Probing the kinetics of lipid membrane formation and the interaction of a nontoxic and a toxic amyloid with plasmon waveguide resonance

2014 ◽  
Vol 50 (32) ◽  
pp. 4168-4171 ◽  
Author(s):  
E. Harté ◽  
N. Maalouli ◽  
A. Shalabney ◽  
E. Texier ◽  
K. Berthelot ◽  
...  

The kinetics of formation of solid-supported lipid model membranes were investigated using a home-made plasmon waveguide resonance (PWR) sensor possessing enhanced properties relative to classic surface plasmon resonance sensors.

1997 ◽  
Vol 4 (3-4) ◽  
pp. 513-517 ◽  
Author(s):  
Louise M. Williams ◽  
Stephen D. Evans ◽  
Thomas M. Flynn ◽  
Andrew Marsh ◽  
Peter F. Knowles ◽  
...  

2020 ◽  
Vol 108 ◽  
pp. 104728
Author(s):  
Ana Flávia Coelho Pacheco ◽  
Natália Moreira Nunes ◽  
Hauster Maximiler Campos de Paula ◽  
Yara Luiza Coelho ◽  
Luis Henrique Mendes da Silva ◽  
...  

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqin Chen ◽  
Zhiyang Li ◽  
Wenqian Cheng ◽  
Tao Wu ◽  
Jia Li ◽  
...  

AbstractHuman epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated gold nanoparticles (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating a significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of natural enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application. Graphical Abstract


ACS Omega ◽  
2018 ◽  
Vol 3 (7) ◽  
pp. 7133-7140 ◽  
Author(s):  
Nur Selin Kaya ◽  
Anur Yadav ◽  
Michel Wehrhold ◽  
Laura Zuccaro ◽  
Kannan Balasubramanian

2019 ◽  
Vol 175 ◽  
pp. 1-9 ◽  
Author(s):  
Eleni Koutsopoulou ◽  
Aristeidis Papagiannopoulos ◽  
George E. Christidis ◽  
George Tassis ◽  
Nikolaos Spiliopoulos

Biochemistry ◽  
2013 ◽  
Vol 52 (37) ◽  
pp. 6335-6347 ◽  
Author(s):  
Subhashchandra Naik ◽  
Susan Brock ◽  
Narahari Akkaladevi ◽  
Jon Tally ◽  
Wesley Mcginn-Straub ◽  
...  

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