Characterization of carbon-coated magnetic nanoparticles using clinical blood coagulation assays: effect of PEG-functionalization and comparison to silica nanoparticles

In this study, a microscale well-plate colorimetric assay for the multiplexed detection of glucose and cholesterol in clinical human blood samples has been developed. This system utilized onepot nanocomposite entrapping Fe3O4 magnetic nanoparticles (MNPs) as peroxidase mimetics and glucose oxidase (GOx)/cholesterol oxidase (ChOx) in mesoporous silica to detect glucose and cholesterol in blood samples. The sensing mechanism involves the generation of H2O2 by the catalytic action of an immobilized oxidase on the target molecules in the sample. This subsequently activates the MNPs in the mesopores, thereby leading to the conversion of the substrate into a colored end product. This strategy is used to detect the target glucose or cholesterol molecules in the concentration range of 15–250 mg/dL. The response is highly linear and the lower detection limit is 7.5 mg/dL. The aforementioned colorimetric assay is extremely convenient, and it exhibits a high degree of linearity, precision, and reproducibility when employing real human blood samples. Therefore, this assay can be used in clinical practice for the multiplexed and reliable quantification of glucose and cholesterol.

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Frequency characterization of dielectric parameters and impedance of irradiated diabetic blood using He-Ne laser and non-diabetic human blood samples

Journal of Physics Conference Series ◽

10.1088/1742-6596/1892/1/012024 ◽

2021 ◽

Vol 1892 (1) ◽

pp. 012024

Author(s):

Sylvester J. Gemanam ◽

Nursakinah Suardi ◽

Barnabas A. Ikyo ◽

Samson Damilola Oluwafemi

Keyword(s):

Human Blood ◽

Dielectric Parameters ◽

Blood Samples ◽

Human Blood Samples

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Characterization of Five Zoonotic Streptococcus suis Strains from Germany, Including One Isolate from a Recent Fatal Case of Streptococcal Toxic Shock-Like Syndrome in a Hunter

Journal of Clinical Microbiology ◽

10.1128/jcm.02578-15 ◽

2015 ◽

Vol 53 (12) ◽

pp. 3912-3915 ◽

Cited By ~ 6

Author(s):

Tobias Eisenberg ◽

Christoph Hudemann ◽

Hamid M. Hossain ◽

Angela Hewer ◽

Khodr Tello ◽

...

Keyword(s):

Human Blood ◽

Ex Vivo ◽

Fatal Case ◽

Streptococcus Suis ◽

Toxic Shock ◽

Blood Samples ◽

Content Type ◽

Human Blood Samples ◽

Low Levels

AStreptococcus suisisolate from a German hunter with streptococcal toxic shock-like syndrome (STSLS) and four additional zoonotic isolates were genotyped asmrp+epf* (variant 1890)sly+cps2+. All five zoonotic German strains were characterized by high multiplication in human blood samplesex vivo, but induction of only low levels of proinflammatory cytokines compared to a Chinese STSLS strain.

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Anticoagulant Activity of Dayak Onion Bulb (Eleutherine bulbosa) Extract on Human Blood Samples

Health Notions ◽

10.33846/hn50202 ◽

2021 ◽

Vol 5 (2) ◽

pp. 46-48

Author(s):

Anik Handayati ◽

Syamsul Arifin ◽

Monica Putji Astuti

Keyword(s):

Blood Coagulation ◽

Human Blood ◽

Anticoagulant Activity ◽

Onion Bulb ◽

Clotting Time ◽

Coagulation Time ◽

Blood Samples ◽

Vein Thrombosis ◽

Onion Bulbs ◽

Human Blood Samples

Cardiovascular disease can occur due to disruption of the hemostatic system by forming blood clots in blood vessels, or commonly known as deep vein thrombosis. The curative action that has been carried out in the form of heparin anticoagulant administration had many side effects such as bleeding, hematuria, thrombocytopenia, and hypersensitivity; therefore, alternative anticoagulant ingredients such as onion bulbs have emerged. Dayak onion bulb (Eleutherine bulbosa) is known to contain eleutherinol compound, which has anticoagulant activity. This study aims to determine the potential anticoagulant activity of Dayak onion bulbs extract (Eleutherine bulbosa) on human blood samples. This anticoagulant activity test was carried out by observing the length of time for blood clotting in the extract treatment group with several concentrations (0, 10, 12.5, 16, 25, and 50%) by Clotting Time (Lee-White) method. The results showed a significant delay in blood coagulation time in the group treated with Dayak onion bulbs extract as increasing the concentration. About 50% Dayak onion onion bulb extract was showed a twofold increase in blood coagulation time thanthat of no extract administration. So that, Dayak bulb onion has the opportunity to be an alternative natural anticoagulant. Keywords: anticoagulants; Dayak onion bulbs (Eleutherine bulbosa); clotting time

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