Development of pipette tip-based on molecularly imprinted polymer micro-solid phase extraction for selective enantioselective determination of (−)-(2S,4R) and (+)-(2R,4S) ketoconazole in human urine samples prior to HPLC-DAD

2016 ◽  
Vol 8 (20) ◽  
pp. 4075-4085 ◽  
Author(s):  
Ricky Cássio Santos da Silva ◽  
Valdir Mano ◽  
Arnaldo César Pereira ◽  
Eduardo Costa de Figueiredo ◽  
Keyller Bastos Borges
Keyword(s):  
Human Urine ◽  
Solid Phase ◽  
Urine Samples ◽  
Preparation Technique ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Human Urine Samples ◽  
Sample Preparation Technique ◽  
Pipette Tip

A simple and selective sample preparation technique employing PT-MIP-μ-SPE coupled to HPLC/DAD was developed for the determination of the cis-enantiomers of ketoconazole in human urine samples.

Microextraction by packed sorbent using a new restricted molecularly imprinted polymer for the determination of estrogens from human urine samples

2019 ◽  
Vol 150 ◽  
pp. 104162 ◽  
Author(s):  
Hanna Leijoto de Oliveira ◽  
Leila Suleimara Teixeira ◽  
Laíse Aparecida Fonseca Dinali ◽  
Bruna Carneiro Pires ◽  
Nathália Soares Simões ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Urine Samples ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Human Urine Samples ◽  
Microextraction By Packed Sorbent ◽  
Packed Sorbent

Efficient molecularly imprinted polymer as a pipette-tip solid-phase sorbent for determination of carvedilol enantiomers in human urine

2017 ◽  
Vol 1061-1062 ◽  
pp. 399-410 ◽  
Author(s):  
Anny Talita Maria da Silva ◽  
Hanna Leijoto de Oliveira ◽  
Camilla Fonseca Silva ◽  
Matheus Cecílio Fonseca ◽  
Thales Fernando Dias Pereira ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Solid Phase ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Pipette Tip

scholarly journals Assessment of the Performance of Solid Phase Extraction Based on Pipette Tip Employing a Hybrid Molecularly Imprinted Polymer as an Adsorbent for Enantioselective Determination of Albendazole Sulfoxide

2019 ◽  
Vol 57 (7) ◽  
pp. 671-678 ◽  
Author(s):  
Sara da Silva Anacleto ◽  
Hanna Leijoto de Oliveira ◽  
Anny Talita Maria da Silva ◽  
Tienne Aparecida do Nascimento ◽  
Marcella Matos Cordeiro Borges ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Albendazole Sulfoxide ◽  
Pipette Tip

Abstract Herein, an organic–inorganic hybrid molecularly imprinted polymer (MIP) was successfully synthesized with albendazole sulfoxide (ABZSO) as a template and 3-(trimethoxysilyl)propyl methacrylate, a bifunctional group compound, as a single cross-linking agent. In this study, a simple method using HPLC–DAD was developed for the determination of ABZSO enantiomers in human urine using pipette tip-based molecularly imprinted polymer solid phase extraction (PT–MIP–SPE). Enantioseparation with satisfactory retention times (5.17 and 7.09 min), acceptable theoretical plates (N = 4,535 and 5,091) and strong resolution (Rs = 5.45) was performed with an Agilent® Eclipse Plus C18 (100 mm × 4.6 mm, 3.5 μm) coupled with a Chiralpak® IA column (100 mm × 4.6 mm, 3 μm), a mixture with ethanol:water (50:50, v/v) as the mobile phase, temperature at 40°C, flow rate at 0.9 mL min−1 and λ = 230 nm. Thereafter, certain parameters affecting the PT–MIP–SPE were investigated in detail and the better conditions were: 300 μL of water as washing solvent, 500 μL of ethanol:acetic acid (9:1, v/v) as eluting solvent, 20 mg of MIP, 500 μL of human urine at pH 9 and no addition of NaCl. Recoveries/relative standard deviation (RSD%) for (R)-(+)-ABZSO and (S)-(−)-ABZSO were 78.2 ± 0.2% and 69.7 ± 1.7%, respectively.

Hollow mesoporous structured molecularly imprinted polymer as adsorbent in pipette-tip solid-phase extraction for the determination of antiretrovirals from plasma of HIV-infected patients

2018 ◽  
Vol 39 (20) ◽  
pp. 2581-2589 ◽  
Author(s):  
Nathália Soares Simões ◽  
Hanna Leijoto de Oliveira ◽  
Ricky Cássio Santos da Silva ◽  
Leila Suleimara Teixeira ◽  
Thaís Lorenna Souza Sales ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymer ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Pipette Tip
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