scholarly journals Purification and characterization of alkaline phosphatase from lactic acid bacteria

RSC Advances ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 354-360 ◽  
Author(s):  
Yu-Hao Chu ◽  
Xin-Xin Yu ◽  
Xing Jin ◽  
Yu-Tang Wang ◽  
Duo-Jia Zhao ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Organophosphorus Pesticides ◽  
Purification And Characterization

Alkaline phosphatase (ALP) excreted from lactic acid bacteria (LAB) showed the ability to degrade organophosphorus pesticides.

scholarly journals Purification and characterization of two bacteriocins produced by lactic acid bacteria isolated from Mongolian airag

2006 ◽  
Vol 101 (4) ◽  
pp. 837-848 ◽  
Author(s):  
B. Batdorj ◽  
M. Dalgalarrondo ◽  
Y. Choiset ◽  
J. Pedroche ◽  
F. Métro ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Purification And Characterization

scholarly journals Molecular Identification of Indigenous Lactic Acid Bacteria, Partial Purification and Characterization of β-Galactosidase Produced

2017 ◽  
Vol 3 (4) ◽  
pp. 247
Author(s):  
Tatik Khusniati ◽  
Sulistiania . ◽  
Bellen Nastitie Pamela Fury ◽  
Syamsul Falah ◽  
Abdul Choliq
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Ammonium Sulphate ◽  
Molecular Identification ◽  
Specific Activity ◽  
Partial Purification ◽  
Optimum Temperature ◽  
Purification And Characterization

<p>β-Galactosidase is enzyme which hydrolyze lactose to glucose and galactose, as lactose hydrolyzer. To know indigenous lactic acid bacteria (LAB) characteristics, indigenous LAB identification, partial purification and characterization of β-galactosidase produced was researched. LAB was molecularly identified, β-galactosidase partial purification was conducted by precipitation followed dialysis. β-Galactosidase  characterization was based on optimum activities of pH and temperature. The results show that LAB was identified as <em>Lactobacillus plantarum</em> B123. Optimum activity of precipited β-galactosidase was reached at 50 % ammonium sulphate. Activity and specific activity of 50 % precipited β-galactosidase were 95.675 U · mg<sup>–1</sup> and 32.268 U · mg<sup>–1</sup>, respectively. Precipited β-galactosidase resulted a purification level of 3.99 fold, a yield of 38.73 %, and a specific activity of 32.27 U · mg<sup>–1</sup> protein, while dialyzed β-galactosidase resulted 7.61 fold, 10.67 %, and 61.53 U· mg<sup>–1</sup> protein. Optimum temperature and pH for crude β-galactosidase were found at 55 °C and 7.0, respectively, while that dialyzed β-galactosidase were optimized at 50 °C and 7.0. Based on partial purification and characterization, <em>Lactobacillus plantarum</em> B123 is indigenous LAB which good for production of β-galactosidase.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong> characterization; dialysis; lactic acid bacteria; <em>Lactobacillus plantarum</em> B 123; β-galactosidase.</p></div>

scholarly journals Characterization of Bacteriocin Produced by Lactic Acid Bacteria Isolated from Solid Waste of Soymilk production

2021 ◽  
Vol 709 (1) ◽  
pp. 012020
Author(s):  
Evy Rossi ◽  
Akhyar Ali ◽  
Raswen Efendi ◽  
Fajar Restuhadi ◽  
Yelmira Zalfiatri ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Solid Waste

Screening of lactic acid bacteria for degrading organophosphorus pesticides and their potential protective effects against pesticide toxicity

LWT ◽  
2021 ◽  
pp. 111672
Author(s):  
Shaofeng Yuan ◽  
Changjian Li ◽  
Hang Yu ◽  
Yunfei Xie ◽  
Yahui Guo ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Organophosphorus Pesticides ◽  
Protective Effects ◽  
Pesticide Toxicity
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