scholarly journals Dissecting how modular polyketide synthase ketoreductases interact with acyl carrier protein-attached substrates

2017 ◽  
Vol 53 (83) ◽  
pp. 11457-11460 ◽  
Author(s):  
Luisa Moretto ◽  
Steven Vance ◽  
Brennan Heames ◽  
R. William Broadhurst
Keyword(s):  
Binding Site ◽  
Active Site ◽  
Polyketide Synthase ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Prosthetic Group ◽  
Correct Orientation ◽  
Interaction Studies ◽  
Modular Polyketide Synthase

Interaction studies show that KR domains possess a generic binding site for ACP domains and provide evidence that the 5′-phosphopantetheine prosthetic group plays a key role in delivering acyl substrates to the active site in the correct orientation.

scholarly journals Sticky swinging arm dynamics: studies of an acyl carrier protein domain from the mycolactone polyketide synthase

2016 ◽  
Vol 473 (8) ◽  
pp. 1097-1110 ◽  
Author(s):  
Steven Vance ◽  
Olga Tkachenko ◽  
Ben Thomas ◽  
Mona Bassuni ◽  
Hui Hong ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Acyl Carrier Protein ◽  
Protein Domain ◽  
Carrier Protein ◽  
Protein Surface ◽  
Prosthetic Group ◽  
Polyketide Biosynthesis ◽  
Covalently Linked

When covalently linked to an acyl carrier protein (ACP) and loaded with acyl substrate-mimics, some 4′-phosphopantetheine prosthetic group arms swing freely, whereas others stick to the protein surface, suggesting a possible mode of interaction with enzyme domains during polyketide biosynthesis.

scholarly journals Active site labeling of fatty acid and polyketide acyl-carrier protein transacylases

2019 ◽  
Vol 17 (19) ◽  
pp. 4720-4724 ◽  
Author(s):  
Tony D. Davis ◽  
Jennifer M. Michaud ◽  
Michael D. Burkart
Keyword(s):  
Fatty Acid ◽  
Fluorescent Probe ◽  
Active Site ◽  
Polyketide Synthase ◽  
Acyl Carrier Protein ◽  
Site Directed Mutagenesis ◽  
Probe Design ◽  
Directed Mutagenesis ◽  
Carrier Protein

Fluorescent probe design and site-directed mutagenesis unveil new activity-based chemical reporters for fatty acid and polyketide synthase acyl-carrier protein transacylases.

scholarly journals Solution structure and proposed domain-domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase

2007 ◽  
Vol 16 (10) ◽  
pp. 2093-2107 ◽  
Author(s):  
Viktor Y. Alekseyev ◽  
Corey W. Liu ◽  
David E. Cane ◽  
Joseph D. Puglisi ◽  
Chaitan Khosla
Keyword(s):  
Solution Structure ◽  
Polyketide Synthase ◽  
Acyl Carrier Protein ◽  
Protein Domain ◽  
Carrier Protein ◽  
Modular Polyketide Synthase

scholarly journals De Novo Design and Implementation of a Tandem Acyl Carrier Protein Domain in a Type I Modular Polyketide Synthase

2018 ◽  
Vol 13 (11) ◽  
pp. 3072-3077 ◽  
Author(s):  
Zilong Wang ◽  
Saket R. Bagde ◽  
Gerardo Zavala ◽  
Tsutomu Matsui ◽  
Xi Chen ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
De Novo ◽  
Acyl Carrier Protein ◽  
De Novo Design ◽  
Protein Domain ◽  
Carrier Protein ◽  
Type I ◽  
Design And Implementation ◽  
Modular Polyketide Synthase

scholarly journals Modular type I polyketide synthase acyl carrier protein domains share a common N-terminally extended fold

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Luisa Moretto ◽  
Rachel Heylen ◽  
Natalie Holroyd ◽  
Steven Vance ◽  
R. William Broadhurst
Keyword(s):  
Polyketide Synthase ◽  
Acyl Carrier Protein ◽  
Protein Domains ◽  
Carrier Protein ◽  
Type I
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