Combined atomic force microscopy (AFM) and traction force microscopy (TFM) reveals a correlation between viscoelastic material properties and contractile prestress of living cells

Soft Matter ◽  
2019 ◽  
Vol 15 (8) ◽  
pp. 1721-1729 ◽  
Author(s):  
Nicolas Schierbaum ◽  
Johannes Rheinlaender ◽  
Tilman E. Schäffer
Keyword(s):  
Atomic Force Microscopy ◽  
Viscoelastic Material ◽  
Material Properties ◽  
Traction Force ◽  
Living Cells ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Atomic Force

Combined AFM with TFM is a powerful tool to simultaneously and directly measure “passive” viscoelastic material properties and “active” contractile prestress of living cells at the nanoscale.

scholarly journals Combining adhesive contact mechanics with a viscoelastic material model to probe local material properties by AFM

Soft Matter ◽  
2018 ◽  
Vol 14 (1) ◽  
pp. 140-150 ◽  
Author(s):  
Christian Ganser ◽  
Caterina Czibula ◽  
Daniel Tscharnuter ◽  
Thomas Schöberl ◽  
Christian Teichert ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Viscoelastic Material ◽  
Material Properties ◽  
Material Model ◽  
Adhesive Contact ◽  
Force Microscopy ◽  
Atomic Force ◽  
Local Material ◽  
Local Material Properties

We present an atomic force microscopy based method to study viscoelastic material properties at low indentation depths with non-negligible adhesion and surface roughness.

scholarly journals Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcos Penedo ◽  
Tetsuya Shirokawa ◽  
Mohammad Shahidul Alam ◽  
Keisuke Miyazawa ◽  
Takehiko Ichikawa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Membrane ◽  
Cell Biology ◽  
Cell Damage ◽  
Efficiency Analysis ◽  
Living Cells ◽  
Biomolecular Recognition ◽  
Cell Penetration ◽  
Force Microscopy ◽  
Atomic Force

AbstractOver the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.

scholarly journals Anisotropic Mechanical Properties of Living Cells Revealed by Integrated Spinning Disk Confocal and Atomic Force Microscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 513a
Author(s):  
Yuri M. Efremov ◽  
Mirian Velay-Lizancos ◽  
Daniel M. Suter ◽  
Pablo D. Zavattieri ◽  
Arvind Raman
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Spinning Disk ◽  
Anisotropic Mechanical Properties

scholarly journals 3P-311 Viscoelastic Properties of Many Living Cells Investigated by Atomic Force Microscopy(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S175
Author(s):  
Shinichiro Hiratsuka ◽  
Yusuke Mizutani ◽  
Masahiro Tsuchiya ◽  
Koichi Kawahara ◽  
Hiroshi Tokumoto ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Annual Meeting ◽  
Viscoelastic Properties ◽  
Living Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Biophysical Society

scholarly journals Continuous detection of extracellular ATP on living cells by using atomic force microscopy

1999 ◽  
Vol 96 (21) ◽  
pp. 12180-12185 ◽  
Author(s):  
S. W. Schneider ◽  
M. E. Egan ◽  
B. P. Jena ◽  
W. B. Guggino ◽  
H. Oberleithner ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cells ◽  
Extracellular Atp ◽  
Force Microscopy ◽  
Atomic Force
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