A sensitive one-step method for quantitative detection of α-amylase in serum and urine using a personal glucose meter

Abstract We synthesized o-(4,6-o-isopropylidene-alpha-D-glucopyranosyl)-(1----4)- [o-alpha-D-glucopyranosyl-(1----4])5-o-alpha-D-glucopyranosyl-(1----2)- alpha-D-fructofuranoside (IPG7F) and developed an assay for determining the activity of amylase in human serum and urine by using this substrate. Glucoamylase, alpha-glucosidase, and mannitol dehydrogenase are used as coupling enzymes. The coupled reactions are monitored by continuously measuring the oxidation rate of NADH. In this procedure, various substances in the test specimens do not interfere with the detection of amylase activity. Exactly one molecule of NADH is oxidized by one attack of amylase on the substrate, although four products can be produced in the reaction. The within-assay coefficient of variation (CV) ranged from 1.0% to 4.1% and the between-assay CV ranged from 2.6% to 5.3%. The results of our new assay correlate well with those of the amylase assay involving p-nitrophenol maltoheptaoside as substrate (r = 0.978) and with those of the amylase assay involving maltopentaose (r = 0.987).

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A one-step method for the isolation and determination of leaf ribulose-1,5-diphosphate carboxylase

Analytical Biochemistry ◽

10.1016/0003-2697(71)90191-6 ◽

1971 ◽

Vol 41 (1) ◽

pp. 57-66 ◽

Cited By ~ 65

Author(s):

Jonathan J. Goldthwaite ◽

Lawrence Bogorad

Keyword(s):

Step Method ◽

One Step

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Direct determination of aluminium in serum and urine by electrothermal atomic absorption spectrometry using ruthenium as permanent modifier

Analytica Chimica Acta ◽

10.1016/s0003-2670(02)00479-8 ◽

2002 ◽

Vol 464 (2) ◽

pp. 323-330 ◽

Cited By ~ 29

Author(s):

Cristina Gonçalves Magalhães ◽

Kátia Linces Alves Lelis ◽

Clélia Aparecida Rocha ◽

José Bento Borba da Silva

Keyword(s):

Atomic Absorption Spectrometry ◽

Atomic Absorption ◽

Electrothermal Atomic Absorption Spectrometry ◽

Direct Determination ◽

Absorption Spectrometry ◽

Serum And Urine

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Direct determination of chlorpyrifos and its main metabolite 3,5,6-trichloro-2-pyridinol in human serum and urine by coupled-column liquid chromatography/electrospray-tandem mass spectrometry

Rapid Communications in Mass Spectrometry ◽

10.1002/1097-0231(20000830)14:16<1485::aid-rcm51>3.0.co;2-8 ◽

2000 ◽

Vol 14 (16) ◽

pp. 1485-1490 ◽

Cited By ~ 56

Author(s):

Juan V. Sancho ◽

Oscar J. Pozo ◽

F�lix Hern�ndez

Keyword(s):

Mass Spectrometry ◽

Column Liquid Chromatography ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Direct Determination ◽

Tandem Mass ◽

Electrospray Tandem Mass Spectrometry ◽

Main Metabolite ◽

Serum And Urine

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Maltotetraose as a substrate for enzyme-coupled assay of amylase activity in serum and urine.

Clinical Chemistry ◽

10.1093/clinchem/25.3.481 ◽

1979 ◽

Vol 25 (3) ◽

pp. 481-483 ◽

Cited By ~ 16

Author(s):

K J Whitlow ◽

N Gochman ◽

R L Forrester ◽

L J Wataji

Keyword(s):

Amylase Activity ◽

Biological Fluids ◽

Urine Samples ◽

Coupled Assay ◽

Serum And Urine ◽

Serum And Urine Samples

Abstract The use of maltotetraose ss a new substrate for the enzyme-coupled determination of amylase activity in biological fluids was developed by Beckman Microbics. We evaluated a manual and a centrifugal analyzer version of the method in comparison with two commonly used manual starch-dye amylase techniques: Roche Amylochrome and Pharmacia Phadebas. Both maltotetraose amylase procedures proved to be rapid and precise, and results correlated satisfactorily with the starch-dye methods for serum and urine samples.

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Digital quantitative detection of serum circulating miRNAs using dual-enhanced magnetobiosensors based on cascaded nucleic acid circuits

Chemical Communications ◽

10.1039/c9cc07841j ◽

2019 ◽

Vol 55 (91) ◽

pp. 13733-13736 ◽

Cited By ~ 4

Author(s):

Chunxue Liu ◽

Yayun An ◽

Yuanfu Zhang ◽

Xia Li ◽

Qingwang Xue ◽

...

Keyword(s):

Nucleic Acid ◽

Quantitative Detection ◽

Circulating Mirnas ◽

Personal Glucose Meter ◽

Glucose Meter

Here, we developed a dual-enhanced magnetobiosensor based on cascaded nucleic acid circuits for sensitive, portable and digital quantitative detection of circulating miRNAs in serum by a personal glucose meter (PGM).

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