A droplet-to-digital (D2D) microfluidic device for single cell assays

Lab on a Chip ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 225-236 ◽  
Author(s):  
Steve C. C. Shih ◽  
Philip C. Gach ◽  
Jess Sustarich ◽  
Blake A. Simmons ◽  
Paul D. Adams ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Microfluidic Platform ◽  
Cell Assays ◽  
Digital Microfluidic

We have developed a new hybrid droplet-to-digital microfluidic platform (D2D) that integrates droplet-in-channel microfluidics with digital microfluidics for performing multi-step single cell assays.

Digital microfluidics using a differentially polarized interface (DPI) to enhance translational force

Lab on a Chip ◽  
2018 ◽  
Vol 18 (21) ◽  
pp. 3293-3302 ◽  
Author(s):  
Md Enayet Razu ◽  
Jungkyu Kim
Keyword(s):  
Low Voltage ◽  
Digital Microfluidics ◽  
Microfluidic Platform ◽  
Digital Microfluidic

A low-voltage and differentially polarized digital microfluidic platform is developed by enhancing the electromechanical force for droplet translation.

scholarly journals Microfluidic on-demand droplet generation, storage, retrieval, and merging for single-cell pairing

Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 493-502 ◽  
Author(s):  
Hesam Babahosseini ◽  
Tom Misteli ◽  
Don L. DeVoe
Keyword(s):  
Single Cell ◽  
Aqueous Phase ◽  
Microfluidic Device ◽  
Droplet Generation ◽  
Microfluidic Platform ◽  
On Demand

A multifunctional microfluidic platform combining on-demand aqueous-phase droplet generation, multi-droplet storage, and controlled merging of droplets selected from a storage library in a single integrated microfluidic device is described.

Digital microfluidics for spheroid-based invasion assays

Lab on a Chip ◽  
2016 ◽  
Vol 16 (8) ◽  
pp. 1505-1513 ◽  
Author(s):  
Brian F. Bender ◽  
Andrew. P. Aijian ◽  
Robin. L. Garrell
Keyword(s):  
Drug Discovery ◽  
Cell Invasion ◽  
Cell Biology ◽  
Digital Microfluidics ◽  
Three Dimensional ◽  
Multicellular Spheroids ◽  
Microfluidic Platform ◽  
Digital Microfluidic ◽  
Biology Research ◽  
Invasion Assays

A digital microfluidic platform that enables the formation, gel encapsulation, and assaying of three-dimensional multicellular spheroids is described. Such a platform can facilitate automation of cell invasion assays for cell biology research and drug discovery.

A digital microfluidic device with integrated nanostructured microelectrodes for electrochemical immunoassays

Lab on a Chip ◽  
2015 ◽  
Vol 15 (18) ◽  
pp. 3776-3784 ◽  
Author(s):  
Darius G. Rackus ◽  
Michael D. M. Dryden ◽  
Julian Lamanna ◽  
Alexandre Zaragoza ◽  
Brian Lam ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Digital Microfluidic ◽  
Electrochemical Immunoassays

Nanostructured microelectrodes (NMEs) combined with digital microfluidics (DMF) for automated electroimmunoassays.

scholarly journals Response of single leukemic cells to peptidase inhibitor therapy across time and dose using a microfluidic device

2014 ◽  
Vol 6 (2) ◽  
pp. 164-174 ◽  
Author(s):  
Michelle L. Kovarik ◽  
Alexandra J. Dickinson ◽  
Pourab Roy ◽  
Ranjit A. Poonnen ◽  
Jason P. Fine ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Biological Effects ◽  
Regression Modeling ◽  
Inhibitor Therapy ◽  
Leukemic Cells ◽  
Peptide Degradation ◽  
Cell Assays ◽  
Peptidase Inhibitor

Microfluidic single-cell assays of peptide degradation were performed at varying inhibitor doses, and the resulting data were analyzed by regression modeling to reveal biological effects.

Method for Characterization of Passive Mechanical Filtration of Particles in Digital Microfluidic Devices

2014 ◽  
Author(s):  
Peter D. Dunning ◽  
Pierre E. Sullivan ◽  
Michael J. Schertzer
Keyword(s):  
Microfluidic Device ◽  
Vision System ◽  
Low Cost ◽  
Digital Microfluidics ◽  
Microfluidic Devices ◽  
Comb Filter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reaction Site ◽  
Digital Microfluidic

The ability to remove unbound biological material from a reaction site has applications in many biological protocols, such as those used to detect pathogens and biomarkers. One specific application where washing is critical is the Enzyme-Linked ImmunoSorbent Assay (ELISA). This protocol requires multiple washing steps to remove multiple reagents from a reaction site. Previous work has suggested that a passive mechanical comb filter can be used to wash particles in digital microfluidic devices. A method for the characterization of passive mechanical filtration of particles in Digital MicroFluidic (DMF) devices is presented in this work. In recent years there has been increased development of Lab-On-A-Chip (LOAC) devices for the automation and miniaturization of biological protocols. One platform for further research is in digital microfluidics. A digital microfluidic device can control the movement of pico-to nanoliter droplets of fluid using electrical signals without the use of pumps, valves, and channels. As such, fluidic pathways are not hardwired and the path of each droplet can be easily reconfigured. This is advantageous in biological protocols requiring the use of multiple reagents. Fabrication of these devices is relatively straight forward, since fluid manipulation is possible without the use of complex components. This work presents a method to characterize the performance of a digital microfluidic device using passive mechanical supernatant dilution via image analysis using a low cost vision system. The primary metric for performance of the device is particle retention after multiple passes through the filter. Repeatability of the process will be examined by characterizing performance of multiple devices using the same filter geometry. Qualitative data on repeatability and effectiveness of the dilution technique will also be attained by observing the ease with which the droplet disengages from the filter and by measuring the quantity of fluid trapped on the filter after each filtration step.

Analytical Models to Determine the Electric Field Characteristics of a Multi-Electrode Impedimetric Immunosensor in a Digital Microfluidic Device

2014 ◽  
Author(s):  
Steffen O. P. Blume ◽  
Michael J. Schertzer ◽  
Ridha Ben Mrad ◽  
Pierre E. Sullivan
Keyword(s):  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Electrode Array ◽  
Analytical Models ◽  
Element Analysis ◽  
Numerical Simulation Methods ◽  
Mapping Techniques ◽  
On Chip ◽  
Digital Microfluidic ◽  
Impedimetric Immunosensor

The level of integration of digital microfluidics is continually increasing to include the system path from fluid manipulation and transport, on to reagent preparation, and finally reaction detection. Digital microfluidics therefore has the capability to encompass all steps of common biochemical protocols. Reported here is a set of analytical models for the design of a coplanar interdigitated multi-electrode array to be used as an impedimetric immunosensor in a digital microfluidic device for on-chip chemical reaction detection. The models are based on conformal mapping techniques, and are compared to results obtained from finite element analysis to discuss limitations of the model. The analytical models are feasible and inexpensive surrogates for numerical simulation methods.

scholarly journals Digital Microfluidic Platform for Multiplexing Enzyme Assays: Implications for Lysosomal Storage Disease Screening in Newborns

2011 ◽  
Vol 57 (10) ◽  
pp. 1444-1451 ◽  
Author(s):  
Ramakrishna S Sista ◽  
Allen E Eckhardt ◽  
Tong Wang ◽  
Carrie Graham ◽  
Jeremy L Rouse ◽  
...  
Keyword(s):  
Newborn Screening ◽  
Incubation Time ◽  
Digital Microfluidics ◽  
Dried Blood Spots ◽  
Reference Laboratory ◽  
Enzymatic Analysis ◽  
Lysosomal Storage ◽  
Microfluidic Platform ◽  
Enzyme Replacement ◽  
Digital Microfluidic

BACKGROUND Newborn screening for lysosomal storage diseases (LSDs) has been gaining considerable interest owing to the availability of enzyme replacement therapies. We present a digital microfluidic platform to perform rapid, multiplexed enzymatic analysis of acid α-glucosidase (GAA) and acid α-galactosidase to screen for Pompe and Fabry disorders. The results were compared with those obtained using standard fluorometric methods. METHODS We performed bench-based, fluorometric enzymatic analysis on 60 deidentified newborn dried blood spots (DBSs), plus 10 Pompe-affected and 11 Fabry-affected samples, at Duke Biochemical Genetics Laboratory using a 3-mm punch for each assay and an incubation time of 20 h. We used a digital microfluidic platform to automate fluorometric enzymatic assays at Advanced Liquid Logic Inc. using extract from a single punch for both assays, with an incubation time of 6 h. Assays were also performed with an incubation time of 1 h. RESULTS Assay results were generally comparable, although mean enzymatic activity for GAA using microfluidics was approximately 3 times higher than that obtained using bench-based methods, which could be attributed to higher substrate concentration. Clear separation was observed between the normal and affected samples at both 6- and 1-h incubation times using digital microfluidics. CONCLUSIONS A digital microfluidic platform compared favorably with a clinical reference laboratory to perform enzymatic analysis in DBSs for Pompe and Fabry disorders. This platform presents a new technology for a newborn screening laboratory to screen LSDs by fully automating all the liquid-handling operations in an inexpensive system, providing rapid results.

scholarly journals Magnetic digital microfluidics – a review

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 994-1008 ◽  
Author(s):  
Yi Zhang ◽  
Nam-Trung Nguyen
Keyword(s):  
Digital Microfluidics ◽  
Open Surface ◽  
Microfluidic Platform ◽  
Digital Microfluidic

A magnetic digital microfluidic platform manipulates droplets on an open surface.

An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting

Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 524-535 ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Kenza Samlali ◽  
Philippe Q. N. Vo ◽  
Steve C. C. Shih
Keyword(s):  
Ionic Liquid ◽  
Digital Microfluidics ◽  
Microfluidic System ◽  
Yeast Mutant ◽  
Microfluidic Platform ◽  
On Demand ◽  
Digital Microfluidic ◽  
Mutant Cells

A new microfluidic platform that integrates droplet and digital microfluidics to automate a variety of fluidic operations. The platform was applied to culturing and to selecting yeast mutant cells in ionic liquid.

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