Intra-bundle contractions enable extensile properties of active actin networks

The cellular cortex is a dynamic and contractile actomyosin network modulated by actin-binding proteins. We reconstituted a minimal cortex adhered to a model cell membrane mimicking two processes mediated by the motor protein myosin: contractility and high turnover of actin monomers. Myosin reorganized these networks by extensile intra‑bundle contractions leading to an altered growth mechanism. Hereby, stress within tethered bundles induced nicking of filaments followed by repair via incorporation of free monomers. This mechanism was able to break the symmetry of the previously disordered network resulting in the generation of extensile clusters, reminiscent of structures found within cells.

Direct translocation of a negatively charged nanoparticle across a negatively charged model cell membrane

Nanoparticles have attracted much attention as a carrier for drug, gene, and macromolecule delivery in next-generation biomedical and therapeutic technologies. In delivery applications, nanoparticles tend to have negative charge due...

Iron-mediated interaction of alpha synuclein with lipid raft model membranes

We demonstrated that pathological conditions as accumulation of iron cations promote fast formation of α-synuclein aggregation in vitro, which preferentially interact with lipid-raft domains in model cell membrane systems.

Direct translocation of nanoparticles across a model cell membrane by nanoparticle-induced local enhancement of membrane potential

Nanoparticles directly translocate across a cell membrane by a locally enhanced membrane potential at the NP/cell-membrane contact interface.

A New Type of LSPR Sensor Featuring Immobilized Liposome or Phospholipid Single Layer

We have fabricated a new type of LSPR sensor featuring immobilized liposome or phospholipid single layer. LSPR principally shows an ultrahigh sensitivity on surface dielectric environmental change due to interaction with target, but little has been reported so far on applying phospholipid membranes and/or liposomes as model cell membrane. We newly tried to investigate biosensing capabilities using the membranes of the both structures on Au nanostructures of LSPR sensor chip. As a result, it was confirmed that the phospholipid single layer is more effective to improve the sensitivity than the liposome. Finally, we have clearly detected 100 nM target protein of CAB and estimated a possible detection of 10 nM range from wavelength resolution by interaction with the phospholipid single layer.