Visualization of mitochondrial DNA in living cells with super-resolution microscopy using thiophene-based terpyridine Zn(ii) complexes

2018 ◽  
Vol 54 (80) ◽  
pp. 11288-11291 ◽  
Author(s):  
Yu Shen ◽  
Tao Shao ◽  
Bin Fang ◽  
Wei Du ◽  
Mingzhu Zhang ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Super Resolution ◽  
Living Cells ◽  
Intercalative Binding ◽  
Super Resolution Microscopy

The thiophene-based terpyridine Zn(ii) complex LC targeted mitochondria by intercalative binding with mtDNA. STED super-resolution micrographs visualized mitochondrial cristae/inner matrix mtDNA.

scholarly journals LIVE-PAINT allows super-resolution microscopy inside living cells using reversible peptide-protein interactions

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Curran Oi ◽  
Zoe Gidden ◽  
Louise Holyoake ◽  
Owen Kantelberg ◽  
Simon Mochrie ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Super Resolution ◽  
Living Cells ◽  
Super Resolution Microscopy

scholarly journals Intracellular Delivery of Membrane Impermeable Photostable Fluorescent Probes into Living Cells for Super-Resolution Microscopy

2017 ◽  
Vol 112 (3) ◽  
pp. 21a
Author(s):  
Yuji Ishitsuka ◽  
Kai Wen Teng ◽  
Pin Ren ◽  
Yeoan Youn ◽  
Xiang Deng ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Super Resolution ◽  
Intracellular Delivery ◽  
Living Cells ◽  
Super Resolution Microscopy

scholarly journals Nano-scale size holes in ER sheets provide an alternative to tubules for highly-curved membranes

2017 ◽  
Author(s):  
Lena K. Schroeder ◽  
Andrew E. S. Barentine ◽  
Sarah Schweighofer ◽  
David Baddeley ◽  
Joerg Bewersdorf ◽  
...  
Keyword(s):  
Analytical Modeling ◽  
High Spatial Resolution ◽  
Super Resolution ◽  
Living Cells ◽  
Stimulated Emission ◽  
Fast Time ◽  
Nano Scale ◽  
Scale Size ◽  
Curved Membranes ◽  
Super Resolution Microscopy

AbstractThe endoplasmic reticulum (ER) is composed of interconnected membrane sheets and tubules. Super-resolution microscopy recently revealed densely packed, rapidly moving ER tubules, highlighting the importance of revisiting classical views of ER structure with high spatial resolution in living cells. Using live-cell Stimulated Emission Depletion (STED) microscopy, we show highly dynamic, subdiffraction-sized holes in ER sheets. Holes coexist with uniform sheet regions and are distinct from tubular ER structures. The curvature-stabilizing reticulon protein Rtn4 localizes to these holes and the ER luminal tether Climp63 controls their diameter and mobility. Analytical modeling demonstrates that holes in ER sheets can serve as reservoirs for curvature-stabilizing proteins to support ER tubule extension and retraction, thus providing an explanation for how the ER locally alters its morphology on fast time-scales.One Sentence SummaryDynamic nano-scale sized holes are prominent features of ER sheets that serve as reservoirs for curvature-stabilizing proteins to support ER tubule extension and retraction.

scholarly journals Single-Molecule Super-Resolution Microscopy Reveals Heteromeric Complexes of MET and EGFR upon Ligand Activation

2020 ◽  
Vol 21 (8) ◽  
pp. 2803 ◽  
Author(s):  
Marie-Lena I.E. Harwardt ◽  
Mark S. Schröder ◽  
Yunqing Li ◽  
Sebastian Malkusch ◽  
Petra Freund ◽  
...  
Keyword(s):  
Single Molecule ◽  
Tyrosine Kinases ◽  
Growth Factor Receptor ◽  
Super Resolution ◽  
Cell Types ◽  
Surface Expression ◽  
Living Cells ◽  
Ligand Activation ◽  
Receptor Clusters ◽  
Super Resolution Microscopy

Receptor tyrosine kinases (RTKs) orchestrate cell motility and differentiation. Deregulated RTKs may promote cancer and are prime targets for specific inhibitors. Increasing evidence indicates that resistance to inhibitor treatment involves receptor cross-interactions circumventing inhibition of one RTK by activating alternative signaling pathways. Here, we used single-molecule super-resolution microscopy to simultaneously visualize single MET and epidermal growth factor receptor (EGFR) clusters in two cancer cell lines, HeLa and BT-20, in fixed and living cells. We found heteromeric receptor clusters of EGFR and MET in both cell types, promoted by ligand activation. Single-protein tracking experiments in living cells revealed that both MET and EGFR respond to their cognate as well as non-cognate ligands by slower diffusion. In summary, for the first time, we present static as well as dynamic evidence of the presence of heteromeric clusters of MET and EGFR on the cell membrane that correlates with the relative surface expression levels of the two receptors.

scholarly journals Dissecting the actin cortex density and membrane-cortex distance in living cells by super-resolution microscopy

2017 ◽  
Vol 50 (6) ◽  
pp. 064002 ◽  
Author(s):  
M P Clausen ◽  
H Colin-York ◽  
F Schneider ◽  
C Eggeling ◽  
M Fritzsche
Keyword(s):  
Super Resolution ◽  
Living Cells ◽  
Actin Cortex ◽  
Super Resolution Microscopy

scholarly journals Super‐Resolution Microscopy Using a Bioorthogonal‐Based Cholesterol Probe Provides Unprecedented Capabilities for Imaging Nanoscale Lipid Heterogeneity in Living Cells

Small Methods ◽  
2021 ◽  
pp. 2100430
Author(s):  
Maier Lorizate ◽  
Oihana Terrones ◽  
Jon Ander Nieto‐Garai ◽  
Iratxe Rojo‐Bartolomé ◽  
Dalila Ciceri ◽  
...  
Keyword(s):  
Super Resolution ◽  
Living Cells ◽  
Super Resolution Microscopy

scholarly journals Video-Rate Super Resolution Microscopy in Living Cells

2015 ◽  
Vol 108 (2) ◽  
pp. 475a
Author(s):  
Fang Huang ◽  
Caroline E. Laplante ◽  
Yu Lin ◽  
Thomas D. Pollard ◽  
Joerg Bewersdorf
Keyword(s):  
Super Resolution ◽  
Living Cells ◽  
Super Resolution Microscopy

scholarly journals Structured illumination microscopy and its new developments

2016 ◽  
Vol 09 (03) ◽  
pp. 1630010 ◽  
Author(s):  
Jianling Chen ◽  
Caimin Qiu ◽  
Minghai You ◽  
Xiaogang Chen ◽  
Hongqin Yang ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Spatial Resolution ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Living Cells ◽  
The Other ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Recent Developments ◽  
Super Resolution Microscopy

Optical microscopy allows us to observe the biological structures and processes within living cells. However, the spatial resolution of the optical microscopy is limited to about half of the wavelength by the light diffraction. Structured illumination microscopy (SIM), a type of new emerging super-resolution microscopy, doubles the spatial resolution by illuminating the specimen with a patterned light, and the sample and light source requirements of SIM are not as strict as the other super-resolution microscopy. In addition, SIM is easier to combine with the other imaging techniques to improve their imaging resolution, leading to the developments of diverse types of SIM. SIM has great potential to meet the various requirements of living cells imaging. Here, we review the recent developments of SIM and its combination with other imaging techniques.

scholarly journals Cell‐Permeable Nanobodies Allow Dual‐Color Super‐Resolution Microscopy in Untransfected Living Cells

2021 ◽  
Author(s):  
Anselm Fabian Lowell Schneider ◽  
Laila Benz ◽  
Martin Lehmann ◽  
Christian Hackenberger
Keyword(s):  
Super Resolution ◽  
Living Cells ◽  
Dual Color ◽  
Super Resolution Microscopy
Sign in / Sign up

Export Citation Format

Share Document