Revelation of molecular basis for chromium toxicity by phenotypes of Saccharomyces cerevisiae gene deletion mutants

Metallomics ◽  
2016 ◽  
Vol 8 (5) ◽  
pp. 542-550 ◽  
Author(s):  
Adam J. Johnson ◽  
Filip Veljanoski ◽  
Patrick. J. O'Doherty ◽  
Mohammad S. Zaman ◽  
Gayani Petersingham ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Basis ◽  
Gene Deletion ◽  
Deletion Mutants ◽  
Chromium Toxicity

Pleiotropic drug-resistance attenuated genomic library improves elucidation of drug mechanisms

2015 ◽  
Vol 11 (11) ◽  
pp. 3129-3136 ◽  
Author(s):  
Namal V. C. Coorey ◽  
James H. Matthews ◽  
David S. Bellows ◽  
Paul H. Atkinson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Mechanism Of Action ◽  
Gene Deletion ◽  
Genomic Library ◽  
Deletion Mutants ◽  
Pleiotropic Drug Resistance ◽  
Genome Wide

Identifying Saccharomyces cerevisiae genome-wide gene deletion mutants that confer hypersensitivity to a xenobiotic aids the elucidation of its mechanism of action (MoA).

Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures

2007 ◽  
Vol 2 (11) ◽  
pp. 2958-2974 ◽  
Author(s):  
Sarah E Pierce ◽  
Ron W Davis ◽  
Corey Nislow ◽  
Guri Giaever
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Deletion ◽  
Deletion Mutants ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Screening of High-level 4-Hydroxy-2(or 5)-Ethyl-5(or 2)-Methyl-3(2H)-Furanone-Producing Strains from a Collection of Gene Deletion Mutants of Saccharomyces cerevisiae.

2015 ◽  
Vol 110 (12) ◽  
pp. 840-848
Author(s):  
Kenji UEHARA ◽  
Jun WATANABE ◽  
Takeshi AKAO ◽  
Daisuke WATANABE ◽  
Yoshinobu MOGI ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Deletion ◽  
Deletion Mutants ◽  
High Level

scholarly journals Application of the comprehensive set of heterozygous yeast deletion mutants to elucidate the molecular basis of cellular chromium toxicity

2007 ◽  
Vol 8 (12) ◽  
pp. R268 ◽  
Author(s):  
Sara Holland ◽  
Emma Lodwig ◽  
Theodora Sideri ◽  
Tom Reader ◽  
Ian Clarke ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Deletion Mutants ◽  
Chromium Toxicity ◽  
Yeast Deletion

scholarly journals Screening of High-Level 4-Hydroxy-2 (or 5)-Ethyl-5 (or 2)-Methyl-3(2H)-Furanone-Producing Strains from a Collection of Gene Deletion Mutants of Saccharomyces cerevisiae

2014 ◽  
Vol 81 (1) ◽  
pp. 453-460 ◽  
Author(s):  
Kenji Uehara ◽  
Jun Watanabe ◽  
Takeshi Akao ◽  
Daisuke Watanabe ◽  
Yoshinobu Mogi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Deletion Mutant ◽  
Gene Deletion ◽  
Soy Sauce ◽  
Deletion Mutants ◽  
Flavor Compound ◽  
Content Type ◽  
Soybean Paste ◽  
High Level ◽  
Gene Deletion Mutant

ABSTRACT4-Hydroxy-2 (or 5)-ethyl-5 (or 2)-methyl-3(2H)-furanone (HEMF) is an important flavor compound that contributes to the sensory properties of many natural products, particularly soy sauce and soybean paste. The compound exhibits a caramel-like aroma and several important physiological activities, such as strong antioxidant activity. HEMF is produced by yeast species in soy sauce manufacturing; however, the enzymes involved in HEMF production remain unknown, hindering efforts to breed yeasts with high-level HEMF production. In this study, we identified high-level HEMF-producing mutants among aSaccharomyces cerevisiaegene deletion mutant collection. Fourteen deletion mutants were screened as high-level HEMF-producing mutants, and theADH1gene deletion mutant (adh1Δ) exhibited the maximum HEMF production capacity. Further investigations of theadh1Δ mutant implied that acetaldehyde accumulation contributes to HEMF production, agreeing with previous findings. Therefore, acetaldehyde might be a precursor for HEMF. TheADH1gene deletion mutant ofZygosaccharomyces rouxii, which is the dominant strain of yeast found during soy sauce fermentation, also produces HEMF effectively, suggesting that acetaldehyde accumulation might be a benchmark for breeding industrial yeasts with excellent HEMF production abilities.

scholarly journals Molecular Basis of Fructose Utilization by the Wine Yeast Saccharomyces cerevisiae: a Mutated HXT3 Allele Enhances Fructose Fermentation

2007 ◽  
Vol 73 (8) ◽  
pp. 2432-2439 ◽  
Author(s):  
Carole Guillaume ◽  
Pierre Delobel ◽  
Jean-Marie Sablayrolles ◽  
Bruno Blondin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Basis ◽  
Alcoholic Fermentation ◽  
Wine Yeast ◽  
Glycolytic Flux ◽  
Hexose Transporter ◽  
Wine Yeasts ◽  
Yeast Saccharomyces Cerevisiae ◽  
High Fructose ◽  
Fructose Fermentation

ABSTRACT Fructose utilization by wine yeasts is critically important for the maintenance of a high fermentation rate at the end of alcoholic fermentation. A Saccharomyces cerevisiae wine yeast able to ferment grape must sugars to dryness was found to have a high fructose utilization capacity. We investigated the molecular basis of this enhanced fructose utilization capacity by studying the properties of several hexose transporter (HXT) genes. We found that this wine yeast harbored a mutated HXT3 allele. A functional analysis of this mutated allele was performed by examining expression in an hxt1-7Δ strain. Expression of the mutated allele alone was found to be sufficient for producing an increase in fructose utilization during fermentation similar to that observed in the commercial wine yeast. This work provides the first demonstration that the pattern of fructose utilization during wine fermentation can be altered by expression of a mutated hexose transporter in a wine yeast. We also found that the glycolytic flux could be increased by overexpression of the mutant transporter gene, with no effect on fructose utilization. Our data demonstrate that the Hxt3 hexose transporter plays a key role in determining the glucose/fructose utilization ratio during fermentation.

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