Determination of nanoparticle localisation within subcellular organelles in vitro using Raman spectroscopy

Raman spectroscopy with the aid of Multivariate Analysis techniques is a powerful analytical tool to determine the localisation of nanoparticles and their local environment within subcellular organelles.

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In vitro monitoring of time and dose dependent cytotoxicity of aminated nanoparticles using Raman spectroscopy

The Analyst ◽

10.1039/c6an01199c ◽

2016 ◽

Vol 141 (18) ◽

pp. 5417-5431 ◽

Cited By ~ 19

Author(s):

Esen Efeoglu ◽

Alan Casey ◽

Hugh J. Byrne

Keyword(s):

Raman Spectroscopy ◽

Multivariate Analysis ◽

Cellular Toxicity ◽

Molecular Changes ◽

Analysis Techniques ◽

Raman Spectral ◽

Dose Dependent

Monitoring of time and dose dependent molecular changes by using Raman spectroscopy with the aid of multivariate analysis techniques and determination of Raman spectral markers of cellular toxicity.

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Precise Determination of Percent Cure of Epoxide Polymers and Composites via Fiber-Optic Raman Spectroscopy and Multivariate Analysis

Applied Spectroscopy ◽

10.1366/0003702971939956 ◽

1997 ◽

Vol 51 (2) ◽

pp. 247-252 ◽

Cited By ~ 31

Author(s):

Jeffrey F. Aust ◽

Karl S. Booksh ◽

Christopher M. Stellman ◽

Richard S. Parnas ◽

Michael L. Myrick

Keyword(s):

Raman Spectroscopy ◽

Multivariate Analysis ◽

Real Time ◽

Fiber Optic ◽

Precise Determination ◽

Probe Design ◽

Epoxide Polymers ◽

Time Determination

A method for real-time determination of the percent cure of epoxies via in situ fiber-optic Raman spectroscopy has been developed. This method utilizes a probe design developed for real-time monitoring of polymer curing and multivariate analysis to interpret the data and determine percent cure. This method was demonstrated to be reliable to ±0.54% of cure in laboratory samples over a 50–99% cure range. A preliminary study measuring cure percentage in an industrial, glass-reinforced composite has been shown to be reliable to ±0.82% in the 40–90% cure range.

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Determination of carbaryl pesticide in Fuji apples using surface-enhanced Raman spectroscopy coupled with multivariate analysis

LWT ◽

10.1016/j.lwt.2014.08.011 ◽

2015 ◽

Vol 60 (1) ◽

pp. 352-357 ◽

Cited By ~ 59

Author(s):

Yuxia Fan ◽

Keqiang Lai ◽

Barbara A. Rasco ◽

Yiqun Huang

Keyword(s):

Raman Spectroscopy ◽

Multivariate Analysis ◽

Surface Enhanced Raman Spectroscopy ◽

Surface Enhanced ◽

Surface Enhanced Raman

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Surprising S1-resistant trimolecular hybrids: potential complication in interpretation of S1 mapping analyses

Molecular and Cellular Biology ◽

10.1128/mcb.5.10.2842-2846.1985 ◽

1985 ◽

Vol 5 (10) ◽

pp. 2842-2846

Author(s):

M A Lopata ◽

B Sollner-Webb ◽

D W Cleveland

Keyword(s):

Distal Region ◽

Analytical Tool ◽

Dna Probe ◽

Dna Transfection ◽

Potential Complication ◽

Animal Cells ◽

Rna Molecules ◽

S1 Mapping ◽

Powerful Analytical Tool

Although the technique of S1 mapping is a powerful analytical tool for the analysis of RNA, we now report a surprising complication involving a trimolecular hybrid between two RNA species and a single DNA probe molecule which, if unrecognized, can lead to misleading interpretations. We document that such trimolecular hybrids can be efficiently formed under some hybridization conditions and that the probe DNA sequence at the junction of the two RNA molecules can be remarkably stable to digestion with S1. Trimolecular hybrids can arise in any instance whenever a distal region of an end-labeled DNA probe is homologous to a moderately abundant RNA in the sample to be analyzed. This situation presents a serious, potential complication for a variety of S1 analyses, particularly those in which DNA transfection has been utilized to reintroduce in vitro-engineered genes into cultured animal cells.

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