Formation of copper nanoparticles on poly(thymine) through surface-initiated enzymatic polymerization and its application for DNA detection

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5678-5684 ◽  
Author(s):  
Weiwen Hu ◽  
Yong Ning ◽  
Jinming Kong ◽  
Xueji Zhang
Keyword(s):  
Fluorescent Probe ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Dna Detection ◽  
Dna Biosensor ◽  
Enzymatic Polymerization ◽  
Amplification Strategy

A DNA biosensor using polyT-templated CuNPs as the fluorescent probe and SIEP as the signal amplification strategy is proposed.

Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6689-6697 ◽  
Author(s):  
Han Zhao ◽  
Ying Yan ◽  
Mingjian Chen ◽  
Tingting Hu ◽  
Kefeng Wu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Method ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A facile fluorescent method has been developed for polynucleotide kinase detection based on copper nanoparticles and exonuclease III-assisted signal amplification.

scholarly journals Sensitive DNA detection using cascade amplification strategy based on conjugated polyelectrolytes and hybridization chain reaction

RSC Advances ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3528-3533 ◽  
Author(s):  
Biqing Bao ◽  
Jin Zhu ◽  
Lina Gong ◽  
Jia Chen ◽  
Yanrui Pan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Detection ◽  
Hybridization Chain Reaction ◽  
Molecular Wire ◽  
Conjugated Polyelectrolytes ◽  
Chain Reaction ◽  
Amplification Strategy ◽  
Cascade Amplification

A novel cascade amplification strategy that combines the molecular wire effects of CPEs with the signal amplification capability of the HCR has been developed for sensitive DNA detection.

An electrochemical aptasensor for detection of IFN-γ using graphene and a dual signal amplification strategy based on the exonuclease-mediated surface-initiated enzymatic polymerization

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7784-7791 ◽  
Author(s):  
Chang Liu ◽  
Guiming Xiang ◽  
Dongneng Jiang ◽  
Linlin Liu ◽  
Fei Liu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Enzymatic Polymerization ◽  
Electrochemical Aptasensor ◽  
Target Recycling ◽  
Ifn Γ ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A novel aptasensor based on exonuclease-catalyzed target recycling and surface-initiated enzymatic polymerization for amplification.

A new photoelectrochemical biosensor for ultrasensitive determination of nucleic acids based on a three-stage cascade signal amplification strategy

The Analyst ◽  
2018 ◽  
Vol 143 (12) ◽  
pp. 2799-2806 ◽  
Author(s):  
Erhu Xiong ◽  
Xiaoxia Yan ◽  
Xiaohua Zhang ◽  
Yanmei Li ◽  
Ruiying Yang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Dna Detection ◽  
Amplification Method ◽  
Photoelectrochemical Biosensor ◽  
Amplification Strategy

Based on a “signal-on” sensing strategy and a three-stage cascade signal amplification method, an ultrasensitive photoelectrochemical biosensor has been developed for DNA detection.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Sign in / Sign up

Export Citation Format

Share Document