scholarly journals Capsaicin, nonivamide and trans-pellitorine decrease free fatty acid uptake without TRPV1 activation and increase acetyl-coenzyme A synthetase activity in Caco-2 cells

2015 ◽  
Vol 6 (1) ◽  
pp. 172-184 ◽  
Author(s):  
Barbara Rohm ◽  
Annett Riedel ◽  
Jakob P. Ley ◽  
Sabine Widder ◽  
Gerhard E. Krammer ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Coenzyme A ◽  
Fatty Acid Uptake ◽  
Synthetase Activity ◽  
Acid Uptake ◽  
Structural Element ◽  
Acetyl Coenzyme A ◽  
Acetyl Coenzyme

The effects of capsaicin, nonivamide,trans-pellitorine and vanillin as the basic structural element of all vanilloids on the mechanisms of intestinal fatty acid uptake in differentiated intestinal Caco-2 cells were studied.

scholarly journals Circadian Control of Fatty Acid Elongation by SIRT1 Protein-mediated Deacetylation of Acetyl-coenzyme A Synthetase 1

2014 ◽  
Vol 289 (9) ◽  
pp. 6091-6097 ◽  
Author(s):  
Saurabh Sahar ◽  
Satoru Masubuchi ◽  
Kristin Eckel-Mahan ◽  
Simone Vollmer ◽  
Luisa Galla ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Coenzyme A ◽  
Fatty Acid Elongation ◽  
Acetyl Coenzyme A ◽  
Acetyl Coenzyme ◽  
Circadian Control

Effect of insulin on free fatty acid uptake by hepatocytes in the duck

1984 ◽  
Vol 102 (3) ◽  
pp. 381-386 ◽  
Author(s):  
R. Gross ◽  
P. Mialhe
Keyword(s):  
Fatty Acids ◽  
Growth Hormone ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Glucose Metabolism ◽  
Free Fatty Acids ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Low Doses ◽  
Higher Doses

ABSTRACT To elucidate the hypolipacidaemic effect of insulin in ducks, its action on the uptake of free fatty acids (FFA) by duck hepatocytes was determined. At low doses (10 mu./l) insulin stimulated FFA uptake. This effect was not observed with higher doses of insulin (20, 30 and 50 mu./l). Growth hormone at physiological concentrations and corticosterone (14·4 nmol/l) decreased basal activity, probably by reducing glucose metabolism and consequently α-glycerophosphate (α-GP) supply. Insulin was able to reverse the inhibition induced by GH and corticosterone on both FFA uptake and α-GP production. These results therefore suggest that the hypolipacidaemic effect of insulin may be partly mediated by its action on hepatic FFA uptake. J. Endocr. (1984) 102, 381–386

scholarly journals Biocatalyst Engineering by Assembly of Fatty Acid Transport and Oxidation Activities for In Vivo Application of Cytochrome P-450BM-3 Monooxygenase

1998 ◽  
Vol 64 (10) ◽  
pp. 3784-3790 ◽  
Author(s):  
Silke Schneider ◽  
Marcel G. Wubbolts ◽  
Dominique Sanglard ◽  
Bernard Witholt
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Coenzyme A ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Uptake System ◽  
Whole Cells ◽  
E Coli ◽  
Cytochrome P 450 ◽  
Long Chain

ABSTRACT The application of whole cells containing cytochrome P-450BM-3 monooxygenase [EC 1.14.14.1 ] for the bioconversion of long-chain saturated fatty acids to ω-1, ω-2, and ω-3 hydroxy fatty acids was investigated. We utilized pentadecanoic acid and studied its conversion to a mixture of 12-, 13-, and 14-hydroxypentadecanoic acids by this monooxygenase. For this purpose,Escherichia coli recombinants containing plasmid pCYP102 producing the fatty acid monooxygenase cytochrome P-450BM-3were used. To overcome inefficient uptake of pentadecanoic acid by intact E. coli cells, we made use of a cloned fatty acid uptake system from Pseudomonas oleovorans which, in contrast to the common FadL fatty acid uptake system of E. coli, does not require coupling by FadD (acyl-coenzyme A synthetase) of the imported fatty acid to coenzyme A. This system fromP. oleovorans is encoded by a gene carried by plasmid pGEc47, which has been shown to effect facilitated uptake of oleic acid in E. coli W3110 (M. Nieboer, Ph.D. thesis, University of Groningen, Groningen, The Netherlands, 1996). By using a double recombinant of E. coli K27, which is a fadDmutant and therefore unable to consume substrates or products via the β-oxidation cycle, a twofold increase in productivity was achieved. Applying cytochrome P-450BM-3 monooxygenase as a biocatalyst in whole cells does not require the exogenous addition of the costly cofactor NADPH. In combination with the coenzyme A-independent fatty acid uptake system from P. oleovorans, cytochrome P-450BM-3 recombinants appear to be useful alternatives to the enzymatic approach for the bioconversion of long-chain fatty acids to subterminal hydroxylated fatty acids.

Effect of 2,4-dinitrophenol on Free Fatty Acid Uptake by Skeletal Muscle.

1961 ◽  
Vol 108 (1) ◽  
pp. 89-91 ◽  
Author(s):  
J. J. Spitzer ◽  
W. T. McElroy ◽  
B. Issekutz
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Fatty Acid Uptake ◽  
Acid Uptake

Free fatty-acid uptake by isolated rat hepatocytes

1985 ◽  
Vol 93 (4) ◽  
pp. 313-319 ◽  
Author(s):  
G. Renaud ◽  
M. E. Bouma ◽  
A. Foliot ◽  
R. Infante
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Rat Hepatocytes ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Effect of Norepinephrine on Myocardial Free Fatty Acid Uptake and Oxidation,

1965 ◽  
Vol 118 (4) ◽  
pp. 876-879 ◽  
Author(s):  
M. Gold ◽  
H. J. Attar ◽  
J. J. Spitzer ◽  
J. C. Scott
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Fatty Acid Uptake ◽  
Acid Uptake
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