Internal standard evaluation for bioimaging soybean leaves through laser ablation inductively coupled plasma mass spectrometry: a plant nanotechnology approach

2018 ◽  
Vol 33 (10) ◽  
pp. 1720-1728 ◽  
Author(s):  
Katherine Chacón-Madrid ◽  
Marco Aurélio Zezzi Arruda
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Elemental Mapping ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Imaging Mode ◽  
Average Size ◽  
Soybean Leaves

This work evaluates the use of an internal standard for acquiring the elemental mapping of 107Ag, 55Mn and 63Cu using laser ablation inductively coupled plasma mass spectrometry in the imaging mode from soybean leaves cultivated in the absence or in the presence of silver nanoparticles (40 nm average size) and also using silver nitrate for comparative purposes.

Investigation of Light Scattering for Normalization of Signals in Laser Ablation Inductively Coupled Plasma Mass Spectrometry

1998 ◽  
Vol 52 (1) ◽  
pp. 154-160 ◽  
Author(s):  
S. A. Baker ◽  
B. W. Smith ◽  
J. D. Winefordner
Keyword(s):  
Mass Spectrometry ◽  
Light Scattering ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Particle Sizes ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Scattering Signal ◽  
Ablated Mass

Light scattering is evaluated for normalizing signals in laser ablation inductively coupled plasma mass spectrometry. The scattering signal produced from the transported ablation aerosol is measured with a laboratory-constructed cell. The technique is used to account for variations in the ablated mass within a matrix as well as between matrices. Matrices that are studied include brass, glass, soil, and Macor® ceramic. It is demonstrated that the technique is useful for normalizing analyte signals within a matrix; however, it is not as effective as the use of an internal standard in terms of the precision obtained. The utility of the technique to normalize between matrices is studied for glass, Macor®, and soil. The results indicate that light scattering is useful provided that the particle sizes generated are sufficiently similar.

Application of laser ablation (imaging) inductively coupled plasma mass spectrometry for mapping and quantifying Fe in transgenic and non-transgenic soybean leaves

2015 ◽  
Vol 30 (2) ◽  
pp. 389-395 ◽  
Author(s):  
Silvana R. Oliveira ◽  
Marco A. Z. Arruda
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Growth Chamber ◽  
Transgenic Soybean ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Soybean Leaves

Laser ablation inductively coupled plasma mass spectrometry is used for determining Fe in leaves of transgenic (variety M 7211RR) and non-transgenic (variety MSOY 8200) soybean, grown for 21 days in a growth chamber at controlled temperature (27 ± 0.1 °C) and photoperiod (12 h).

Influence of Different Metal Matrices on Manganese Signal Response in Laser Ablation Inductively Coupled Plasma—Mass Spectrometry

2009 ◽  
Vol 63 (8) ◽  
pp. 859-864 ◽  
Author(s):  
R. Usero ◽  
A. G. Coedo ◽  
M. T. Dorado ◽  
I. Padilla
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Signal Intensity ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Manganese Concentration ◽  
Inductively Coupled ◽  
Relative Standard ◽  
Plasma Mass Spectrometry ◽  
Signal Response

Laser ablation inductively coupled plasma–mass spectrometry (LA-ICP-MS) is applied for the quantitative determination of manganese concentration in metal samples. A set of Certified Reference Materials, with different matrix elements (Fe, Cu, Ni, Co, Al) and with certified Mn values, were analyzed. Absorption and plasma break-down depend on the solid phase; as a result, there are differences in mass ablation rates that influence the Mn signal response. In order to be able to compare Mn signals, the relative element-dependent response of tested metal matrices was determined from the ablation of Fe, Cu, Ni, Co, and Al pure metal targets. Relative sensitivity factors (RSFs), calculated as the ratio of the signal intensity of 59Co, 58Ni, 63Cu, and 27Al to the signal intensity of 56Fe, were: 0.79, 0.71, 0.74, and 0.51, respectively. All the isotope signals were matched to the value that should correspond to the relative abundance of 56Fe (91.75%). These experimentally determined RSFs were applied for compensating the observed differences. For quantitative measurements a set of CRM carbon steel samples (SS-451/1 to SS-460/1), with Fe contents of approximately 98%, was used for calibration. The samples with different matrices were measured using the main matrix element as internal standard; for this purpose, the measured intensity was firstly extrapolated, from their content in the sample, to the value that should correspond to a content of 98% and, after, was corrected with the calculated RSF. The developed quantification approach provides manganese results with deviations of 5–10% from the certified values, with relative standard deviations of 3–8%.

Study of Solution Calibration of NIST Soil and Glass Samples by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

2000 ◽  
Vol 54 (5) ◽  
pp. 639-644 ◽  
Author(s):  
Melody Bi ◽  
M. Antonio Ruiz ◽  
Benjamin W. Smith ◽  
James D. Winefordner
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Trace Element ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Time Resolved ◽  
Inductively Coupled ◽  
Element Concentrations ◽  
The Matrix ◽  
Plasma Mass Spectrometry

A method for the determination of trace element concentrations by laser ablation inductively coupled plasma mass spectrometry (ICP-MS) using solution calibration and an internal standard has been studied and evaluated by analyzing NIST soil and glass samples. In most cases, the measured element concentrations were within ±10% of the certified values. The internal standard was chosen on the basis of investigations of the proper signal intensity of certain isotopes and the homogeneity of their distribution in the sample. For soil samples, a matrix element, Mg, was chosen to be the internal standard. For glass samples, a trace element, Sr, was used as the internal standard. The results indicated that in both cases the internal standard was effective. Ni and Cu in soil gave poor results, while good results were obtained for Ni and Cu in glass samples. Time-resolved studies show that Ni gave many more signal spikes than other elements when the ablating laser moved across the surface of the soil sample. This observation indicates that one possible reason for the poor results was caused by the heterogeneity of the Ni distribution in the matrix.

Elemental mapping in fossil tooth root section of Ursus arctos by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)

Talanta ◽  
2013 ◽  
Vol 105 ◽  
pp. 235-243 ◽  
Author(s):  
M. Vašinová Galiová ◽  
M. Nývltová Fišáková ◽  
J. Kynický ◽  
L. Prokeš ◽  
H. Neff ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Ursus Arctos ◽  
Tooth Root ◽  
Elemental Mapping ◽  
Root Section ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

scholarly journals The individual analysis of fluid inclusions in minerals using Laser Ablation Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS): an evaluation

2001 ◽  
Vol 3 (3-4) ◽  
pp. 215-230
Author(s):  
Wouter HEIJLEN ◽  
Philippe MUCHEZ
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Fluid Inclusions ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Natural Quartz ◽  
Inductively Coupled ◽  
Ms Analysis ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

During the last decade, the possible application of laser ablation inductively coupled plasma - mass spectrometry (LA-ICP-MS) as a quantitative technique for the analysis of individual fluid inclusions has been intensely studied. The quantitative ability of this technique is, however, complicated by several fractionation processes that operate during ablation, transport and analysis in the ICP-MS. In the present study, a number of these fractionation effects were studied and the quantitative ability of LA-ICP-MS analysis of fluid inclusions in natural quartz is evaluated. Using NIST SRM 612 and 614 as reference materials, it is shown that the fractionation during transport is minimised when the sample cell is flushed with He, in contrast with the set-up where Ar is used. Calcium has been successfully applied as an internal standard to calibrate the REE in NIST-glasses. The use of Ca to calibrate other lithophile and chalcophile elements, such as K, Zn, Cu and Pb, can however be questioned. It is shown that the technique is capable of semi-quantitatively characterising different fluid inclusion populations in natural quartz, which demonstrates its importance as a tool for palaeofluidflow modelling. However, during LA-ICP-MS analysis of fluid inclusions in natural quartz, elements are reprecipitated in a glassy phase, as shown by SEM-EDX analysis of the sample surface after ablation. This process could result in a fractionation and may account for the poor precision of the analysis.

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