scholarly journals Multiphoton fluorescence lifetime imaging microscopy (FLIM) and super-resolution fluorescence imaging with a supramolecular biopolymer for the controlled tagging of polysaccharides

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9498-9507 ◽  
Author(s):  
Haobo Ge ◽  
Fernando Cortezon-Tamarit ◽  
Hui-Chen Wang ◽  
Adam C. Sedgwick ◽  
Rory L. Arrowsmith ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Lifetime ◽  
Super Resolution ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Boronate Ester ◽  
Lifetime Imaging ◽  
In Cells

A new coumarin-appended boronate ester for fluorogenic imaging which binds polysaccharides in solution and in cells.

Quantitative pH Imaging in Cells Using Confocal Fluorescence Lifetime Imaging Microscopy

1995 ◽  
Vol 227 (2) ◽  
pp. 302-308 ◽  
Author(s):  
R. Sanders ◽  
A. Draaijer ◽  
H.C. Gerritsen ◽  
P.M. Houpt ◽  
Y.K. Levine
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Ph Imaging ◽  
Confocal Fluorescence ◽  
In Cells

scholarly journals Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Chao Liu ◽  
Xinwei Wang ◽  
Yan Zhou ◽  
Yuliang Liu
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
Fluorescence Lifetime ◽  
Imaging Techniques ◽  
Absolute Measurement ◽  
Operating Mode ◽  
Least Square ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity, fluorophore concentration, and photobleaching compared to fluorescence intensity imaging techniques. Time-gated fluorescence lifetime imaging microscopy (FLIM) can provide high resolution and high imaging frame during mature FLIM methods. An abstract time-gated FLIM model was given, and important temporal parameters are shown as well. Aiming at different applications of steady and transient fluorescence processes, two different operation modes, timing and lifetime computing algorithm are designed. High resolution and high frame can be achieved by one-excitation one-sampling mode and least square algorithm for steady imaging applications. Correspondingly, one-excitation two-sampling mode and rapid lifetime determination algorithm contribute to transient fluorescence situations.

scholarly journals Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopy

2003 ◽  
Vol 373 (3) ◽  
pp. 999-999
Author(s):  
V. CALLEJA ◽  
S. M. AMEER-BEG ◽  
B. VOJNOVIC ◽  
R. WOSCHOLSKI ◽  
J. DOWNWARD ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Cells

P1-214 Demonstration of BACE phosphorylation and its interaction with GGA1 in cells by fluorescence lifetime imaging microscopy (FLIM)

2004 ◽  
Vol 25 ◽  
pp. S157
Author(s):  
Christine A. von Arnim ◽  
Michelle M. Tangredi ◽  
Ithan D. Peltan ◽  
Tejal Shah ◽  
Bradley T. Hyman
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Cells
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