Fluorescent probes for sensitive and selective detection of pH changes in live cells in visible and near-infrared channels

Five fluorescent probes bearing coumarin moieties with spirolactam ring structures have been developed to detect pH changes in visible and near-infrared channels.

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Ratiometric Near-Infrared Fluorescent Probes Based On Through-Bond Energy Transfer and π-Conjugation Modulation between Tetraphenylethene and Hemicyanine Moieties for Sensitive Detection of pH Changes in Live Cells

Bioconjugate Chemistry ◽

10.1021/acs.bioconjchem.8b00111 ◽

2018 ◽

Vol 29 (4) ◽

pp. 1406-1418 ◽

Cited By ~ 33

Author(s):

Jianbo Wang ◽

Shuai Xia ◽

Jianheng Bi ◽

Mingxi Fang ◽

Wafa Mazi ◽

...

Keyword(s):

Energy Transfer ◽

Fluorescent Probes ◽

Bond Energy ◽

Near Infrared ◽

Sensitive Detection ◽

Live Cells ◽

Ph Changes

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Fluorescent probes based on π-conjugation modulation between hemicyanine and coumarin moieties for ratiometric detection of pH changes in live cells with visible and near-infrared channels

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2018.02.168 ◽

2018 ◽

Vol 265 ◽

pp. 699-708 ◽

Cited By ~ 20

Author(s):

Shuai Xia ◽

Jianbo Wang ◽

Jianheng Bi ◽

Xiao Wang ◽

Mingxi Fang ◽

...

Keyword(s):

Fluorescent Probes ◽

Near Infrared ◽

Live Cells ◽

Ph Changes ◽

Ratiometric Detection

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Near-infrared fluorescent probes based on TBET and FRET rhodamine acceptors with different pKa values for sensitive ratiometric visualization of pH changes in live cells

Journal of Materials Chemistry B ◽

10.1039/c8tb01524d ◽

2019 ◽

Vol 7 (2) ◽

pp. 198-209 ◽

Cited By ~ 28

Author(s):

Jianbo Wang ◽

Shuai Xia ◽

Jianheng Bi ◽

Yibin Zhang ◽

Mingxi Fang ◽

...

Keyword(s):

Fluorescent Probes ◽

Near Infrared ◽

Live Cells ◽

Pka Values ◽

Ph Changes ◽

Ph Variations

Three near-infrared ratiometric fluorescent probes with different pKa values were synthesized to achieve sensitive ratiometric visualization of pH variations in lysosomes.

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Turn-on visible and ratiometric near-infrared fluorescent probes for distinction endogenous esterases and chymotrypsins in live cells

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2019.127567 ◽

2020 ◽

Vol 306 ◽

pp. 127567 ◽

Cited By ~ 4

Author(s):

Jiemin Wang ◽

Zhidong Teng ◽

Ting Cao ◽

Jing Qian ◽

Lei Zheng ◽

...

Keyword(s):

Fluorescent Probes ◽

Near Infrared ◽

Live Cells ◽

Turn On

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Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes

Molecules ◽

10.3390/molecules24081592 ◽

2019 ◽

Vol 24 (8) ◽

pp. 1592 ◽

Cited By ~ 10

Author(s):

Fang ◽

Xia ◽

Bi ◽

Wigstrom ◽

Valenzano ◽

...

Keyword(s):

Detection Limit ◽

Oxygen Atom ◽

Membrane Permeability ◽

Fluorescent Probes ◽

Near Infrared ◽

Emission Spectra ◽

Live Cells ◽

Phenolic Oxygen ◽

Acetyl Group ◽

Low Cytotoxicity

Two near-infrared fluorescent probes (A and B) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both probes, an acetyl group is attached to the phenolic oxygen atom of the hemicyanine platform to decrease the probe fluorescence background. Probe A displays sensitive fluorescence responses and binds preferentially to Zn(II) ions over other metal ions such as Cd2+ ions with a low detection limit of 0.45 nM. In contrast, the emission spectra of probe B is not significantly affected if Zn(II) ions are added. Probe A possesses excellent membrane permeability and low cytotoxicity, allowing for sensitive imaging of both exogenously supplemented Zn(II) ions in live cells, and endogenously releases Zn(II) ions in cells after treatment of 2,2-dithiodipyridine.

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