scholarly journals Chemically-defined lactose-based autoinduction medium for site-specific incorporation of non-canonical amino acids into proteins

RSC Advances ◽  
2018 ◽  
Vol 8 (45) ◽  
pp. 25558-25567 ◽  
Author(s):  
Michael Muzika ◽  
Natali H. Muskat ◽  
Shani Sarid ◽  
Oshrit Ben-David ◽  
Ryan A. Mehl ◽  
...  
Keyword(s):  
Amino Acids ◽  
Site Specific ◽  
Expression Levels ◽  
Autoinduction Medium

Optimized chemically-defined lactose-based autoinduction media for superior expression levels of proteins with non-canonical amino acids.

scholarly journals Correction to Efficient Site-Specific Prokaryotic and Eukaryotic Incorporation of Halotyrosine Amino Acids into Proteins

2020 ◽  
Author(s):  
Hyo Sang Jang ◽  
Xiaodong Gu ◽  
Richard B. Cooley ◽  
Joseph J. Porter ◽  
Rachel L. Henson ◽  
...  
Keyword(s):  
Amino Acids ◽  
Site Specific

The effect of expression levels on codon usage inPlasmodium falciparum

Parasitology ◽  
2004 ◽  
Vol 128 (3) ◽  
pp. 245-251 ◽  
Author(s):  
L. PEIXOTO ◽  
V. FERNÁNDEZ ◽  
H. MUSTO
Keyword(s):  
Amino Acids ◽  
Plasmodium Falciparum ◽  
Natural Selection ◽  
Codon Usage ◽  
Complete Sequence ◽  
Expression Data ◽  
Expression Levels ◽  
Synonymous Codons ◽  
Translational Selection ◽  
Highly Expressed Genes

The usage of alternative synonymous codons in the completely sequenced, extremely A+T-rich parasitePlasmodium falciparumwas studied. Confirming previous studies obtained with less than 3% of the total genes recently described, we found that A- and U-ending triplets predominate but translational selection increases the frequency of a subset of codons in highly expressed genes. However, some new results come from the analysis of the complete sequence. First, there is more variation in GC3 than previously described; second, the effect of natural selection acting at the level of translation has been analysed with real expression data at 4 different stages and third, we found that highly expressed proteins increment the frequency of energetically less expensive amino acids. The implications of these results are discussed.

ChemInform Abstract: Synthesis and Site-Specific Incorporation of Red-Shifted Azobenzene Amino Acids into Proteins.

ChemInform ◽  
2016 ◽  
Vol 47 (19) ◽  
Author(s):  
Alford A. John ◽  
Carlo P. Ramil ◽  
Yulin Tian ◽  
Gang Cheng ◽  
Qing Lin
Keyword(s):  
Amino Acids ◽  
Site Specific

Site-specific mutagenesis with unnatural amino acids

1989 ◽  
Vol 14 (10) ◽  
pp. 400-403 ◽  
Author(s):  
Spencer J. Anthony-Cahill ◽  
Michael C. Griffith ◽  
Christopher J. Noren ◽  
Daniel J. Suich ◽  
Peter G. Schultz
Keyword(s):  
Amino Acids ◽  
Unnatural Amino Acids ◽  
Site Specific

Stereoselective and Site-Specific Allylic Alkylation of Amino Acids and Small Peptides via a Pd/Cu Dual Catalysis

2017 ◽  
Vol 139 (29) ◽  
pp. 9819-9822 ◽  
Author(s):  
Xiaohong Huo ◽  
Rui He ◽  
Jingke Fu ◽  
Jiacheng Zhang ◽  
Guoqiang Yang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Allylic Alkylation ◽  
Small Peptides ◽  
Site Specific ◽  
Dual Catalysis
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