Fabrication of 3D continuous-flow reverse-transcription polymerase chain reaction microdevice integrated with on-chip fluorescence detection for semi-quantitative assessment of gene expression

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5692-5701
Author(s):  
Quang Nghia Pham ◽  
Kieu The Loan Trinh ◽  
Nguyen Khoi Song Tran ◽  
Tae-Sik Park ◽  
Nae Yoon Lee
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Continuous Flow ◽  
Quantitative Assessment ◽  
Chain Reaction ◽  
Rna Amplification ◽  
Portable Pump ◽  
Polymerase Chain ◽  
On Chip ◽  
Single Heater

A 3D microdevice equipped with a portable pump and a single heater was fabricated integrating RNA amplification and detection functionalities.

Quantitative assessment of gene expression in highly purified hematopoietic cells using real-time reverse transcriptase polymerase chain reaction

2002 ◽  
Vol 30 (5) ◽  
pp. 481-487 ◽  
Author(s):  
Marc H.G.P. Raaijmakers ◽  
Liesbeth van Emst ◽  
Theo de Witte ◽  
Ewald Mensink ◽  
Reinier A.P. Raymakers
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Quantitative Assessment ◽  
Hematopoietic Cells ◽  
Chain Reaction ◽  
Polymerase Chain

Microfabricated Device for DNA and RNA Amplification by Continuous-Flow Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction with Cycle Number Selection

2003 ◽  
Vol 75 (2) ◽  
pp. 288-295 ◽  
Author(s):  
Pierre J. Obeid ◽  
Theodore K. Christopoulos ◽  
H. John Crabtree ◽  
Christopher J. Backhouse
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Continuous Flow ◽  
Chain Reaction ◽  
Rna Amplification ◽  
Cycle Number ◽  
Dna And Rna ◽  
Polymerase Chain

Bent polydimethylsiloxane–polycarbonate hybrid microdevice for on-chip flow-through polymerase chain reaction employing a single heater

2014 ◽  
Vol 181 (13-14) ◽  
pp. 1697-1705 ◽  
Author(s):  
Kieu The Loan Trinh ◽  
Wenming Wu ◽  
Nae Yoon Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Chip Flow ◽  
Flow Through ◽  
Polymerase Chain ◽  
On Chip ◽  
Single Heater

Bubble-free on-chip continuous-flow polymerase chain reaction: concept and application

The Analyst ◽  
2011 ◽  
Vol 136 (11) ◽  
pp. 2287 ◽  
Author(s):  
Wenming Wu ◽  
Kyung-Tae Kang ◽  
Nae Yoon Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Continuous Flow ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
On Chip

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Marianne Jorgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Anti-Inflammatory Effects of Abeliophyllum distichum Nakai (Cultivar Okhwang 1) Callus through Inhibition of PI3K/Akt, NF-κB, and MAPK Signaling Pathways in Lipopolysaccharide-Induced Macrophages

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1071
Author(s):  
Tae-Won Jang ◽  
Jae-Ho Park
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Signaling Pathways ◽  
Chain Reaction ◽  
Inos Gene ◽  
Polymerase Chain ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Abeliophyllum Distichum ◽  
Inos Gene Expression

One of the Korean endemic plants, Abeliophyllum distichum Nakai (Oleaceae), contains acteoside, which is a glycoside exhibiting neuroprotective, anti-inflammation effects and antibacterial capacities. We conducted an investigation on the effects of the callus of A. distichum (cultivar Okhwang 1, CAO) on pro-inflammatory mediators released following nuclear factor-кB (NF-кB), phosphatidylinositol 3-kinase/Akt (PI3K-Akt) and mitogen-activated protein kinase (MAPK) signal activation in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Immunoblotting was employed to find out the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide (iNOS), and activation of MAPK molecules, NF-κB and Akt. Cytokines, COX-2, and iNOS gene expression were assessed using polymerase chain reaction techniques. Cytokines, COX-2, and iNOS gene expression were assessed using polymerase chain reaction techniques. High-performance liquid chromatography revealed that CAO was rich in acteoside and isoacteoside. As a result, CAO inhibited the generation of NO, cytokines, COX-2, and iNOS expression. Further, translocation to the nuclear of NF-κB p65 and degradation of the inhibitor of NF-кB (IкB) were alleviated by suppressing phosphorylation. Additionally, CAO significantly impacted MAPK pathway activation by potentially reducing phosphorylation of MAPKs. These results indicate that the anti-inflammatory effect of CAO is mediated via the inhibition of MAPK, PI3K/Akt, and NF-κB signaling pathways, probably via glycosides, phenolics, and flavonoids bioactivity derived from plants. CAO can serve as a potential anti-inflammatory agent, which alleviates inflammation factors and act through specific cell signaling pathways.

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