scholarly journals Generation of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides during the enzymatic hydrolysis of tropical banded cricket (Gryllodes sigillatus) proteins

2018 ◽  
Vol 9 (1) ◽  
pp. 407-416 ◽  
Author(s):  
Alice B. Nongonierma ◽  
Candice Lamoureux ◽  
Richard J. FitzGerald
Keyword(s):  
Enzymatic Hydrolysis ◽  
Dipeptidyl Peptidase ◽  
Protein Hydrolysates ◽  
Dipeptidyl Peptidase Iv ◽  
Inhibitory Peptides ◽  
Dpp Iv ◽  
Gryllodes Sigillatus ◽  
Hydrolysis Of

Cricket (Gryllodes sigillatus) protein hydrolysates inhibit dipeptidyl peptidase IV (DPP-IV) in vitro.

scholarly journals Quinoa (Chenopodium quinoa Willd.) protein hydrolysates with in vitro dipeptidyl peptidase IV (DPP-IV) inhibitory and antioxidant properties

2015 ◽  
Vol 65 ◽  
pp. 112-118 ◽  
Author(s):  
Alice B. Nongonierma ◽  
Solène Le Maux ◽  
Cécile Dubrulle ◽  
Chloé Barre ◽  
Richard J. FitzGerald
Keyword(s):  
Antioxidant Properties ◽  
Chenopodium Quinoa ◽  
Dipeptidyl Peptidase ◽  
Protein Hydrolysates ◽  
Dipeptidyl Peptidase Iv ◽  
Dpp Iv

scholarly journals A Novel Dipeptidyl Peptidase IV Inhibitory Tea Peptide Improves Pancreatic β-Cell Function and Reduces α-Cell Proliferation in Streptozotocin-Induced Diabetic Mice

2019 ◽  
Vol 20 (2) ◽  
pp. 322 ◽  
Author(s):  
Yating Lu ◽  
Peng Lu ◽  
Yu Wang ◽  
Xiaodong Fang ◽  
Jianming Wu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Type 2 Diabetes ◽  
Inhibitory Activity ◽  
Black Tea ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Inhibitory Peptides ◽  
Dpp Iv

Dipeptidyl peptidase IV (DPP-IV) inhibitors occupy a growing place in the drugs used for the management of type 2 diabetes. Recently, food components, including food-derived bioactive peptides, have been suggested as sources of DPP-IV inhibitors without side effects. Chinese black tea is a traditional health beverage, and it was used for finding DPP-IV inhibitory peptides in this study. The ultra-filtrated fractions isolated from the aqueous extracts of black tea revealed DPP-IV inhibitory activity in vitro. Four peptides under 1 kDa were identified by SDS-PAGE and LC-MS/MS (Liquid Chromatography-Mass Spectrometry-Mass Spectrometry) from the ultra-filtrate. The peptide II (sequence: AGFAGDDAPR), with a molecular mass of 976 Da, showed the greatest DPP-IV inhibitory activity (in vitro) among the four peptides. After administration of peptide II (400 mg/day) for 57 days to streptozotocin (STZ)-induced hyperglycemic mice, the concentration of glucagon-like peptide-1 (GLP-1) in the blood increased from 9.85 ± 1.96 pmol/L to 19.22 ± 6.79 pmol/L, and the insulin level was increased 4.3-fold compared to that in STZ control mice. Immunohistochemistry revealed the improved function of pancreatic beta-cells and suppressed proliferation of pancreatic alpha-cells. This study provides new insight into the use of black tea as a potential resource of food-derived DPP-IV inhibitory peptides for the management of type 2 diabetes.

scholarly journals Characterization of Novel Dipeptidyl Peptidase-IV Inhibitory Peptides from Soft-Shelled Turtle Yolk Hydrolysate Using Orthogonal Bioassay-Guided Fractionations Coupled with In Vitro and In Silico Study

2020 ◽  
Vol 13 (10) ◽  
pp. 308
Author(s):  
Nhung Thi Phuong Nong ◽  
Yu-Kuo Chen ◽  
Wen-Ling Shih ◽  
Jue-Liang Hsu
Keyword(s):  
Dipeptidyl Peptidase ◽  
Reversed Phase ◽  
Dipeptidyl Peptidase Iv ◽  
Inhibitory Peptides ◽  
Dpp Iv ◽  
Ic50 Value ◽  
In Silico Study ◽  
Liquid Chromatography Tandem Mass

Five novel peptides (LPLF, WLQL, LPSW, VPGLAL, and LVGLPL) bearing dipeptidyl peptidase IV (DPP-IV) inhibitory activities were identified from the gastrointestinal enzymatic hydrolysate of soft-shelled turtle yolk (SSTY) proteins. Peptides were isolated separately using reversed-phase (RP) chromatography in parallel with off-line strong cation exchange (SCX) chromatography followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to determine sequences. Among these peptides, LPSW showed the highest DPP-IV inhibitory activity with an IC50 value of 269.7 ± 15.91 µM. The results of the pre-incubation experiment and the kinetic study of these peptides indicated that WLQL is a true inhibitor and its inhibition toward DPP-IV is of an uncompetitive model, while LPLF, LPSW, and VPGLAL are real-substrates and competitive inhibitors against DPP-IV. The DPP-IV inhibitory peptides derived from SSTY hydrolysate in study are promising in the management of hyperglycemia in Type 2 diabetes.

Identification of novel dipeptidyl peptidase IV and α-glucosidase inhibitory peptides from Tenebrio molitor

2021 ◽  
Author(s):  
Fernando Rivero-Pino ◽  
Antonio Guadix ◽  
Emilia M. Guadix
Keyword(s):  
Enzymatic Hydrolysis ◽  
Tenebrio Molitor ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Antidiabetic Activity ◽  
Inhibitory Peptides ◽  
Dpp Iv ◽  
Glucosidase Inhibitors

Enzymatic hydrolysis improved the antidiabetic activity of Tenebrio molitor protein. Peptides below 2000 Da were the most bioactive. Some novel peptides were identified to be DPP-IV and α-glucosidase inhibitors.

scholarly journals Food protein hydrolysates as a source of dipeptidyl peptidase IV inhibitory peptides for the management of type 2 diabetes

2013 ◽  
Vol 73 (1) ◽  
pp. 34-46 ◽  
Author(s):  
Orla Power ◽  
A. B. Nongonierma ◽  
P. Jakeman ◽  
R. J. FitzGerald
Keyword(s):  
Type 2 Diabetes ◽  
Milk Proteins ◽  
Dipeptidyl Peptidase ◽  
Peptide Inhibitors ◽  
Protein Hydrolysates ◽  
Dipeptidyl Peptidase Iv ◽  
Food Protein ◽  
Inhibitory Peptides ◽  
Dpp Iv

The prevalence of type 2 diabetes mellitus (T2DM) is increasing and it is estimated that by 2030 approximately 366 million people will be diagnosed with this condition. The use of dipeptidyl peptidase IV (DPP-IV) inhibitors is an emerging strategy for the treatment of T2DM. DPP-IV is a ubiquitous aminodipeptidase that cleaves incretins such as glucagon like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), resulting in a loss in their insulinotropic activity. Synthetic DPP-IV drug inhibitors are being used to increase the half-life of the active GLP-1 and GIP. Dietary intervention is accepted as a key component in the prevention and management of T2DM. Therefore, identification of natural food protein-derived DPP-IV inhibitors is desirable. Peptides with DPP-IV inhibitory activity have been identified in a variety of food proteins. This review aims to provide an overview of food protein hydrolysates as a source of the DPP-IV inhibitory peptides with particular focus on milk proteins. In addition, the proposed modes of inhibition and structure–activity relationship of peptide inhibitors are discussed. Milk proteins and associated peptides also display insulinotropic activity and help regulate blood glucose in healthy and diabetic subjects. Therefore, milk protein derived peptide inhibitors may be a unique multifunctional peptide approach for the management of T2DM.

scholarly journals Identification of novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in camel milk protein hydrolysates

2018 ◽  
Vol 244 ◽  
pp. 340-348 ◽  
Author(s):  
Alice B. Nongonierma ◽  
Sara Paolella ◽  
Priti Mudgil ◽  
Sajid Maqsood ◽  
Richard J. FitzGerald
Keyword(s):  
Milk Protein ◽  
Dipeptidyl Peptidase ◽  
Protein Hydrolysates ◽  
Dipeptidyl Peptidase Iv ◽  
Camel Milk ◽  
Inhibitory Peptides ◽  
Dpp Iv

scholarly journals Release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from milk protein isolate (MPI) during enzymatic hydrolysis

2017 ◽  
Vol 94 ◽  
pp. 79-89 ◽  
Author(s):  
Alice B. Nongonierma ◽  
Caterina Mazzocchi ◽  
Sara Paolella ◽  
Richard J. FitzGerald
Keyword(s):  
Enzymatic Hydrolysis ◽  
Milk Protein ◽  
Dipeptidyl Peptidase ◽  
Protein Isolate ◽  
Dipeptidyl Peptidase Iv ◽  
Inhibitory Peptides ◽  
Dpp Iv

scholarly journals Generation of wheat gluten hydrolysates with dipeptidyl peptidase IV (DPP-IV) inhibitory properties

2017 ◽  
Vol 8 (6) ◽  
pp. 2249-2257 ◽  
Author(s):  
A. B. Nongonierma ◽  
M. Hennemann ◽  
S. Paolella ◽  
R. J. FitzGerald
Keyword(s):  
Wheat Gluten ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Inhibitory Peptides ◽  
Dpp Iv

Wheat gluten hydrolysates contain known/potential DPP-IV inhibitory peptides.

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