scholarly journals LabDisk for SAXS: a centrifugal microfluidic sample preparation platform for small-angle X-ray scattering

Lab on a Chip ◽  
2016 ◽  
Vol 16 (7) ◽  
pp. 1161-1170 ◽  
Author(s):  
Frank Schwemmer ◽  
Clement E. Blanchet ◽  
Alessandro Spilotros ◽  
Dominique Kosse ◽  
Steffen Zehnle ◽  
...  
Keyword(s):  
Protein Structure ◽  
Sample Preparation ◽  
Structure Analysis ◽  
Small Angle ◽  
Protein Structure Analysis ◽  
X Ray ◽  
X Ray Scattering ◽  
Synchrotron Beamlines ◽  
Ray Scattering

We present a centrifugal microfluidic LabDisk for protein structure analysis via small-angle X-ray scattering (SAXS) on synchrotron beamlines.

Influence of the sample preparation method of the ultra-small-angle x-ray scattering of lightly sulfonated polystyrenes

1993 ◽  
Vol 26 (15) ◽  
pp. 4064-4066 ◽  
Author(s):  
B. P. Grady ◽  
H. Matsuoka ◽  
Y. Nakatani ◽  
S. L. Cooper ◽  
N. Ise
Keyword(s):  
Sample Preparation ◽  
Small Angle ◽  
Preparation Method ◽  
Sample Preparation Method ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

Nanopore Structure Analysis of Deformed Coal from Nitrogen Isotherms and Synchrotron Small Angle X-ray Scattering

2017 ◽  
Vol 17 (9) ◽  
pp. 6224-6234 ◽  
Author(s):  
Xiaoshi Li ◽  
Yiwen Ju ◽  
Quanlin Hou ◽  
Zhuo Li ◽  
Qiangguang Li ◽  
...  
Keyword(s):  
Structure Analysis ◽  
Small Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Nanopore Structure ◽  
Ray Scattering

Silver behenate as a calibration standard of grazing-incidence small-angle X-ray scattering

2006 ◽  
Vol 39 (5) ◽  
pp. 749-751 ◽  
Author(s):  
Byeongdu Lee ◽  
Chieh-Tsung Lo ◽  
Soenke Seifert ◽  
Randall E. Winans
Keyword(s):  
Refractive Index ◽  
Sample Preparation ◽  
Small Angle ◽  
Incident Angle ◽  
Grazing Incidence ◽  
Peak Position ◽  
Calibration Standard ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

Grazing-incidence small-angle X-ray scattering (GISAXS) patterns of a silver behenate composite film, which has a typical layered structure, are described. The peak position of the film in the GISAXS pattern was varied depending on the incident angle, which was well described by taking into account the refraction and the reflection effects. Since the refractive index of samples depends on sample preparation, it is recommended that the measurement of silver behenate as a standard be done in conventional transmission mode to avoid any complexity.

scholarly journals Fine Structure Analysis of Ramie Fibres by Small Angle X-Ray Scattering Technique

1997 ◽  
Vol 29 (12) ◽  
pp. 1007-1011 ◽  
Author(s):  
T Misra ◽  
J Shukla ◽  
Md N Khan ◽  
D K Bisoyi ◽  
T Patel
Keyword(s):  
Fine Structure ◽  
Structure Analysis ◽  
Small Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Fine Structure Analysis ◽  
Scattering Technique ◽  
Ray Scattering

scholarly journals 1G1336 Measurement of Dynamic Fluctuation of Proteins using Small-Angle X-ray Scattering(Protein: Structure 1,The 49th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S45-S46
Author(s):  
Yasunobu Sugimoto ◽  
Takahiko Sugimoto ◽  
Yoshitaro Tanaka ◽  
Shinsaku Maruta ◽  
Jun Miyake
Keyword(s):  
Protein Structure ◽  
Annual Meeting ◽  
Small Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Biophysical Society ◽  
Dynamic Fluctuation ◽  
Ray Scattering

scholarly journals Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering

2011 ◽  
Vol 44 (5) ◽  
pp. 1090-1099 ◽  
Author(s):  
Josiane P. Lafleur ◽  
Detlef Snakenborg ◽  
Søren S. Nielsen ◽  
Magda Møller ◽  
Katrine N. Toft ◽  
...  
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Small Angle ◽  
Structural Investigation ◽  
Adaptor Protein ◽  
Complete Analysis ◽  
Uv Absorbance ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

A new microfluidic sample-preparation system is presented for the structural investigation of proteins using small-angle X-ray scattering (SAXS) at synchrotrons. The system includes hardware and software features for precise fluidic control, sample mixing by diffusion, automated X-ray exposure control, UV absorbance measurements and automated data analysis. As little as 15 µl of sample is required to perform a complete analysis cycle, including sample mixing, SAXS measurement, continuous UV absorbance measurements, and cleaning of the channels and X-ray cell with buffer. The complete analysis cycle can be performed in less than 3 min. Bovine serum albumin was used as a model protein to characterize the mixing efficiency and sample consumption of the system. The N2 fragment of an adaptor protein (p120-RasGAP) was used to demonstrate how the device can be used to survey the structural space of a protein by screening a wide set of conditions using high-throughput techniques.

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