High-throughput colorimetric assays for mercury(ii) in blood and wastewater based on the mercury-stimulated catalytic activity of small silver nanoparticles in a temperature-switchable gelatin matrix

2014 ◽  
Vol 50 (65) ◽  
pp. 9196-9199 ◽  
Author(s):  
Zongzhao Sun ◽  
Ning Zhang ◽  
Yanmei Si ◽  
Shuai Li ◽  
Jiangwei Wen ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Catalytic Activity ◽  
High Throughput ◽  
Gelatin Matrix ◽  
Colorimetric Assays

Colorimetric mercury(ii) assays with 96-cell plates were achieved by the mercury-stimulated catalytic activity of small silver nanoparticles in switchable gelatin.

Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening Towards Improved Catalytic Activity

2019 ◽  
Author(s):  
Huifang Xu ◽  
Weinan Liang ◽  
Linlin Ning ◽  
Yuanyuan Jiang ◽  
Wenxia Yang ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Fatty Acid ◽  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
Colorimetric Detection ◽  
Screening Method ◽  
Random Mutagenesis ◽  
Direct Production ◽  
Consumption Activity

P450 fatty acid decarboxylases (FADCs) have recently been attracting considerable attention owing to their one-step direct production of industrially important 1-alkenes from biologically abundant feedstock free fatty acids under mild conditions. However, attempts to improve the catalytic activity of FADCs have met with little success. Protein engineering has been limited to selected residues and small mutant libraries due to lack of an effective high-throughput screening (HTS) method. Here, we devise a catalase-deficient <i>Escherichia coli</i> host strain and report an HTS approach based on colorimetric detection of H<sub>2</sub>O<sub>2</sub>-consumption activity of FADCs. Directed evolution enabled by this method has led to effective identification for the first time of improved FADC variants for medium-chain 1-alkene production from both DNA shuffling and random mutagenesis libraries. Advantageously, this screening method can be extended to other enzymes that stoichiometrically utilize H<sub>2</sub>O<sub>2</sub> as co-substrate.

Synthesis of N ‐Acetylcysteine Conjugated Cholic Acid Stabilized Gold and Silver Nanoparticles: Evaluation of Their Catalytic Activity and Toxicity Assessment

2021 ◽  
Vol 6 (22) ◽  
pp. 5474-5487
Author(s):  
Nishanthi Ezhumalai ◽  
Manivannan Nanthagopal ◽  
Shanmugam Chandirasekar ◽  
Manikandan Elumalai ◽  
Mathivanan Narayanasamy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Catalytic Activity ◽  
Cholic Acid ◽  
Toxicity Assessment ◽  
Gold And Silver Nanoparticles

scholarly journals Effect of acrylic acid feed contents of microgels on catalytic activity of silver nanoparticles fabricated hybrid microgels

2015 ◽  
Vol 39 ◽  
pp. 96-107 ◽  
Author(s):  
Zahoor H. FAROOQI ◽  
Shanza Rauf KHAN ◽  
Robina BEGUM ◽  
Farah KANWAL ◽  
Ahsan SHARIF ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Catalytic Activity ◽  
Acrylic Acid ◽  
Hybrid Microgels

Fabrication of high-density silver nanoparticles on the surface of alginate microspheres for application in catalytic reaction

2014 ◽  
Vol 2 (22) ◽  
pp. 8491-8499 ◽  
Author(s):  
Jun You ◽  
Chengcheng Zhao ◽  
Jinfeng Cao ◽  
Jinping Zhou ◽  
Lina Zhang
Keyword(s):  
Silver Nanoparticles ◽  
Catalytic Activity ◽  
Catalytic Reaction ◽  
High Density

High-density silver nanoparticles immobilized on the surface of alginate microspheres exhibit excellent catalytic activity by reduction of p-nitrophenol.

scholarly journals Synthesis and Catalytic Activity of Silver Nanoparticles Immobilized onto Composite Microspheres

2013 ◽  
Vol 29 (04) ◽  
pp. 785-791
Author(s):  
HAO Min-Min ◽  
◽  
LI Chen ◽  
YU Min ◽  
ZHANG Ying
Keyword(s):  
Silver Nanoparticles ◽  
Catalytic Activity ◽  
Composite Microspheres

Continuous High-Throughput Colorimetric Assays for α-Transaminases

2017 ◽  
pp. 233-245
Author(s):  
Egon Heuson ◽  
Jean-Louis Petit ◽  
Franck Charmantray ◽  
Véronique de Bérardinis ◽  
Thierry Gefflaut
Keyword(s):  
High Throughput ◽  
Colorimetric Assays

Green synthesis of silver nanoparticles using Piper longum catkin extract irradiated by sunlight: antibacterial and catalytic activity

2019 ◽  
Vol 45 (6) ◽  
pp. 3617-3631 ◽  
Author(s):  
M. Jayapriya ◽  
D. Dhanasekaran ◽  
M. Arulmozhi ◽  
E. Nandhakumar ◽  
N. Senthilkumar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Catalytic Activity ◽  
Green Synthesis ◽  
Piper Longum
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