Hairpin DNA-fueled dynamic self-assembly of three-arm DNA branched junctions consisting of active DNAzyme structures for enzyme-free ultrasensitive detection of nucleic acids

2016 ◽  
Vol 8 (47) ◽  
pp. 8262-8265 ◽  
Author(s):  
Ke Yang ◽  
Ming Zeng ◽  
Xing He ◽  
Jianming Li ◽  
Dinggeng He
Keyword(s):  
Nucleic Acids ◽  
Self Assembly ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Hairpin Dna ◽  
Amplification Strategy ◽  
Dual Signal Amplification

An enzyme-free dual signal amplification strategy based on programmable molecular hairpins has been developed for amplified detection of DNA via the hairpin DNA-fueled dynamic self-assembly of three-arm DNAzyme.

The PCR-HCR dual signal amplification strategy for ultrasensitive detection of Escherichia coli O157:H7 in milk

LWT ◽  
2020 ◽  
Vol 130 ◽  
pp. 109642 ◽  
Author(s):  
Taobo Liang ◽  
Xin Wu ◽  
Bolu Chen ◽  
Ju Liu ◽  
Zoraida P. Aguilar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Escherichia Coli O157 ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A dual signal amplification strategy for the highly sensitive fluorescence detection of nucleic acids

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1219-1226 ◽  
Author(s):  
Jingjing Zhang ◽  
Chunyuan Song ◽  
Huiling Zhou ◽  
Juan Jia ◽  
Yinna Dai ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Detection ◽  
Signal Amplification ◽  
Fluorescence Assay ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A dual signal amplification strategy comprising target-triggered recycling and DSN-mediated amplifications was designed and proposed for a highly sensitive fluorescence assay of nucleic acids.

Biobar-Coded Gold Nanoparticles and DNAzyme-Based Dual Signal Amplification Strategy for Ultrasensitive Detection of Protein by Electrochemiluminescence

2014 ◽  
Vol 7 (1) ◽  
pp. 696-703 ◽  
Author(s):  
Hui Xia ◽  
Lingling Li ◽  
Zhouyang Yin ◽  
Xiandeng Hou ◽  
Jun-Jie Zhu
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Amplification Strategy ◽  
Dual Signal Amplification

scholarly journals Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification

ACS Omega ◽  
2020 ◽  
Vol 5 (50) ◽  
pp. 32738-32743
Author(s):  
Fubing Xiao ◽  
Jie Liu ◽  
Qinghui Guo ◽  
Zhibo Du ◽  
Hong Li ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Amplification Strategy ◽  
Dual Signal Amplification

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

A signal amplification strategy based on peptide self-assembly for the identification of amyloid-β oligomer

2021 ◽  
pp. 129697
Author(s):  
Yujin Huang ◽  
Baole Zhang ◽  
Liang Yuan ◽  
Lei Liu
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
Amyloid Β ◽  
Amplification Strategy

Dual-signal amplification strategy for electrochemiluminescence sandwich biosensor for detection of thrombin

2017 ◽  
Vol 240 ◽  
pp. 742-748 ◽  
Author(s):  
Yingjie Li ◽  
Yuqin Li ◽  
Ning Xu ◽  
Jiahong Pan ◽  
Tufeng Chen ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Amplification Strategy ◽  
Dual Signal Amplification
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