Digital microfluidics for spheroid-based invasion assays

Lab on a Chip ◽  
2016 ◽  
Vol 16 (8) ◽  
pp. 1505-1513 ◽  
Author(s):  
Brian F. Bender ◽  
Andrew. P. Aijian ◽  
Robin. L. Garrell
Keyword(s):  
Drug Discovery ◽  
Cell Invasion ◽  
Cell Biology ◽  
Digital Microfluidics ◽  
Three Dimensional ◽  
Multicellular Spheroids ◽  
Microfluidic Platform ◽  
Digital Microfluidic ◽  
Biology Research ◽  
Invasion Assays

A digital microfluidic platform that enables the formation, gel encapsulation, and assaying of three-dimensional multicellular spheroids is described. Such a platform can facilitate automation of cell invasion assays for cell biology research and drug discovery.

Analysis of the effects of aryl hydrocarbon receptor expression on cancer cell invasion via three-dimensional microfluidic invasion assays

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Bingyu B. Li ◽  
Erica Y. Scott ◽  
Ninni E. Olafsen ◽  
Jason Matthews ◽  
Aaron R. Wheeler
Keyword(s):  
Cell Invasion ◽  
Three Dimensional ◽  
Transcriptome Profiling ◽  
Receptor Expression ◽  
Unique Combination ◽  
Cancer Cell Invasion ◽  
Aryl Hydrocarbon ◽  
Functional Discrimination ◽  
Digital Microfluidic ◽  
Invasion Assays

We studied the effect of AHR expression on metastasis using cell invasion in digital microfluidic microgel systems (CIMMS), which provided a unique combination of functional discrimination with transcriptome profiling of sub-populations of cells.

Digital microfluidics using a differentially polarized interface (DPI) to enhance translational force

Lab on a Chip ◽  
2018 ◽  
Vol 18 (21) ◽  
pp. 3293-3302 ◽  
Author(s):  
Md Enayet Razu ◽  
Jungkyu Kim
Keyword(s):  
Low Voltage ◽  
Digital Microfluidics ◽  
Microfluidic Platform ◽  
Digital Microfluidic

A low-voltage and differentially polarized digital microfluidic platform is developed by enhancing the electromechanical force for droplet translation.

A droplet-to-digital (D2D) microfluidic device for single cell assays

Lab on a Chip ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 225-236 ◽  
Author(s):  
Steve C. C. Shih ◽  
Philip C. Gach ◽  
Jess Sustarich ◽  
Blake A. Simmons ◽  
Paul D. Adams ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Microfluidic Platform ◽  
Cell Assays ◽  
Digital Microfluidic

We have developed a new hybrid droplet-to-digital microfluidic platform (D2D) that integrates droplet-in-channel microfluidics with digital microfluidics for performing multi-step single cell assays.

scholarly journals Digital Microfluidic Platform for Multiplexing Enzyme Assays: Implications for Lysosomal Storage Disease Screening in Newborns

2011 ◽  
Vol 57 (10) ◽  
pp. 1444-1451 ◽  
Author(s):  
Ramakrishna S Sista ◽  
Allen E Eckhardt ◽  
Tong Wang ◽  
Carrie Graham ◽  
Jeremy L Rouse ◽  
...  
Keyword(s):  
Newborn Screening ◽  
Incubation Time ◽  
Digital Microfluidics ◽  
Dried Blood Spots ◽  
Reference Laboratory ◽  
Enzymatic Analysis ◽  
Lysosomal Storage ◽  
Microfluidic Platform ◽  
Enzyme Replacement ◽  
Digital Microfluidic

BACKGROUND Newborn screening for lysosomal storage diseases (LSDs) has been gaining considerable interest owing to the availability of enzyme replacement therapies. We present a digital microfluidic platform to perform rapid, multiplexed enzymatic analysis of acid α-glucosidase (GAA) and acid α-galactosidase to screen for Pompe and Fabry disorders. The results were compared with those obtained using standard fluorometric methods. METHODS We performed bench-based, fluorometric enzymatic analysis on 60 deidentified newborn dried blood spots (DBSs), plus 10 Pompe-affected and 11 Fabry-affected samples, at Duke Biochemical Genetics Laboratory using a 3-mm punch for each assay and an incubation time of 20 h. We used a digital microfluidic platform to automate fluorometric enzymatic assays at Advanced Liquid Logic Inc. using extract from a single punch for both assays, with an incubation time of 6 h. Assays were also performed with an incubation time of 1 h. RESULTS Assay results were generally comparable, although mean enzymatic activity for GAA using microfluidics was approximately 3 times higher than that obtained using bench-based methods, which could be attributed to higher substrate concentration. Clear separation was observed between the normal and affected samples at both 6- and 1-h incubation times using digital microfluidics. CONCLUSIONS A digital microfluidic platform compared favorably with a clinical reference laboratory to perform enzymatic analysis in DBSs for Pompe and Fabry disorders. This platform presents a new technology for a newborn screening laboratory to screen LSDs by fully automating all the liquid-handling operations in an inexpensive system, providing rapid results.

scholarly journals Magnetic digital microfluidics – a review

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 994-1008 ◽  
Author(s):  
Yi Zhang ◽  
Nam-Trung Nguyen
Keyword(s):  
Digital Microfluidics ◽  
Open Surface ◽  
Microfluidic Platform ◽  
Digital Microfluidic

A magnetic digital microfluidic platform manipulates droplets on an open surface.

An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting

Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 524-535 ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Kenza Samlali ◽  
Philippe Q. N. Vo ◽  
Steve C. C. Shih
Keyword(s):  
Ionic Liquid ◽  
Digital Microfluidics ◽  
Microfluidic System ◽  
Yeast Mutant ◽  
Microfluidic Platform ◽  
On Demand ◽  
Digital Microfluidic ◽  
Mutant Cells

A new microfluidic platform that integrates droplet and digital microfluidics to automate a variety of fluidic operations. The platform was applied to culturing and to selecting yeast mutant cells in ionic liquid.

Exploration of cell wall architecture with the rapid-freeze deep-etch technique

1993 ◽  
Vol 51 ◽  
pp. 128-129
Author(s):  
Béatrice Satiat-Jeunemaitre ◽  
Chris Hawes
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Cell Biology ◽  
Molecular Model ◽  
Three Dimensional ◽  
Secretory Activity ◽  
Wall Structure ◽  
Specimen Preparation ◽  
Molecular Architecture ◽  
Plant Cell Walls

The comprehension of the molecular architecture of plant cell walls is one of the best examples in cell biology which illustrates how developments in microscopy have extended the frontiers of a topic. Indeed from the first electron microscope observation of cell walls it has become apparent that our understanding of wall structure has advanced hand in hand with improvements in the technology of specimen preparation for electron microscopy. Cell walls are sub-cellular compartments outside the peripheral plasma membrane, the construction of which depends on a complex cellular biosynthetic and secretory activity (1). They are composed of interwoven polymers, synthesised independently, which together perform a number of varied functions. Biochemical studies have provided us with much data on the varied molecular composition of plant cell walls. However, the detailed intermolecular relationships and the three dimensional arrangement of the polymers in situ remains a mystery. The difficulty in establishing a general molecular model for plant cell walls is also complicated by the vast diversity in wall composition among plant species.

Three-dimensional Growth of Renal Epithelial Cells in Vitro: A Tool in Toxicity Testing

1993 ◽  
Vol 21 (2) ◽  
pp. 191-195 ◽  
Author(s):  
Knut-Jan Andersen ◽  
Erik Ilsø Christensen ◽  
Hogne Vik
Keyword(s):  
Epithelial Cells ◽  
Three Dimensional ◽  
Toxicity Testing ◽  
Renal Tissue ◽  
Epithelial Cell Line ◽  
Multicellular Spheroids ◽  
Renal Epithelial Cell ◽  
Renal Epithelial Cells

The tissue culture of multicellular spheroids from the renal epithelial cell line LLC-PK1 (proximal tubule) is described. This represents a biological system of intermediate complexity between renal tissue in vivo and simple monolayer cultures. The multicellular structures, which show many similarities to kidney tubules in vivo, including a vectorial water transport, should prove useful for studying the potential nephrotoxicity of drugs and chemicals in vitro. In addition, the propagation of renal epithelial cells as multicellular spheroids in serum-free culture may provide information on the release of specific biological parameters, which may be suppressed or masked in serum-supplemented media.

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