scholarly journals Chloroplast-containing coacervate micro-droplets as a step towards photosynthetically active membrane-free protocells

2018 ◽  
Vol 54 (29) ◽  
pp. 3594-3597 ◽  
Author(s):  
B. V. V. S. Pavan Kumar ◽  
James Fothergill ◽  
Joshua Bretherton ◽  
Liangfei Tian ◽  
Avinash J. Patil ◽  
...  

Encapsulation of structurally and functionally intact chloroplasts within coacervate micro-droplets is used to prepare photosynthetically active membrane-free protocells.


2021 ◽  
Vol 295 ◽  
pp. 116950
Author(s):  
Andrew J. Fix ◽  
James E. Braun ◽  
David M. Warsinger


2020 ◽  
Vol 66 (4) ◽  
pp. 760-770
Author(s):  
Liang Fan ◽  
Liang-liang Lv ◽  
Fu-jun Peng ◽  
Guo-ping Cai


2014 ◽  
Vol 276 ◽  
pp. 415-421 ◽  
Author(s):  
Qing Wang ◽  
Yi Li ◽  
Chao Wang ◽  
Yue Wu ◽  
Peifang Wang


1984 ◽  
Vol 39 (6) ◽  
pp. 627-633 ◽  
Author(s):  
Ji-yu Ye ◽  
U. Heber

The effect of aureomycin on photosynthesis was investigated. This antibiotic which has been reported to stimulate photosynthesis at very low concentrations is an effective inhibitor at higher concentrations. In mesophyll protoplasts and isolated chloroplasts from spinach, 50% inhibition of CO2 reduction required about 20 μᴍ aureomycin. The reduction of 3-phosphoglycerate and of oxaloacetate by intact chloroplasts was also inhibited, but not that of nitrite and methylviologen which was actually stimulated. NADP reduction by broken chloroplasts and methylviologen- dependent photophosphorylation were also sensitive to aureomycin. The electrochromic shift at 518 nm which indicates formation of a light-dependent membrane potential was suppressed in the presence of 200 μᴍ aureomycin and the transthylakoid proton gradient was decreased. The data confirm reports that aureomycin has uncoupling properties, and they indicate that it also acts as an inhibitor of ferredoxin/NADP reductase.



1980 ◽  
Vol 86 (1) ◽  
pp. 49-61
Author(s):  
G. F. GWILLIAM ◽  
M. BURROWS

1. The electrical properties of the membrane of an identified locust motor neurone, the fast extensor tibiae in the metathoracic ganglion, have been investigated to determine: the distribution of excitable and inexcitable membrane; the impulse initiation zone; and the conduction velocity of the spike in the ganglion and in the axon. 2. The waveform of extracellularly recorded spikes indicates that the transition from inactive to active membrane occurs along the region of the neurite which bears many arborizations within the neuropile. 3. Measurements of the delay between orthodromically or antidromically evoked spikes, recorded at the soma and other points along the neurite, place the impulse initiating zone close to the transition between active and inactive membrane. 4. Within the ganglion, the spike is conducted at different velocities over different parts of the neurite. The average velocity within the ganglion is, however, only about a seventh of that in the axon (0.54 m.s−1 against 4.1 m.s−1).



2001 ◽  
Vol 86 (6) ◽  
pp. 2667-2677 ◽  
Author(s):  
Gloria J. Klapstein ◽  
Robin S. Fisher ◽  
Hadi Zanjani ◽  
Carlos Cepeda ◽  
Eve S. Jokel ◽  
...  

We examined passive and active membrane properties and synaptic responses of medium-sized spiny striatal neurons in brain slices from presymptomatic (∼40 days of age) and symptomatic (∼90 days of age) R6/2 transgenics, a mouse model of Huntington's disease (HD) and their age-matched wild-type (WT) controls. This transgenic expresses exon 1 of the human HD gene with ∼150 CAG repeats and displays a progressive behavioral phenotype associated with numerous neuronal alterations. Intracellular recordings were obtained using standard techniques from R6/2 and age-matched WT mice. Few electrophysiological changes occurred in striatal neurons from presymptomatic R6/2 mice. The changes in this age group were increased neuronal input resistance and lower stimulus intensity to evoke action potentials (rheobase). Symptomatic R6/2 mice exhibited numerous electrophysiological alterations, including depolarized resting membrane potentials, increased input resistances, decreased membrane time constants, and alterations in action potentials. Increased stimulus intensities were required to evoke excitatory postsynaptic potentials (EPSPs) in neurons from symptomatic R6/2 transgenics. These EPSPs had slower rise times and did not decay back to baseline by 45 ms, suggesting a more prominent component mediated by activation of N-methyl-d-aspartate receptors. Neurons from both pre- and symptomatic R6/2 mice exhibited reduced paired-pulse facilitation. Data from biocytin-filled or Golgi-impregnated neurons demonstrated decreased dendritic spine densities, smaller diameters of dendritic shafts, and smaller dendritic fields in symptomatic R6/2 mice. Taken together, these findings indicate that passive and active membrane and synaptic properties of medium-sized spiny neurons are altered in the R6/2 transgenic. These physiological and morphological alterations will affect communication in the basal ganglia circuitry. Furthermore, they suggest areas to target for pharmacotherapies to alleviate and reduce the symptoms of HD.



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