Lab on a chip sensor for rapid detection and antibiotic resistance determination of Staphylococcus aureus

The Analyst ◽  
2016 ◽  
Vol 141 (6) ◽  
pp. 1922-1929 ◽  
Author(s):  
Chathurika D. Abeyrathne ◽  
Duc H. Huynh ◽  
Thomas W. Mcintire ◽  
Thanh C. Nguyen ◽  
Babak Nasr ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Silicon Dioxide ◽  
Rapid Detection ◽  
Lab On A Chip ◽  
Interdigitated Electrode ◽  
Electrode Sensor

Silicon dioxide passivated, interdigitated electrode sensor for rapid detection and antibiotic resistance determination of S. aureus.

STAPHYLOCOCCUS AUREUS SENSITIVITY TO ANTIBIOTICS IN CHILDREN WITH ATOPIC DERMATITIS

2017 ◽  
Vol 14 (6) ◽  
pp. 21-25
Author(s):  
E E Varlamov ◽  
E S Fedenko ◽  
M C Treneva ◽  
V R Voronina ◽  
Z V Zaporozhtseva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atopic Dermatitis ◽  
Antibiotic Resistance ◽  
Secondary Infection ◽  
Skin Scraping ◽  
Microbiological Examination ◽  
Antibiotics Sensitivity

Background. The aim of this study was to assess the antibiotic resistance of S. aureus isolated from the skin of atopic dermatitis (AD) children. Materials and methods. 256 AD children were included in the study in 2014-2016. Microbiological examination of skin scraping and determination of antibiotics sensitivity were performed. Results. Staphylococcus aureus was isolated in 173 (67,6%) of 256 patients. Resistance to erythromycin was established in 64,6 and 74,6%, respectively. Retrospective analyses showed that S. aureus became more sensitive to ampicillin, ceftriaxone, ciprofloxacin but more resistant to erythromycin. Conclusion. Ceftriaxone and cefoxitin but not erythromycin and ampicillin are the drugs of choice for the treatment of atopic dermatitis complicated by secondary infection in children.

Enterotoxigenic and Antibiotic Resistance Determination of Staphylococcus aureus Strains Isolated from Food Handlers in Gaborone, Botswana

2007 ◽  
Vol 70 (12) ◽  
pp. 2764-2768 ◽  
Author(s):  
DANIEL LOETO ◽  
M. I. MATSHEKA ◽  
B. A. GASHE
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Nasal Cavity ◽  
Penicillin G ◽  
Food Handlers ◽  
Carriage Rate ◽  
Methicillin Resistant ◽  
Resistant Strains ◽  
High Standards

The prevalence, antibiotic resistance, and enterotoxigenic potential of Staphylococcus aureus strains from different anatomical sites on food handlers in Gaborone, Botswana, were determined. Of a total of 200 food handlers tested, 115 (57.5%) were positive for S. aureus. Of the 204 S. aureus isolates, 63 (30.9%), 91 (44.6%), and 50 (24.5%) were isolated from the hand, nasal cavity, and face, respectively, and 43 (21%) of the isolates were enterotoxigenic. The most prevalent enterotoxin was type A, which accounted for 34.9% of all the enterotoxigenic strains, and enterotoxin D was produced by the fewest number of strains (9.3%). Resistance to methicillin was encountered in 33 (22.4%) of the penicillin G–resistant isolates, and 9 (27.3%) of these methicillin-resistant isolates also were resistant to vancomycin. Nineteen antibiotic resistance profiles were determined, and the nasal cavity had the highest diversity of resistance profiles. The nasal cavity also had the highest number of resistant strains, 77 (53%), whereas the hand and face had 49 (32%) and 24 (16.0%) resistant strains, respectively. To reduce the Staphylococcus carriage rate among food handlers, training coupled with a commitment to high standards of personal and environmental hygiene is recommended.

Cloning and Nucleotide Sequence Determination of the Entire mec DNA of Pre-Methicillin-Resistant Staphylococcus aureus N315

1999 ◽  
Vol 43 (6) ◽  
pp. 1449-1458 ◽  
Author(s):  
T. Ito ◽  
Y. Katayama ◽  
K. Hiramatsu
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Methicillin Resistance ◽  
Integration Site ◽  
Bacterial Species ◽  
Antibiotic Resistance Genes ◽  
Direct Repeat ◽  
Methicillin Resistant ◽  
Reading Frame

ABSTRACT In methicillin-resistant Staphylococcus aureus, the methicillin resistance gene mecA is localized within a large chromosomal region which is absent in the methicillin-susceptibleS. aureus chromosome. The region, designatedmec DNA, is speculated to have originated from the genome of another bacterial species and become integrated into the chromosome of the S. aureus cell in the past. We report here cloning and determination of the structure of the entire mec DNA sequence from a Japanese S. aureus strain, N315. Themec DNA was found to be 51,669 bp long, including terminal inverted repeats of 27 bp and a characteristic pair of direct repeat sequences of 15 bp each: one is situated in the right extremity ofmec DNA, and the other is situated outside themec DNA and abuts the left boundary of mec DNA. The integration site of mec DNA was found to be located in an open reading frame (ORF) of unknown function, designatedorfX. Clusters of antibiotic resistance genes were noted inmec DNA carried by transposon Tn554 and an integrated copy of plasmid pUB110. Both the transposon and plasmid were integrated in the proximity of the mecA gene, the latter being flanked by a pair of insertion sequence IS431elements. Many ORFs other than those encoding antibiotic resistance were considered nonfunctional because of the acquired mutations or partial deletions found in the ORFs. Two ORFs potentially encoding novel site-specific recombinases were found in mec DNA. However, there was no ORF that might encode mecDNA-specific transposase or integrase proteins, indicating that themec DNA is not a transposon or a bacteriophage in nature.

scholarly journals Investigation of antibiotic resistance pattern of Staphylococcus aureus in clinical samples of animals and humans from selective areas of Bangladesh

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
M. A. Islam ◽  
M. S. Uddin ◽  
M. J. Islam ◽  
M. U. Ahmed ◽  
M. M. Alam
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Fusidic Acid ◽  
Animal Origin ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Antibiotic Resistance Pattern ◽  
Potential Pathogen

Background: Staphylococcus aureus (S. aureus) is a potential pathogen responsible for producing various infectious diseases. Determination of the prevalence of S. aureus infections both in animals and humans and elucidation of their antibiotic resistance pattern is crucial. The objective of this study was to find out the prevalence of Staphylococcal diseases in animals and humans, and their resistance pattern to commonly used antibiotics. Methods: A total of 100 animal and 100 human clinical samples were analyzed by traditional method. The S. aureus was identified by their cultural characteristics, gram’s staining, and catalase and coagulase tests. Antibiotic resistance pattern of the isolates was determined by disc diffusion method using various types of antibiotics. Results: The prevalence of S. aureus in animals and humans were 54% and 40%, respectively. S. aureus isolates of animal origin were highly resistant against penicillin (64.81%) and oxytetracycline (42.59%), and the lowest resistance was against oxacillin (7.40 %). S. aureus isolates from human were also showed a higher percentage of resistance against penicillin (87.5%) then oxacillin, cloxacillin, amoxicillin (37.5% each), and lowest resistance was observed against fusidic acid (5%). Conclusion: The findings of this study will certainly help veterinary clinicians or physicians to select appropriate antibiotics like oxacillin and fusidic acid for the treatment of different types of staphylococcal infections.

scholarly journals Prevalence of Nasal Carriers of Staphylococcus aureus and Determination of Antibiotic Resistance in Hemodialysis Patient in Kamkar Hospital in Qom City, (Iran)

2018 ◽  
Vol 12 (3) ◽  
pp. 66-75
Author(s):  
Shykholeslsm Nazanin ◽  
Halimeh Ejraii ◽  
Kiana Vafaei ◽  
Shahram Arsang ◽  
Hosein Saghafi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Hemodialysis Patient

Rapid detection of trace Cu 2+ using an l ‐cysteine based interdigitated electrode sensor integrated with AC electrokinetic enrichment

2019 ◽  
Vol 40 (20) ◽  
pp. 2699-2705 ◽  
Author(s):  
Haochen Qi ◽  
Meiqi Zhao ◽  
Huaguo Liang ◽  
Jayne Wu ◽  
Zhengfeng Huang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Interdigitated Electrode ◽  
Electrode Sensor
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