scholarly journals Temperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads

The Analyst ◽  
2016 ◽  
Vol 141 (3) ◽  
pp. 910-917 ◽  
Author(s):  
Yuki Hiruta ◽  
Ryosuke Kanazashi ◽  
Eri Ayano ◽  
Teruo Okano ◽  
Hideko Kanazawa
Keyword(s):  
Aqueous Solution ◽  
Molecular Recognition ◽  
Temperature Response ◽  
Selective Separation ◽  
Separation System ◽  
Modified Silica ◽  
Temperature Responsive ◽  
Silica Beads

The separation system with dual temperature-response and molecular recognition characteristics leads to selective separation using only aqueous solution.

Temperature-modulated cell-separation column using temperature-responsive cationic copolymer hydrogel-modified silica beads

2019 ◽  
Vol 178 ◽  
pp. 253-262 ◽  
Author(s):  
Kenichi Nagase ◽  
Daimu Inanaga ◽  
Daiju Ichikawa ◽  
Aya Mizutani Akimoto ◽  
Yutaka Hattori ◽  
...  
Keyword(s):  
Cell Separation ◽  
Modified Silica ◽  
Copolymer Hydrogel ◽  
Temperature Responsive ◽  
Separation Column ◽  
Silica Beads

scholarly journals Temperature responsive chromatography for therapeutic drug monitoring with an aqueous mobile phase

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kenichi Nagase ◽  
Teruno Nishiyama ◽  
Masakazu Inoue ◽  
Hideko Kanazawa
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Organic Solvents ◽  
Drug Monitoring ◽  
Mobile Phase ◽  
Therapeutic Drug ◽  
Modified Silica ◽  
Chromatography Column ◽  
Temperature Responsive ◽  
Silica Beads ◽  
Elution Behavior

AbstractTherapeutic drug monitoring is a key technology for effective pharmacological treatment. In the present study, a temperature-responsive chromatography column was developed for safe and simple therapeutic drug monitoring without the use of organic solvents. Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel-modified silica beads were prepared via a condensation reaction and radical polymerization. The temperature-dependent elution behavior of the drugs was observed using a PNIPAAm-modified silica-bead packed column and an all-aqueous mobile phase. Sharp peaks with reproducible retention times were observed at temperatures of 30 °C or 40 °C because the PNIPAAm hydrogel on the silica beads shrinks at these temperatures, limiting drug diffusion into the PNIPAAm hydrogel layer. The elution behavior of the sample from the prepared column was examined using a mixture of serum and model drugs. The serum and drugs were separated on the column at 30 °C or 40 °C, and the concentration of the eluted drug was obtained using the calibration curve. The results show that the prepared chromatography column would be useful for therapeutic drug monitoring because the drug concentration in serum can be measured without using organic solvents in the mobile phase and without any need for sample preparation.

Protein purification using solid-phase extraction on temperature-responsive hydrogel-modified silica beads

2018 ◽  
Vol 1568 ◽  
pp. 38-48 ◽  
Author(s):  
Kohei Okubo ◽  
Koji Ikeda ◽  
Ayaka Oaku ◽  
Yuki Hiruta ◽  
Kenichi Nagase ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Protein Purification ◽  
Solid Phase ◽  
Phase Extraction ◽  
Modified Silica ◽  
Temperature Responsive ◽  
Silica Beads

A novel immobilized trypsin on hydrophilic polymer modified silica beads for proteome characterization

2013 ◽  
Vol 31 (5) ◽  
pp. 423
Author(s):  
Chao FAN ◽  
Zifeng SONG ◽  
Weijie QIN ◽  
Yun CAI ◽  
Xiaohong QIAN
Keyword(s):  
Hydrophilic Polymer ◽  
Modified Silica ◽  
Immobilized Trypsin ◽  
Silica Beads

Molecular Recognition by Steroid Cyclophane in Aqueous Solution and Synthetic Bilayer Membrane

1991 ◽  
Vol 20 (10) ◽  
pp. 1807-1810 ◽  
Author(s):  
Jun-ichi Kikuchi ◽  
Chiemi Matsushima ◽  
Kazuaki Suehiro ◽  
Rie Oda ◽  
Yukito Murakami
Keyword(s):  
Aqueous Solution ◽  
Molecular Recognition ◽  
Bilayer Membrane
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