Development of reactive extraction systems for itaconic acid: a step towards in situ product recovery for itaconic acid fermentation

RSC Advances ◽  
2014 ◽  
Vol 4 (85) ◽  
pp. 45029-45039 ◽  
Author(s):  
Guneet Kaur ◽  
Kathy Elst
Keyword(s):  
Process Optimization ◽  
Itaconic Acid ◽  
Acid Production ◽  
Product Recovery ◽  
Reactive Extraction ◽  
Acid Fermentation ◽  
In Situ Product Recovery ◽  
Product Separation ◽  
Important Means

Process optimization by integration of bioconversion with product separation and recovery i.e. in situ product recovery (ISPR) is an important means to develop a sustainable and petrochemical-competitive biotechnological method for itaconic acid production.

scholarly journals In Situ Product Recovery of Bio-Based Industrial Platform Chemicals: A Guideline to Solvent Selection

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 26
Author(s):  
Pieter De Brabander ◽  
Evelien Uitterhaegen ◽  
Ellen Verhoeven ◽  
Cedric Vander Cruyssen ◽  
Karel De Winter ◽  
...  
Keyword(s):  
Organic Acids ◽  
Distribution Coefficients ◽  
Product Recovery ◽  
Reactive Extraction ◽  
Solvent Mixtures ◽  
In Situ Product Recovery ◽  
Platform Chemicals ◽  
Highly Hydrophobic ◽  
Fragrance Compounds

In situ product recovery (ISPR), in the form of an extractive fermentation process, can increase productivity and product titers in the sustainable production of platform chemicals. To establish a guideline for the development of industrially relevant production processes for such bio-based compounds, a wide screening was performed, mapping the potential of an extensive range of solvents and solvent mixtures. Besides solvent biocompatibility with Saccharomyces cerevisiae, distribution coefficients of three organic acids (protocatechuic acid, adipic acid and para-aminobenzoic acid) and four fragrance compounds (2-phenylethanol, geraniol, trans-cinnamaldehyde and β-ionone) were determined. While for highly hydrophobic fragrance compounds, multiple pure solvents were identified that were able to extract more than 98%, reactive extraction mixtures were proven effective for more challenging compounds including organic acids and hydrophilic alcohols. For example, a reactive mixture consisting of 12.5% of the extractant CYTOP 503 in canola oil was found to be biocompatible and showed superior extraction efficiency for the challenging compounds as compared to any biocompatible single solvent. This mapping of biocompatible solvents and solvent mixtures for the extraction of various classes of industrial platform chemicals can be a tremendous step forward in the development of extractive fermentations.

Enhanced malic acid production using Aspergillus niger coupled with in situ product recovery

2020 ◽  
Vol 308 ◽  
pp. 123259
Author(s):  
J. Iyyappan ◽  
G. Baskar ◽  
B. Bharathiraja ◽  
M. Gopinath
Keyword(s):  
Aspergillus Niger ◽  
Malic Acid ◽  
Acid Production ◽  
Product Recovery ◽  
In Situ Product Recovery

scholarly journals A comparison of the energy use of in situ product recovery techniques for the Acetone Butanol Ethanol fermentation

2016 ◽  
Vol 220 ◽  
pp. 590-600 ◽  
Author(s):  
Victoria Outram ◽  
Carl-Axel Lalander ◽  
Jonathan G.M. Lee ◽  
E. Timothy Davis ◽  
Adam P. Harvey
Keyword(s):  
Ethanol Fermentation ◽  
Energy Use ◽  
Product Recovery ◽  
In Situ Product Recovery ◽  
Recovery Techniques ◽  
Acetone Butanol Ethanol

scholarly journals Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3356 ◽  
Author(s):  
Francisco Aguilar ◽  
Thomas Scheper ◽  
Sascha Beutel
Keyword(s):  
Liquid Phase ◽  
Product Recovery ◽  
Expanded Bed Adsorption ◽  
Biotechnological Production ◽  
Promising Alternative ◽  
Phase Partitioning ◽  
In Situ Product Recovery ◽  
Solid Liquid ◽  
In Situ Recovery

The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in nearly 20% of men’s fine perfumery. The biotechnological production of such fragrant sesquiterpenes is a promising alternative towards sustainability; nevertheless, product recovery from fermentation is one of the main constraints. In an effort to improve the (+)-zizaene recovery from a metabolically-engineered Escherichia coli, we developed an integrated bioprocess by coupling fermentation and (+)-zizaene recovery using adsorber extractants. Initially, (+)-zizaene volatilization was confirmed from cultivations with no extractants but application of liquid–liquid phase partitioning cultivation (LLPPC) improved (+)-zizaene recovery nearly 4-fold. Furthermore, solid–liquid phase partitioning cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion HP20 reached the highest recovery. Bioprocess was scaled up to 2 L bioreactors and in situ recovery configurations integrated to fermentation were evaluated. External recovery configuration was performed with an expanded bed adsorption column and improved (+)-zizaene titers 2.5-fold higher than LLPPC. Moreover, internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 2.2-fold, whereas adsorption velocity was determined as critical parameter for recovery efficiency. Consequently, IRC improved the (+)-zizaene titer 8.4-fold and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 211.13 mg L−1 and productivity of 3.2 mg L−1 h−1. This study provides further knowledge for integration of terpene bioprocesses by in situ product recovery, which could be applied for many terpene studies towards the industrialization of fragrant molecules.

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