Face the waves of emotion in the sea of the unconscious: Focusing on the symbolism of the sea and the waves

The purpose of this study was to examine the symbolism of the sea and waves, focusing on these symbols in the dreams and sand trays during the female client’s sandplay therapy. The client had dreamt about the sea repeatedly since childhood, so her repetitive dreams about the sea were analyzed during the sandplay sessions. She realized that the deep abyss of the sea was linked to her unconsciousness. Through the dynamic fluctuation of the waves, the emotions that she had suppressed became shaken, and it brought out her anger. As becoming aware of her suppressed emotions, she was able to heal herself. The repeated appearance of the sea in the sand trays connected her to the unconscious and led her to integration and wholeness. She made the choice of recharging her energy for love in the sea and returning to her life. The researcher could understand that it was the message of the sea to love herself and her life and live an authentic life of self-care proudly.

Glymphatic Dysfunction: A Bridge Between Sleep Disturbance and Mood Disorders

Mounting evidence demonstrates a close relationship between sleep disturbance and mood disorders, including major depression disorder (MDD) and bipolar disorder (BD). According to the classical two-process model of sleep regulation, circadian rhythms driven by the light–dark cycle, and sleep homeostasis modulated by the sleep–wake cycle are disrupted in mood disorders. However, the exact mechanism of interaction between sleep and mood disorders remains unclear. Recent discovery of the glymphatic system and its dynamic fluctuation with sleep provide a plausible explanation. The diurnal variation of the glymphatic circulation is dependent on the astrocytic activity and polarization of water channel protein aquaporin-4 (AQP4). Both animal and human studies have reported suppressed glymphatic transport, abnormal astrocytes, and depolarized AQP4 in mood disorders. In this study, the “glymphatic dysfunction” hypothesis which suggests that the dysfunctional glymphatic pathway serves as a bridge between sleep disturbance and mood disorders is proposed.

The Dynamic Fluctuation of Red Palm Weevil Rhynchophorus Ferrugineus (Olivier) in Makkah Al-Mukarramah city

The red palm weevil(RPW),Rhynchophorus ferrugineus (Olivier) (Coleoptera: Dryophthoridae) is one of the most threatening pests of date palm trees in Kingdom of Saudi Arabia (KSA). Which considered as category Iinsect pest in the Gulf region and the Middle East, according toFood and Agriculture Organization (FAO). Therefore, this research was planned to study the dynamic fluctuation of RPW, its relationship to some environmental factors (temperature & relative humidity) and to determine sexual ratio in Makkah city during 2019. In addition to clarifying color preference by using food bait pheromone traps (FBPTs). The results revealedthe presence of RPW throughout the year, with significant differences in numerical densities according to different collection times. The study also recorded the highest population density during April & March, and it was the highest significantly compared to that were collected during July, October, August and September. The study also recorded two peaks of RPW activity throughout the year. A major summit in April and a smaller summit in December. Statistical analysis results showed that the RPW has significant activity at spring compared to the other seasons, while the least active for RPW was at autumn.The results also showed a negative significant correlation between the mean population density of RPW and temperature; and a positive non-significant correlation between seasonal abundance and relative humidity, with significant differences between the mean of male and female with ratio(1:3), respectively. Black traps were more effective and significantly in attracting RPW than other tested colors.

Changes in tonsillolith characteristics detected in a follow-up CT study

Background Tonsilloliths are related clinically to halitosis and tonsillar abscess. However, the dynamics of tonsilloliths over time are unknown. The aim of the study was to evaluate change in the characteristics of tonsilloliths in a time-dependent fashion by follow-up computed tomography (CT). Methods Tonsilloliths were analyzed in 326 CT scan pair sets of initial and at least two follow-up CT examinations of patients with whole palatine tonsils and various diseases of the oral and maxillofacial regions. Results Over the follow-up period, 12.1% of tonsilloliths disappeared. Approximately 26.1% of tonsilloliths changed in size during follow-up, mostly increasing in size. In tonsilloliths that showed enlargement, the mean (± standard deviation) growth rate was 0.61 ± 0.41 mm per year. Approximately 37.3% of tonsilloliths changed position during the follow-up period; of these, movement was toward the respiratory tract in 92% at a mean rate of − 1.38 ± 1.59 mm per year. The calcification levels of almost all tonsilloliths showed dynamic change: HU number increased in 84.3% and decreased in 12.7% of tonsilloliths over the follow-up period. The mean rate of HU increase was 63.8 ± 96.3 HU/year, and the mean rate of HU decrease was − 38.4 ± 66.8 HU/year. Conclusions The calcification levels of all tonsilloliths showed dynamic fluctuation, and a tendency for excretion of tonsilloliths from the body. Their dynamics over time suggest that tonsilloliths may be in a permanently active phase which functions to remove foreign matter.

Nucleocapsid protein preferentially binds the stem-loop of duplex/quadruplex hybrid that unfolds the quadruplex structure

HIV nucleocapsid protein unfolds the duplex/quadruplex hybrid structure by binding to the stem-loop region, which induces dynamic fluctuation of G-tetrad and K+ ejection.

Properties and biological impact of RNA G-quadruplexes: from order to turmoil and back

Guanine-quadruplexes (G4s) are non-canonical four-stranded structures that can be formed in guanine (G) rich nucleic acid sequences. A great number of G-rich sequences capable of forming G4 structures have been described based on in vitro analysis, and evidence supporting their formation in live cells continues to accumulate. While formation of DNA G4s (dG4s) within chromatin in vivo has been supported by different chemical, imaging and genomic approaches, formation of RNA G4s (rG4s) in vivo remains a matter of discussion. Recent data support the dynamic nature of G4 formation in the transcriptome. Such dynamic fluctuation of rG4 folding-unfolding underpins the biological significance of these structures in the regulation of RNA metabolism. Moreover, rG4-mediated functions may ultimately be connected to mechanisms underlying disease pathologies and, potentially, provide novel options for therapeutics. In this framework, we will review the landscape of rG4s within the transcriptome, focus on their potential impact on biological processes, and consider an emerging connection of these functions in human health and disease.

Experimental Reproduction of Dynamic Fluctuation of TSH Receptor–Binding Antibodies Between Stimulation and Inhibition

Context Hyperthyroidism in Graves disease (GD) is caused by autoantibody stimulation of the TSH receptor (TSHR). TSHR autoantibody (TSHR-Ab) activity is measured routinely by inhibition of labeled ligand (TSH or M22) binding to the TSHR [TSH-binding inhibitory immunoglobulins (TBIIs)] or by stimulation of cAMP production in isolated cells [TSH receptor–stimulating antibodies (TSAbs)]. Usually, measurements of TSHR-Abs by TBIIs agree reasonably well with TSAb values at least in the setting of hyperthyroidism, and both measurements tend to change in parallel during treatment with some exceptions. In this study, we describe three unusual cases, which illustrate nearly pure stimulating, blocking, or neutral properties of TSHR-Abs. Objective Whether patient serum TSHR-Abs can be reproduced by mixtures of human monoclonal autoantibodies to the TSHR was studied because the sera in most patients show moderate properties having both of TBII and TSAb activities. Design We compared the TBII and TSAb activities of serum from four unusual patients in detail with mixtures of human monoclonal TSHR-Abs (mAbs) M22 (stimulating), K1-18 (stimulating), and K1-70 (blocking). Results Characteristic of a patient’s serum was similar to M22 or K1-18, another was similar to K1-70, whereas another was similar to a mixture of K1-70 and M22 (or K1-18). Additionally, some patients seemed to have neutral TSHR-Abs in their sera. Conclusions Our studies suggest that the characteristics of TSHR-Abs in the patient serum can be mimicked by mixtures of human mAbs to the TSHR, stimulating, blocking, and neutral if any.