scholarly journals Controllable gold-capped nanoporous anodic alumina chip for label-free, specific detection of bacterial cells

RSC Advances ◽  
2017 ◽  
Vol 7 (30) ◽  
pp. 18815-18820 ◽  
Author(s):  
Do-Kyun Kim ◽  
Dong Min Kim ◽  
Seung Min Yoo ◽  
Sang Yup Lee
Keyword(s):  
Anodic Alumina ◽  
Bacterial Cells ◽  
Label Free ◽  
Single Chip ◽  
Specific Detection ◽  
Nanoporous Anodic Alumina ◽  
Quantitative Manner

A gold-capped nanostructured PAA sensor that uses aptamers detected bacterial cells in a quantitative manner with high specificities on a single chip.

scholarly journals Real-Time Monitoring of Biotinylated Molecules Detection Dynamics in Nanoporous Anodic Alumina for Bio-Sensing

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 478 ◽  
Author(s):  
Laura Pol ◽  
Chris Eckstein ◽  
Laura Acosta ◽  
Elisabet Xifré-Pérez ◽  
Josep Ferré-Borrull ◽  
...  
Keyword(s):  
Surface Modification ◽  
Real Time ◽  
Anodic Alumina ◽  
Label Free ◽  
Real Time Monitoring ◽  
Helpful Tool ◽  
Nanoporous Anodic Alumina ◽  
Efficient Detection ◽  
Sensitivity And Selectivity ◽  
Molecule Model

The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have to be optimized. Real-time monitoring of all the reactions taking place during such modification steps can be a highly helpful tool for optimization. In this work, we propose nanoporous anodic alumina (NAA) functionalized with the streptavidin-biotin complex as a platform towards label-free biosensors. Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. The study compared the performance of different NAA pore sizes in order to achieve the highest response. Furthermore, the optimal streptavidin concentration that enabled the efficient detection of the biotinylated thrombin attachment was estimated. Finally, the ability of the NAA-RIfS system to quantify the concentration of biotinylated thrombin was evaluated. This study provides an optimized characterization method to monitor the chemical reactions that take place during the biotinylated molecules attachment within the NAA pores.

scholarly journals Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection

Sensors ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 4543 ◽  
Author(s):  
Laura Pol ◽  
Laura Karen Acosta ◽  
Josep Ferré-Borrull ◽  
Lluis F. Marsal
Keyword(s):  
Real Time ◽  
Anodic Alumina ◽  
Label Free ◽  
Chemical Modifications ◽  
Efficient Manner ◽  
Nanoporous Anodic Alumina ◽  
Chemically Modified ◽  
High Affinity ◽  
Interferometric Biosensor ◽  
Inner Pore

Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient manner. These chemical modifications must be well defined. In this work we propose nanoporous anodic alumina (NAA) chemically modified with streptavidin as a platform for aptamer immobilization. The immobilization of biotinylated thrombin binding aptamer (TBA) was monitored in real time by means of reflective interferometric spectroscopy (RIfS). The study has permitted to characterize in real time the path to immobilize TBA on the inner pore walls of NAA. Furthermore, this study provides an accurate label-free method to detect thrombin in real-time with high affinity and specificity.

Structural and Optical Nanoengineering of Nanoporous Anodic Alumina Rugate Filters for Real-Time and Label-Free Biosensing Applications

2014 ◽  
Vol 86 (3) ◽  
pp. 1837-1844 ◽  
Author(s):  
Tushar Kumeria ◽  
Mohammad Mahbubur Rahman ◽  
Abel Santos ◽  
Josep Ferré-Borrull ◽  
Lluís F. Marsal ◽  
...  
Keyword(s):  
Real Time ◽  
Anodic Alumina ◽  
Label Free ◽  
Nanoporous Anodic Alumina

Gold-Coated Ordered Nanoporous Anodic Alumina Bilayers for Future Label-Free Interferometric Biosensors

2013 ◽  
Vol 5 (16) ◽  
pp. 8093-8098 ◽  
Author(s):  
Gerard Macias ◽  
Laura P. Hernández-Eguía ◽  
Josep Ferré-Borrull ◽  
Josep Pallares ◽  
Lluís F. Marsal
Keyword(s):  
Anodic Alumina ◽  
Label Free ◽  
Nanoporous Anodic Alumina

Photoswitchable Membranes Based on Peptide‐Modified Nanoporous Anodic Alumina: Toward Smart Membranes for On‐Demand Molecular Transport

2015 ◽  
Vol 27 (19) ◽  
pp. 3019-3024 ◽  
Author(s):  
Tushar Kumeria ◽  
Jingxian Yu ◽  
Mohammed Alsawat ◽  
Mahaveer D. Kurkuri ◽  
Abel Santos ◽  
...  
Keyword(s):  
Molecular Transport ◽  
Anodic Alumina ◽  
Nanoporous Anodic Alumina ◽  
On Demand

Interfacial Super-Assembly of Nanofluidic Heterochannels from Layered Graphene and Alumina Oxide Arrays for Label-Free Histamine-Specific Detection

2021 ◽  
Vol 93 (5) ◽  
pp. 2982-2987
Author(s):  
Shan Zhou ◽  
Liping Zhang ◽  
Lei Xie ◽  
Jie Zeng ◽  
Beilei Qiu ◽  
...  
Keyword(s):  
Label Free ◽  
Specific Detection ◽  
Alumina Oxide

New 8-oxo-7,8-2´deoxyguanosine nanoporous anodic alumina aptasensor for colorectal cancer diagnosis in blood and urine

Nanoscale ◽  
2021 ◽  
Author(s):  
Luis Pla ◽  
Felix Sancenón ◽  
M.Carmen Martinez-Bisbal ◽  
Celia Banuls ◽  
Nuria Estañ ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colorectal Cancer ◽  
Cancer Diagnosis ◽  
Anodic Alumina ◽  
Human Diseases ◽  
Nanoporous Anodic Alumina

Many important human diseases, and specially cancer, have been related to the overproduction of 8-oxo-7,8-dihydro-2´-deoxyguanosine (8-oxo-dG). This molecule is a product of oxidative stress processes over nucleophilic bases in DNA....

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