Detection of breast cancer-derived exosomes using the horseradish peroxidase-mimicking DNAzyme as an aptasensor

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Yu Zhou ◽  
Huiying Xu ◽  
Hua Wang ◽  
Bang-Ce Ye
Keyword(s):  
Breast Cancer ◽  
Horseradish Peroxidase ◽  
G Quadruplex ◽  
Muc1 Aptamer

A hairpin-like aptasensor combining the specific MUC1 aptamer with a hemin/G-quadruplex is developed for the detection of breast cancer exosomes.

Performance of a template enhanced hybridization process in biological media for the detection of a breast cancer biomarker

2020 ◽  
Vol 12 (6) ◽  
pp. 839-847
Author(s):  
Reyhaneh Tavakoli-Koopaei ◽  
Fatemeh Javadi-Zarnaghi ◽  
Maedeh Aghahosseini ◽  
Manoochehr Tavassoli ◽  
Mohammad Javad Rasaee
Keyword(s):  
Breast Cancer ◽  
Cancer Biomarker ◽  
Biological Media ◽  
G Quadruplex ◽  
Colorimetric Assays

A split G-quadruplex DNAzyme have been used for analysis of microRNA mimics in biological media with colorimetric assays based on Template enhanced Hybridization Processes, TeHyP.

scholarly journals Landscape of G-quadruplex DNA structural regions in breast cancer

2020 ◽  
Vol 52 (9) ◽  
pp. 878-883 ◽  
Author(s):  
Robert Hänsel-Hertsch ◽  
Angela Simeone ◽  
Abigail Shea ◽  
Winnie W. I. Hui ◽  
Katherine G. Zyner ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Quadruplex Dna ◽  
G Quadruplex

G‐Quadruplex‐Binding Small Molecule Induces Synthetic Lethality in Breast Cancer Cells by Inhibiting c‐MYC and BCL2 Expression

ChemBioChem ◽  
2020 ◽  
Vol 21 (7) ◽  
pp. 963-970 ◽  
Author(s):  
Rakesh Paul ◽  
Tania Das ◽  
Manish Debnath ◽  
Ajay Chauhan ◽  
Jyotirmayee Dash
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Small Molecule ◽  
Breast Cancer Cells ◽  
Synthetic Lethality ◽  
G Quadruplex ◽  
Bcl2 Expression

The biological activity of G-quadruplex DNA binding papaverine-derived ligand in breast cancer cells

2008 ◽  
Vol 27 (4) ◽  
pp. 289-296 ◽  
Author(s):  
Blazej Rubis ◽  
Mariusz Kaczmarek ◽  
Natalia Szymanowska ◽  
Elzbieta Galezowska ◽  
Andrzej Czyrski ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Biological Activity ◽  
Dna Binding ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Quadruplex Dna ◽  
G Quadruplex

scholarly journals Telomerase Inhibitor TMPyP4 Alters Adhesion and Migration of Breast-Cancer Cells MCF7 and MDA-MB-231

2019 ◽  
Vol 20 (11) ◽  
pp. 2670 ◽  
Author(s):  
Natalia Konieczna ◽  
Aleksandra Romaniuk-Drapała ◽  
Natalia Lisiak ◽  
Ewa Totoń ◽  
Anna Paszel-Jaworska ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Cell Survival ◽  
Cancer Cell ◽  
Anticancer Properties ◽  
Telomerase Inhibitor ◽  
G Quadruplex ◽  
Cancer Cell Survival ◽  
Cell Adhesion And Migration ◽  
And Migration

Human telomeres were one of the first discovered and characterized sequences forming quadruplex structures. Association of these structures with oncogenic and tumor suppressor proteins suggests their important role in cancer development and therapy efficacy. Since cationic porphyrin TMPyP4 is known as G-quadruplex stabilizer and telomerase inhibitor, the aim of the study was to analyze the anticancer properties of this compound in two different human breast-cancer MCF7 and MDA-MB-231 cell lines. The cytotoxicity of TMPyP4 alone or in combination with doxorubicin was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromid) and clonogenic assays, and the cell-cycle alterations were analyzed by flow cytometry. Telomerase expression and activity were evaluated using qPCR and telomeric repeat amplification protocol (TRAP) assays, respectively. The contribution of G-quadruplex inhibitor to protein pathways engaged in cell survival, DNA repair, adhesion, and migration was performed using immunodetection. Scratch assay and functional assessment of migration and cell adhesion were also performed. Consequently, it was revealed that in the short term, TMPyP4 neither revealed cytotoxic effect nor sensitized MCF7 and MDA-MB-231 to doxorubicin, but altered breast-cancer cell adhesion and migration. It suggests that TMPyP4 might substantially contribute to a significant decrease in cancer cell dissemination and, consequently, cancer cell survival reduction. Importantly, this effect might not be associated with telomeres or telomerase.

Cytochemical demonstration of estrogen binding sites in breast cancer by estradiol covalently linked to horseradish peroxidase

1985 ◽  
Vol 21 (5) ◽  
pp. 625-630 ◽  
Author(s):  
Sten-Erik Jansson ◽  
Johan Gripenberg ◽  
Sirkka-Liisa Karonen ◽  
Veli-Matti Wasenius ◽  
Pekka Pantzar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Horseradish Peroxidase ◽  
Binding Sites ◽  
Cytochemical Demonstration ◽  
Covalently Linked ◽  
Estrogen Binding
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