Real-time in situ monitoring via europium emission of the photo-release of antitumor cisplatin from a Eu–Pt complex

2015 ◽  
Vol 51 (74) ◽  
pp. 14022-14025 ◽  
Author(s):  
Hongguang Li ◽  
Rongfeng Lan ◽  
Chi-Fai Chan ◽  
Lijun Jiang ◽  
Lixiong Dai ◽  
...  
Keyword(s):  
Real Time ◽  
Antitumor Agent ◽  
In Situ Monitoring ◽  
Two Photon ◽  
Turn On ◽  
Pt Complex ◽  
Photon Excitation ◽  
Two Photon Excitation

A light-responsive antitumor agent, PtEuL, has been synthesized and evaluated for controlled cisplatin release by linear/two-photon excitation in vitro with concomitant turn-on Eu emission as a responsive traceable signal.

Real-time monitoring of intracellular nitric oxide using a long-wavelength-emitting probe via one-photon or two-photon excitation

2019 ◽  
Vol 7 (11) ◽  
pp. 3246-3252 ◽  
Author(s):  
Changquan Tang ◽  
Mingxue Wang ◽  
Xiaoying Shang ◽  
Xueyuan Chen ◽  
Deguang Huang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Long Wavelength ◽  
Two Photon ◽  
Turn On ◽  
Photon Excitation ◽  
Endogenous Nitric Oxide ◽  
Two Photon Excitation

A long-wavelength-emitting fluorescent turn-on probe is developed for monitoring exogenous or endogenous nitric oxide in living cells.

Determination of absolute two-photon excitation cross sections by in situ second-order autocorrelation

1995 ◽  
Vol 20 (23) ◽  
pp. 2372 ◽  
Author(s):  
Chris Xu ◽  
Winfried Denk ◽  
Jeffrey Guild ◽  
Watt W. Webb
Keyword(s):  
Cross Sections ◽  
Second Order ◽  
Two Photon ◽  
Photon Excitation ◽  
Excitation Cross Sections ◽  
Two Photon Excitation

Two-Photon Excitation of Potentiometric Probes Enables Optical Recording of Action Potentials From Mammalian Nerve Terminals In Situ

2008 ◽  
Vol 99 (3) ◽  
pp. 1545-1553 ◽  
Author(s):  
Jonathan A. N. Fisher ◽  
Jonathan R. Barchi ◽  
Cristin G. Welle ◽  
Gi-Ho Kim ◽  
Paul Kosterin ◽  
...  
Keyword(s):  
Action Potentials ◽  
Optical Recording ◽  
Photon Absorption ◽  
Nerve Terminals ◽  
Electrode Arrays ◽  
Two Photon ◽  
Photon Excitation ◽  
Mammalian Nerve ◽  
Two Photon Excitation

We report the first optical recordings of action potentials, in single trials, from one or a few (∼1–2 μm) mammalian nerve terminals in an intact in vitro preparation, the mouse neurohypophysis. The measurements used two-photon excitation along the “blue” edge of the two-photon absorption spectrum of di-3-ANEPPDHQ (a fluorescent voltage-sensitive naphthyl styryl-pyridinium dye), and epifluorescence detection, a configuration that is critical for noninvasive recording of electrical activity from intact brains. Single-trial recordings of action potentials exhibited signal-to-noise ratios of ∼5:1 and fractional fluorescence changes of up to ∼10%. This method, by virtue of its optical sectioning capability, deep tissue penetration, and efficient epifluorescence detection, offers clear advantages over linear, as well as other nonlinear optical techniques used to monitor voltage changes in localized neuronal regions, and provides an alternative to invasive electrode arrays for studying neuronal systems in vivo.

scholarly journals Interactions of elastic and rigid vesicles with human skin in vitro: electron microscopy and two-photon excitation microscopy

1999 ◽  
Vol 1461 (1) ◽  
pp. 155-173 ◽  
Author(s):  
Benedicte A.I. van den Bergh ◽  
Jurrien Vroom ◽  
Hans Gerritsen ◽  
Hans E. Junginger ◽  
Joke A. Bouwstra
Keyword(s):  
Electron Microscopy ◽  
Human Skin ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Dynamic erectile responses of a novel penile organ model utilizing two photon excitation microscopy (TPEM)†

2021 ◽  
Author(s):  
Daiki Hashimoto ◽  
Tsuyoshi Hirashima ◽  
Hisao Yamamura ◽  
Tomoya Kataoka ◽  
Kota Fujimoto ◽  
...  
Keyword(s):  
Structural Changes ◽  
Corpus Cavernosum ◽  
Oxidative Stress Marker ◽  
Relaxation Processes ◽  
No Donor ◽  
Two Photon ◽  
Photon Excitation ◽  
Sinusoidal Space ◽  
Two Photon Excitation

Abstract Male penis is required to become erect during copulation. In the upper (dorsal) part of penis, the erectile tissue termed corpus cavernosum (CC) plays fundamental roles for erection by regulating the inner blood flow. When blood flows into the CC, the microvascular complex termed sinusoidal space is reported to expand during erection. A novel in vitro explant system to analyze the dynamic erectile responses during contraction/relaxation is established. The current data show regulatory contraction/relaxation processes induced by phenylephrine (PE) and nitric oxide (NO) donor mimicking dynamic erectile responses by in vitro CC explants. Two photon excitation microscopy (TPEM) observation shows the synchronous movement of sinusoidal space and the entire CC. By taking advantages of the CC explant system, tadalafil (cialis) was shown to increase sinusoidal relaxation. Histopathological changes have been generally reported associating with erection in several pathological conditions. Various stressed statuses have been suggested to occur in the erectile responses by previous studies. The current CC explant model enables to analyze such conditions through directly manipulating CC in the repeated contraction/relaxation processes. Expression of oxidative stress marker and contraction related genes, Hif1a, Gpx1, RhoA, Rock was significantly increased in such repeated contraction/relaxation. Altogether, it is suggested that the system is valuable for analyzing structural changes and physiological responses to several regulators in the field of penile medicine.

A two-photon fluorescence self-reporting black phosphorus nanoprobe for the in situ monitoring of therapy response

2020 ◽  
Vol 56 (90) ◽  
pp. 14007-14010
Author(s):  
Kesong Guan ◽  
Peng Wang ◽  
Fang Zhou ◽  
Youjuan Wang ◽  
Hong-Wen Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Therapeutic Response ◽  
Therapy Response ◽  
Black Phosphorus ◽  
In Situ Monitoring ◽  
Two Photon ◽  
Fluorescent Nanoprobe ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We developed a black phosphorus based two-photon fluorescent nanoprobe (TPBP) for the in situ and real-time reporting of the therapeutic response of black phosphorus.

scholarly journals Two-Photon Excitation of Azobenzene Photoswitches for Synthetic Optogenetics

2020 ◽  
Vol 10 (3) ◽  
pp. 805 ◽  
Author(s):  
Shai Kellner ◽  
Shai Berlin
Keyword(s):  
Visible Light ◽  
Light Illumination ◽  
Optical Technique ◽  
Tissue Penetration ◽  
Cellular Targets ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Synthetic optogenetics is an emerging optical technique that enables users to photocontrol molecules, proteins, and cells in vitro and in vivo. This is achieved by use of synthetic chromophores—denoted photoswitches—that undergo light-dependent changes (e.g., isomerization), which are meticulously designed to interact with unique cellular targets, notably proteins. Following light illumination, the changes adopted by photoswitches are harnessed to affect the function of nearby proteins. In most instances, photoswitches absorb visible light, wavelengths of poor tissue penetration, and excessive scatter. These shortcomings impede their use in vivo. To overcome these challenges, photoswitches of red-shifted absorbance have been developed. Notably, this shift in absorbance also increases their compatibility with two-photon excitation (2PE) methods. Here, we provide an overview of recent efforts devoted towards optimizing azobenzene-based photoswitches for 2PE and their current applications.

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