Reaction-based fluorescent probes for rapid detection of hydrogen sulfide in vivo

2018 ◽  
Vol 10 (33) ◽  
pp. 4079-4084 ◽  
Author(s):  
Xilang Jin ◽  
Xianglong Wu ◽  
Pu Xie ◽  
Sha Liu ◽  
Jie Wu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Real Time ◽  
Fluorescent Probes ◽  
Rapid Detection ◽  
High Sensitivity ◽  
Fast Response ◽  
Real Time Detection

The probe exhibited high sensitivity, selectivity, and fast response for real-time detection of H2S in vivo.

scholarly journals Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Helmut Bischof ◽  
Markus Rehberg ◽  
Sarah Stryeck ◽  
Katharina Artinger ◽  
Emrah Eroglu ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probes ◽  
Real Time Detection

scholarly journals A novel method for visualizing and tracking endogenous mRNA in a specific cell population in pathological neovascularization

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Md Imam Uddin ◽  
Tyler C. Kilburn ◽  
Sara Z. Jamal ◽  
Craig L. Duvall ◽  
John S. Penn
Keyword(s):  
Real Time ◽  
Disease Burden ◽  
Ex Vivo ◽  
Expression Patterns ◽  
Fluorescence Emission ◽  
High Sensitivity ◽  
Living Systems ◽  
Specific Cell ◽  
Potential Marker

AbstractDiabetic retinopathy, retinopathy of prematurity and retinal vein occlusion are potentially blinding conditions largely due to their respective neovascular components. The development of real-time in vivo molecular imaging methods, to assess levels of retinal neovascularization (NV), would greatly benefit patients afflicted with these conditions. mRNA hybridization techniques offer a potential method to image retinal NV. The success of these techniques hinges on the selection of a target mRNA whose tissue levels and spatial expression patterns correlate closely with disease burden. Using a model of oxygen-induced retinopathy (OIR), we previously observed dramatic increases in retinal endoglin that localized to neovascular structures (NV), directly correlating with levels of neovascular pathology. Based on these findings, we have investigated Endoglin mRNA as a potential marker for imaging retinal NV in OIR mice. Also of critical importance, is the application of innovative technologies capable of detecting mRNAs in living systems with high sensitivity and specificity. To detect and visualize endoglin mRNA in OIR mice, we have designed and synthesized a novel imaging probe composed of short-hairpin anti-sense (AS) endoglin RNA coupled to a fluorophore and black hole quencher (AS-Eng shRNA). This assembly allows highly sensitive fluorescence emission upon hybridization of the AS-Eng shRNA to cellular endoglin mRNA. The AS-Eng shRNA is further conjugated to a diacyl-lipid (AS-Eng shRNA–lipid referred to as probe). The lipid moiety binds to serum albumin facilitating enhanced systemic circulation of the probe. OIR mice received intraperitoneal injections of AS-Eng shRNA–lipid. Ex vivo imaging of their retinas revealed specific endoglin mRNA dependent fluorescence superimposed on neovascular structures. Room air mice receiving AS-Eng shRNA–lipid and OIR mice receiving a non-sense control probe showed little fluorescence activity. In addition, we found that cells in neovascular lesions labelled with endoglin mRNA dependent fluorescence, co-labelled with the macrophage/microglia-associated marker IBA1. Others have shown that cells expressing macrophage/microglia markers associate with retinal neovascular structures in proportion to disease burden. Hence we propose that our probe may be used to image and to estimate the levels of retinal neovascular disease in real-time in living systems.

scholarly journals Biological nanoscale fluorescent probes: From structure and performance to bioimaging

2020 ◽  
Vol 39 (1) ◽  
pp. 209-221
Author(s):  
Jiafeng Wan ◽  
Xiaoyuan Zhang ◽  
Kai Zhang ◽  
Zhiqiang Su
Keyword(s):  
Fluorescent Probes ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Sensitivity ◽  
Biological Imaging ◽  
Fluorescent Materials ◽  
And Performance

Abstract In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc., are widely used in biological imaging due to their high sensitivity, short response time, and excellent accuracy. Nanometer fluorescent probes can not only perform in vitro imaging of organisms but also achieve in vivo imaging. This provides medical staff with great convenience in cancer treatment. Combined with contemporary medical methods, faster and more effective treatment of cancer is achievable. This article explains the response mechanism of three-nanometer fluorescent probes: the principle of induced electron transfer (PET), the principle of fluorescence resonance energy transfer (FRET), and the principle of intramolecular charge transfer (ICT), showing the semiconductor QDs, precious MNCs, and CDs. The excellent performance of the three kinds of nano fluorescent materials in biological imaging is highlighted, and the application of these three kinds of nano fluorescent probes in targeted biological imaging is also introduced. Nanometer fluorescent materials will show their significance in the field of biomedicine.

A reversible near-infrared fluorescence probe for the monitoring of HSO3–/H2O2 regulated cycles in vivo

2021 ◽  
Author(s):  
Qiuying Song ◽  
Bo Zhou ◽  
Dongyu Zhang ◽  
Haijun Chi ◽  
Hongmin Jia ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Fluorescence Probe ◽  
Redox Homeostasis ◽  
Research Field ◽  
Fluorescence Probes ◽  
Detection Capability ◽  
Near Infrared Fluorescence ◽  
Real Time Detection

The development of well-designed fluorescence probes for the monitoring redox homeostasis in biosystems has become a desired research field owing to their noninvasive and real-time detection capability in vivo. In...

Ratiometric fluorescent probes with a self-immolative spacer for real-time detection of β-galactosidase and imaging in living cells

2018 ◽  
Vol 1033 ◽  
pp. 193-198 ◽  
Author(s):  
Xiangzhu Chen ◽  
Xiaodong Ma ◽  
Yuanyuan Zhang ◽  
Gui Gao ◽  
Jingjing Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Real Time Detection

scholarly journals A fast response fluorescence probe specific for hypochlorous acid detection and its applications in bioimaging

2018 ◽  
Vol 16 (12) ◽  
pp. 2074-2082 ◽  
Author(s):  
Hongmin Jia ◽  
Shuhe Xia ◽  
Huan Feng ◽  
Qingtao Meng ◽  
Chengchen Duan ◽  
...  
Keyword(s):  
Hypochlorous Acid ◽  
Fluorescence Probe ◽  
High Sensitivity ◽  
Fast Response ◽  
Rapid Response

The features ofDNPH-NA, including its high sensitivity, selectivity, and reliability at physiological pH, together with a rapid response, enable its successful application in the detection of endogenous HOClin vitroandin vivo.

scholarly journals Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species

2016 ◽  
Vol 9 ◽  
pp. MBI.S38517 ◽  
Author(s):  
Jing Zhang ◽  
Guo-Chiuan Hung ◽  
Kenjiro Nagamine ◽  
Bingjie Li ◽  
Shien Tsai ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Candida Species ◽  
Rapid Detection ◽  
Corneal Transplantation ◽  
High Sensitivity ◽  
Turnaround Time ◽  
Pcr Assays ◽  
Primer Sets ◽  
Pcr Primer

Culture-based identification methods have been the gold standard for the diagnosis of fungal infection. Currently, molecular technologies such as real-time PCR assays with short turnaround time can provide desirable alternatives for the rapid detection of Candida microbes. However, most of the published PCR primer sets are not Candida specific and likely to amplify DNA from common environmental contaminants, such as Aspergillus microbes. In this study, we designed pan- Candida primer sets based on the ribosomal DNA-coding regions conserved within Candida but distinct from those of Aspergillus and Penicillium. We demonstrate that the final two selected pan- Candida primer sets would not amplify Aspergillus DNA and could be used to differentiate eight medically important Candida pathogens in real-time PCR assays based on their melting profiles, with a sensitivity of detection as low as 10 fg of Candida genomic DNA. Moreover, we further evaluated and selected species-specific primer sets covering Candida albicans, Candida glabrata, Candida tropicalis, and Candida dubliniensis and show that they had high sensitivity and specificity. These real-time PCR primer sets could potentially be assembled into a single PCR array for the rapid detection of Candida species in various clinical settings, such as corneal transplantation.

A remarkable ratiometric fluorescent chemodosimeter for very rapid detection of hydrogen sulfide in the vapour phase and living cells

2015 ◽  
Vol 39 (11) ◽  
pp. 8940-8947 ◽  
Author(s):  
Sima Paul ◽  
Shyamaprosad Goswami ◽  
Chitrangada Das Mukhopadhyay
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Rapid Detection ◽  
Stokes Shift ◽  
Vapour Phase ◽  
Fast Response ◽  
Living Cells ◽  
Fluorescent Chemodosimeter ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe having a fast response and a large Stokes shift detects SH− both in solid and vapour phases and this probe is used for fluorescence imaging of SH− in living cells.

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