Turn-on and label-free fluorescence detection of lead ions based on target-induced G-quadruplex formation

2015 ◽  
Vol 51 (38) ◽  
pp. 8165-8168 ◽  
Author(s):  
Lijun Xu ◽  
Xiaoqiang Shen ◽  
Shanni Hong ◽  
Jine Wang ◽  
Yuanyuan Zhang ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
High Sensitivity ◽  
Lead Ions ◽  
Label Free ◽  
Low Background ◽  
Background Fluorescence ◽  
Turn On ◽  
Sensing Systems ◽  
G Quadruplex ◽  
Quadruplex Formation

Using a guanine-rich sequence (AGRO100) and N-methyl mesoporphyrin IX (NMM), a turn-on and label-free fluorescent Pb2+ sensor with high sensitivity and low background fluorescence was presented as a representative of five turn-on sensing systems.

Label-free fluorescence turn-on detection of uracil DNA glycosylase activity based on G-quadruplex formation

Talanta ◽  
2016 ◽  
Vol 160 ◽  
pp. 449-453 ◽  
Author(s):  
Changbei Ma ◽  
Kefeng Wu ◽  
Haisheng Liu ◽  
Kun Xia ◽  
Kemin Wang ◽  
...  
Keyword(s):  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Turn On ◽  
G Quadruplex ◽  
Quadruplex Formation ◽  
Fluorescence Turn On

A Highly Sensitive and Label-Free Microbead-Based ‘Turn-On’ Colorimetric Sensor for the Detection of Mercury(II) in Urine Using a Peroxidase-Like Split G-Quadruplex–Hemin DNAzyme

2018 ◽  
Vol 71 (12) ◽  
pp. 945
Author(s):  
Xin Fu ◽  
He Zhang ◽  
Jie Zhang ◽  
Shi-Tong Wen ◽  
Xing-Cheng Deng
Keyword(s):  
Standard Deviation ◽  
Base Pair ◽  
Limit Of Detection ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Relative Standard ◽  
G Quadruplex ◽  
Catalytically Active ◽  
Partial Cdna

A highly sensitive and label-free microbead-based ‘turn-on’ assay was developed for the detection of Hg2+ in urine based on the Hg2+-mediated formation of intermolecular split G-quadruplex–hemin DNAzymes. In the presence of Hg2+, T–T mismatches between the two partial cDNA strands were stabilized by a T–Hg2+–T base pair, and can cause the G-rich sequences of the two oligonucleotides to associate to form a split G-quadruplex which is able to bind hemin to form the catalytically active G-quadruplex–hemin DNAzyme. This microbead-based ‘turn-on’ process allows the detection of Hg2+ in urine samples at concentrations as low as 0.5 pM. The relative standard deviation and recovery are 1.2–3.9 and 98.7–103.2%, respectively. The remarkable sensitivity for Hg2+ is mainly attributed to the enhanced mass transport ability that is inherent in homogeneous microbead-based assays. Compared with previous developments of intermolecular split G-quardruplex–hemin DNAzymes for the homogeneous detection of Hg2+ (the limit of detection was 19nM), a signal enhancement of ~1000 times is obtained when such an assay is performed on the surface of microbeads.

Label-free and sensitive detection of coralyne and heparin based on target-induced G-quadruplex formation

2019 ◽  
Vol 11 (10) ◽  
pp. 1331-1337 ◽  
Author(s):  
Mingjian Chen ◽  
Changbei Ma ◽  
Han Zhao ◽  
Kemin Wang
Keyword(s):  
Detection Method ◽  
Sensitive Detection ◽  
Label Free ◽  
G Quadruplex ◽  
Quadruplex Formation

Herein we propose a label-free and sensitive detection method for coralyne and heparin, based on utilizing the complex of adenosine16 (A16) and coralyne to induce the formation of a G-quadruplex scaffold.

scholarly journals Design of a Fluorescence Turn-on and Label-free Aptasensor Using the Intrinsic Quenching Power of G-Quadruplex to AMT

2020 ◽  
Vol 36 (8) ◽  
pp. 965-970
Author(s):  
Dandan WANG ◽  
Fenghua GENG ◽  
Yongxiang WANG ◽  
Yu MA ◽  
Guixin LI ◽  
...  
Keyword(s):  
Label Free ◽  
Turn On ◽  
G Quadruplex ◽  
Fluorescence Turn On

A “turn-on” and label-free fluorescent assay for the rapid detection of exonuclease III activity based on Tb3+-induced G-quadruplex conjugates

2014 ◽  
Vol 406 (18) ◽  
pp. 4535-4540 ◽  
Author(s):  
WeiJuan Yang ◽  
YaJuan Ruan ◽  
WeiHua Wu ◽  
PingPing Chen ◽  
LiangJun Xu ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Label Free ◽  
Fluorescent Assay ◽  
Exonuclease Iii ◽  
Turn On ◽  
G Quadruplex

Improved Detection of Hg2+ in Aqueous Solution with High Sensitivity and Selectivity by Using Mercury-Specific DNA, Sybr Green I and Gold Nanoparticles

2011 ◽  
Vol 239-242 ◽  
pp. 934-939
Author(s):  
Hui Xu ◽  
Shuli Gao ◽  
Jian Nong Chen ◽  
Quan Wen Liu
Keyword(s):  
Gold Nanoparticles ◽  
High Sensitivity ◽  
Sybr Green ◽  
Sybr Green I ◽  
Fluorescence Signal ◽  
Potassium Ions ◽  
Label Free ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
Fluorescence Turn On

We report a label-free, fast, fluorescence turn on assay for Hg2+detecton by using mercury-specific DNA (MSD), Sybr Green I (SG) and gold nanoparticles (AuNPs). SG efficiently discriminates MSD and MSD/Hg2+complex. The addition of gold nanoparticle decreases the background fluorescence signal further for MSD. The fluorescence intensity of MSD/Hg2+complex keeps constant after addition of AuNPs. This property improves the signal-to-background ratio and decreases the detection limitation further. In addition, the method shows improved selectivity compared with that in the absence of AuNPs. This strategy could be applied to the detection of potassium ions and showed good generality.

“Turn-on” Fluorescence Detection of Lead Ions Based on Accelerated Leaching of Gold Nanoparticles on the Surface of Graphene

2012 ◽  
Vol 4 (2) ◽  
pp. 1080-1086 ◽  
Author(s):  
Xiuli Fu ◽  
Tingting Lou ◽  
Zhaopeng Chen ◽  
Meng Lin ◽  
Weiwei Feng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Detection ◽  
Lead Ions ◽  
Turn On ◽  
Accelerated Leaching
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