A novel amino acid analysis method using derivatization of multiple functional groups followed by liquid chromatography/tandem mass spectrometry

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1965-1973 ◽  
Author(s):  
Yohei Sakaguchi ◽  
Tomoya Kinumi ◽  
Taichi Yamazaki ◽  
Akiko Takatsu
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Functional Groups ◽  
Amino Acid Analysis ◽  
Hydroxyl Groups ◽  
Tandem Mass ◽  
Analysis Method ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Phenolic Hydroxyl Groups

We have developed a novel amino acid analysis method using derivatization of multiple functional groups (amino, carboxyl, and phenolic hydroxyl groups).

Considerations for amino acid analysis by liquid chromatography-tandem mass spectrometry: A tutorial review

2020 ◽  
Vol 131 ◽  
pp. 116018
Author(s):  
Jake P. Violi ◽  
David P. Bishop ◽  
Matthew P. Padula ◽  
Joel R. Steele ◽  
Kenneth J. Rodgers
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Tandem Mass Spectrometry ◽  
Amino Acid Analysis ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Rapid comprehensive amino acid analysis by liquid chromatography/tandem mass spectrometry: comparison to cation exchange with post-column ninhydrin detection

2008 ◽  
Vol 22 (22) ◽  
pp. 3481-3488 ◽  
Author(s):  
Dennis J. Dietzen ◽  
Annette L. Weindel ◽  
Mary O Carayannopoulos ◽  
Michael Landt ◽  
Ellen T. Normansell ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Tandem Mass Spectrometry ◽  
Cation Exchange ◽  
Amino Acid Analysis ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Validation of a rapid, comprehensive and clinically relevant amino acid profile by underivatised liquid chromatography tandem mass spectrometry

2020 ◽  
Vol 58 (5) ◽  
pp. 758-768 ◽  
Author(s):  
Rachel S. Carling ◽  
Kate John ◽  
Richard Churchus ◽  
Charles Turner ◽  
R. Neil Dalton
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Amino Acid Profile ◽  
Ion Pair ◽  
Analysis Time ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

AbstractBackgroundQuantification of plasma amino acids is key to the diagnosis of inherited defects of amino acid synthesis, catabolism and transport, many of which present as clinical emergencies. The utility of this test is limited by the long analysis time and subsequent inability of laboratories to provide results in real-time. Traditionally, analysis has been performed by ion exchange chromatography (IEC) but recently there has been a move towards liquid chromatography tandem mass spectrometry (LC-MS/MS) which provides the potential for faster analysis. However, the necessity to derivatise the sample and/or utilise an ion-pair reagent, combined with lack of commercially available stable isotope internal standards (IS) has prevented laboratories fully exploiting the benefits of this methodology. We describe an underivatised LC-MS/MS method enabling patient results to be reported with an improved turnaround time (<1 h).MethodsMethanolic IS was added to plasma (10 μL) to precipitate protein. Following centrifugation amino acids were analysed by LC-MS/MS using selected reaction monitoring (SRM) for each analyte and corresponding IS.ResultsPatient samples (n = 57) and external quality assessment (EQA) material (n = 11) were analysed and results compared with IEC. Comparable accuracy and precision were obtained with 15-min analysis time.ConclusionsThis method enables the analysis of a clinically comprehensive amino acid profile without the need for derivatisation/ion-pair reagents and benefitting from improved analytical quantitation through multipoint calibration and use of stable isotope IS. The analysis time is fast in comparison to IEC, improves efficiency of laboratory workflow and enables stat analysis of clinically urgent samples.

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