Molecular interactions between gold nanoparticles and model cell membranes

2015 ◽  
Vol 17 (15) ◽  
pp. 9873-9884 ◽  
Author(s):  
Peipei Hu ◽  
Xiaoxian Zhang ◽  
Chi Zhang ◽  
Zhan Chen
Keyword(s):  
Gold Nanoparticles ◽  
Cell Membrane ◽  
Mammalian Cell ◽  
Molecular Interactions ◽  
Cell Membranes ◽  
Membrane Interactions ◽  
Flip Flop ◽  
Direct Observations ◽  
Model Cell

Direct observations of the lipid flip-flop induced by Au NP – model mammalian cell membrane interactions.

Elucidation of molecular interactions between lipid membranes and ionic liquids using model cell membranes

Soft Matter ◽  
2012 ◽  
Vol 8 (20) ◽  
pp. 5501 ◽  
Author(s):  
Seunghwan Jeong ◽  
Sung Ho Ha ◽  
Sang-Hyun Han ◽  
Min-Cheol Lim ◽  
Sun Min Kim ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Molecular Interactions ◽  
Lipid Membranes ◽  
Cell Membranes ◽  
Model Cell

Molecular Interactions Between Silver Nanoparticles and Model Cell Membranes

2018 ◽  
Vol 61 (9-11) ◽  
pp. 1148-1162 ◽  
Author(s):  
Peipei Hu ◽  
Xiaoxian Zhang ◽  
Yaoxin Li ◽  
Cayla Pichan ◽  
Zhan Chen
Keyword(s):  
Silver Nanoparticles ◽  
Molecular Interactions ◽  
Cell Membranes ◽  
Model Cell

Distribution of Functionalized Gold Nanoparticles between Water and Lipid Bilayers as Model Cell Membranes

2012 ◽  
Vol 46 (3) ◽  
pp. 1869-1876 ◽  
Author(s):  
Wen-Che Hou ◽  
Babak Yaghoubi Moghadam ◽  
Charlie Corredor ◽  
Paul Westerhoff ◽  
Jonathan D. Posner
Keyword(s):  
Gold Nanoparticles ◽  
Lipid Bilayers ◽  
Cell Membranes ◽  
Functionalized Gold Nanoparticles ◽  
Model Cell

Molecular Interactions between Amantadine and Model Cell Membranes

Langmuir ◽  
2014 ◽  
Vol 30 (28) ◽  
pp. 8491-8499 ◽  
Author(s):  
Fu-Gen Wu ◽  
Pei Yang ◽  
Chi Zhang ◽  
Bolin Li ◽  
Xiaofeng Han ◽  
...  
Keyword(s):  
Molecular Interactions ◽  
Cell Membranes ◽  
Model Cell

scholarly journals Surfactants, Salt, and pH Alter Nanoparticle-Model Cell Membrane Interactions

2014 ◽  
Vol 106 (2) ◽  
pp. 286a
Author(s):  
Katie Brennan ◽  
Luke Cuculis ◽  
Shelli L. Frey
Keyword(s):  
Cell Membrane ◽  
Membrane Interactions ◽  
Model Cell ◽  
Model Cell Membrane

scholarly journals A small molecule that mitigates bacterial infection disrupts Gram-negative cell membranes and is inhibited by cholesterol and neutral lipids

2020 ◽  
Vol 16 (12) ◽  
pp. e1009119
Author(s):  
Jamie L. Dombach ◽  
Joaquin L. J. Quintana ◽  
Toni A. Nagy ◽  
Chun Wan ◽  
Amy L. Crooks ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Cell Membrane ◽  
Mammalian Cell ◽  
Small Molecule ◽  
Cell Membranes ◽  
Neutral Lipids ◽  
Intraperitoneal Administration ◽  
Membrane Integrity ◽  
Gram Negative Bacteria ◽  
Gram Negative

Infections caused by Gram-negative bacteria are difficult to fight because these pathogens exclude or expel many clinical antibiotics and host defense molecules. However, mammals have evolved a substantial immune arsenal that weakens pathogen defenses, suggesting the feasibility of developing therapies that work in concert with innate immunity to kill Gram-negative bacteria. Using chemical genetics, we recently identified a small molecule, JD1, that kills Salmonella enterica serovar Typhimurium (S. Typhimurium) residing within macrophages. JD1 is not antibacterial in standard microbiological media, but rapidly inhibits growth and curtails bacterial survival under broth conditions that compromise the outer membrane or reduce efflux pump activity. Using a combination of cellular indicators and super resolution microscopy, we found that JD1 damaged bacterial cytoplasmic membranes by increasing fluidity, disrupting barrier function, and causing the formation of membrane distortions. We quantified macrophage cell membrane integrity and mitochondrial membrane potential and found that disruption of eukaryotic cell membranes required approximately 30-fold more JD1 than was needed to kill bacteria in macrophages. Moreover, JD1 preferentially damaged liposomes with compositions similar to E. coli inner membranes versus mammalian cell membranes. Cholesterol, a component of mammalian cell membranes, was protective in the presence of neutral lipids. In mice, intraperitoneal administration of JD1 reduced tissue colonization by S. Typhimurium. These observations indicate that during infection, JD1 gains access to and disrupts the cytoplasmic membrane of Gram-negative bacteria, and that neutral lipids and cholesterol protect mammalian membranes from JD1-mediated damage. Thus, it may be possible to develop therapeutics that exploit host innate immunity to gain access to Gram-negative bacteria and then preferentially damage the bacterial cell membrane over host membranes.

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