):  
Mass Spectrometry ◽  
Human Serum ◽  
Organic Pollutants ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Direct Quantification

A novel and transferable method for the accurate and direct quantification of organic pollutants in human serum.

Determination of Cystatin C in human serum by isotope dilution mass spectrometry using mass overlapping peptides

2015 ◽  
Vol 112 ◽  
pp. 141-155 ◽  
Author(s):  
Ana González-Antuña ◽  
Pablo Rodríguez-González ◽  
Rudiger Ohlendorf ◽  
André Henrion ◽  
Vincent Delatour ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Serum ◽  
Cystatin C ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry

Quantification of non-transferrin bound iron (NTBI) in human serum by isotope dilution mass spectrometry (IDMS)

2019 ◽  
Vol 34 (10) ◽  
pp. 1988-1997 ◽  
Author(s):  
Dongxiao Yang ◽  
Xue Qin Ng ◽  
Thomas Walczyk
Keyword(s):  
Mass Spectrometry ◽  
Human Serum ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry

NTA·57Fe is used to capture NTBI and saturate transferrin with iron so that NTBI can be separated through ultrafiltration.

Determination of steroid hormones in a human-serum reference material by isotope dilution--mass spectrometry: a candidate definitive method for cortisol.

1984 ◽  
Vol 30 (5) ◽  
pp. 619-626 ◽  
Author(s):  
D G Patterson ◽  
M B Patterson ◽  
P H Culbreth ◽  
D M Fast ◽  
J S Holler ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Serum ◽  
Isotope Dilution ◽  
Degradation Products ◽  
Trimethylsilyl Ether ◽  
Isotope Dilution Mass Spectrometry ◽  
Linear Interpolation ◽  
Negative Ion ◽  
Gas Chromatography Mass Spectrometry ◽  
Definitive Method

Abstract We report a method, based on isotope dilution--mass spectrometry, for determining cortisol in a pooled specimen of human serum. Isotopically labeled cortisol is added to 5.0 mL of serum so that the molar concentrations of labeled cortisol and unlabeled cortisol are approximately equal. The specimen and two calibration standards are extracted with dichloromethane, and the extracted cortisol is converted to the methoxime-trimethylsilyl ether derivative. Samples and standards are analyzed by gas chromatography--mass spectrometry by monitoring the peak areas for m/z 605 and 608. The cortisol concentration is calculated by linear interpolation between the two bracketing standards. Variances of data collected during six weeks showed that the overall coefficient of variation (CV) was 0.69% (n = 32); the within-vial CV, 0.63%; the among-vial CV, 0.22%; and the among-day CV, 0.15% (means = 3.973 nmol/vial). Method specificity was demonstrated by liquid chromatographic as well as C8 mini-column cleanup of samples before derivation, by alternative ion monitoring at m/z 636 and 639, and by negative-ion chemical ionization at m/z 459 and 462. Derivatives of all observed degradation products of cortisol under basic, neutral, and acidic conditions did not interfere.

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