Turn-on visible and ratiometric near-infrared fluorescent probes for distinction endogenous esterases and chymotrypsins in live cells

2020 ◽  
Vol 306 ◽  
pp. 127567 ◽  
Author(s):  
Jiemin Wang ◽  
Zhidong Teng ◽  
Ting Cao ◽  
Jing Qian ◽  
Lei Zheng ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Live Cells ◽  
Turn On

scholarly journals Design of Turn‐On Near‐Infrared Fluorescent Probes for Highly Sensitive and Selective Monitoring of Biopolymers

2020 ◽  
Vol 59 (22) ◽  
pp. 8440-8444 ◽  
Author(s):  
Gerard T. Ducharme ◽  
Zane LaCasse ◽  
Tanya Sheth ◽  
Irina V. Nesterova ◽  
Evgueni E. Nesterov
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Turn On ◽  
Highly Sensitive ◽  
Selective Monitoring

scholarly journals Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1592 ◽  
Author(s):  
Fang ◽  
Xia ◽  
Bi ◽  
Wigstrom ◽  
Valenzano ◽  
...  
Keyword(s):  
Detection Limit ◽  
Oxygen Atom ◽  
Membrane Permeability ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Emission Spectra ◽  
Live Cells ◽  
Phenolic Oxygen ◽  
Acetyl Group ◽  
Low Cytotoxicity

Two near-infrared fluorescent probes (A and B) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both probes, an acetyl group is attached to the phenolic oxygen atom of the hemicyanine platform to decrease the probe fluorescence background. Probe A displays sensitive fluorescence responses and binds preferentially to Zn(II) ions over other metal ions such as Cd2+ ions with a low detection limit of 0.45 nM. In contrast, the emission spectra of probe B is not significantly affected if Zn(II) ions are added. Probe A possesses excellent membrane permeability and low cytotoxicity, allowing for sensitive imaging of both exogenously supplemented Zn(II) ions in live cells, and endogenously releases Zn(II) ions in cells after treatment of 2,2-dithiodipyridine.

scholarly journals Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging

RSC Advances ◽  
2019 ◽  
Vol 9 (71) ◽  
pp. 41431-41437 ◽  
Author(s):  
Shaolong Qi ◽  
Lubao Zhu ◽  
Xinyu Wang ◽  
Jianshi Du ◽  
Qingbiao Yang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Biological Samples ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Signal To Noise Ratio ◽  
High Signal ◽  
Biological Detection ◽  
Signal To Noise ◽  
Turn On

Near-infrared (NIR) fluorescent probes are widely employed in biological detection because of their lower damage to biological samples, low background interference, and high signal-to-noise ratio.

Gold Nanocluster-Based Fluorescent Probes for Near-Infrared and Turn-On Sensing of Glutathione in Living Cells

Langmuir ◽  
2012 ◽  
Vol 28 (8) ◽  
pp. 3945-3951 ◽  
Author(s):  
Dahui Tian ◽  
Zhangsheng Qian ◽  
Yunsheng Xia ◽  
Changqing Zhu
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Living Cells ◽  
Gold Nanocluster ◽  
Turn On

scholarly journals Cancer-Specific hNQO1-Responsive Biocompatible Naphthalimides Providing a Rapid Fluorescent Turn-On with an Enhanced Enzyme Affinity

Sensors ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 53 ◽  
Author(s):  
Sun Young Park ◽  
Eugeine Jung ◽  
Jong Seung Kim ◽  
Sung-Gil Chi ◽  
Min Hee Lee
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probes ◽  
Therapeutic Agent ◽  
Cell Imaging ◽  
Physiological Condition ◽  
Live Cells ◽  
Strong Fluorescence ◽  
Turn On ◽  
Efficient Detection ◽  
Agent Development

Human NAD(P)H:quinone oxidoreductase 1 (hNQO1) is overexpressed in cancer cells and associated with the drug resistance factor of cancer. The objective of this work is the development of fluorescent probes for the efficient detection of hNQO1 activity in cancer cells, which can be employed for the cancer diagnosis and therapeutic agent development. Herein, we report naphthalimide-based fluorescent probes 1 and 2 that can detect hNQO1. For hNQO1 activity, the probes showed a significant fluorescence increase at 540 nm. In addition, probe 1, the naphthalimide containing a triphenylphosphonium salt, showed an enhanced enzyme efficiency and rapid detection under a physiological condition. The detection ability of probe 1 was superior to that of other previously reported probes. Moreover, probe 1 was less cytotoxic during the cancer cell imaging and readily provided a strong fluorescence in hNQO1-overexpressed cancer cells (A549). We proposed that probe 1 can be used to detect hNQO1 expression in live cells and it will be applied to develop the diagnosis and customized treatment of hNQO1-related disease.

scholarly journals Fluorescent probes for sensitive and selective detection of pH changes in live cells in visible and near-infrared channels

2017 ◽  
Vol 5 (48) ◽  
pp. 9579-9590 ◽  
Author(s):  
Mingxi Fang ◽  
Rashmi Adhikari ◽  
Jianheng Bi ◽  
Wafa Mazi ◽  
Nethaniah Dorh ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Live Cells ◽  
Selective Detection ◽  
Ph Changes ◽  
Ring Structures

Five fluorescent probes bearing coumarin moieties with spirolactam ring structures have been developed to detect pH changes in visible and near-infrared channels.

Reaction-based turn-on fluorescent probes with magnetic responses for Fe2+ detection in live cells

2015 ◽  
Vol 44 (19) ◽  
pp. 8942-8949 ◽  
Author(s):  
Siddhartha Maiti ◽  
Ziya Aydin ◽  
Yi Zhang ◽  
Maolin Guo
Keyword(s):  
Fluorescent Probes ◽  
Fluorescent Sensor ◽  
Live Cells ◽  
Turn On ◽  
Selective Reaction

A highly selective reaction-based “turn-on” fluorescent sensor is capable of detecting Fe2+ in mitochondria with distinct EPR responses.

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