Selection of DNA aptamers and establishment of an effective aptasensor for highly sensitive detection of cefquinome residues in milk

The Analyst ◽  
2018 ◽  
Vol 143 (13) ◽  
pp. 3202-3208 ◽  
Author(s):  
Lihui Wang ◽  
Chuchu Wang ◽  
Hao Li
Keyword(s):  
Respiratory Infection ◽  
Bovine Mastitis ◽  
Generation Cephalosporin ◽  
Sensitive Detection ◽  
Fourth Generation ◽  
Dna Aptamers ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Selection Of

Cefquinome (CFQ), which is a fourth-generation cephalosporin approved for veterinary use only, has been widely used for treating porcine or bovine respiratory infection, bovine mastitis and other diseases.

Enzyme linking to DNA aptamers via a zinc finger as a bridge

2015 ◽  
Vol 51 (57) ◽  
pp. 11467-11469 ◽  
Author(s):  
Koichi Abe ◽  
Yoshiyuki Murakami ◽  
Atsuro Tatsumi ◽  
Koichi Sumida ◽  
Aki Kezuka ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Detection System ◽  
Sensitive Detection ◽  
Dna Aptamers ◽  
Recognition Sequence ◽  
System P ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Aptamers are labeled by luciferase via a zinc finger (ZF) by introduction into a ZF recognition sequence to the aptamer. It enables us to construct a highly sensitive detection system.

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

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