Synergistic use of electroosmotic flow and magnetic forces for nucleic acid extraction

The Analyst ◽  
2020 ◽  
Vol 145 (6) ◽  
pp. 2412-2419 ◽  
Author(s):  
Rachel N. Deraney ◽  
Lindsay Schneider ◽  
Anubhav Tripathi
Keyword(s):  
Nucleic Acid ◽  
Electric Field ◽  
Microfluidic Chip ◽  
Applied Electric Field ◽  
Electroosmotic Flow ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Magnetic Forces ◽  
Extraction And Purification

NA extraction and purification utilitzing a microfluidic chip with applied electric field to induce electroosmotic flow opposite the magnetic NA-bound bead mix.

scholarly journals A point of care platform based on microfluidic chip for nucleic acid extraction in less than 1 minute

2019 ◽  
Vol 13 (3) ◽  
pp. 034102 ◽  
Author(s):  
Jianzhong Zhang ◽  
Xiaosong Su ◽  
Jiasu Xu ◽  
Jin Wang ◽  
Juntian Zeng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Microfluidic Chip ◽  
Point Of Care ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals Automated TruTip nucleic acid extraction and purification from raw sputum

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199869 ◽  
Author(s):  
Nitu Thakore ◽  
Ryan Norville ◽  
Molly Franke ◽  
Roger Calderon ◽  
Leonid Lecca ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Extraction And Purification

scholarly journals A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3853-3861 ◽  
Author(s):  
Chenguang Zhang ◽  
Gongchen Sun ◽  
Satyajyoti Senapati ◽  
Hsueh-Chia Chang
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
High Yield ◽  
Acid Extraction ◽  
Field Flow Fractionation ◽  
Nucleic Acid Extraction ◽  
Isolation And Purification ◽  
Extraction And Purification ◽  
Flow Fractionation ◽  
Continuous Field

We report a new Bifurcated Continuous Field-Flow Fractionation (BCFFF) microfluidic chip for isolation and purification of nucleic acids from blood plasma with high and concentration-independent yield. The platform is ideal for isolation and quantification of small miRNAs.

scholarly journals Rapid Nucleic Acid Extraction and Purification Using a Miniature Ultrasonic Technique

Micromachines ◽  
2017 ◽  
Vol 8 (7) ◽  
pp. 228 ◽  
Author(s):  
Darren Branch ◽  
Erika Vreeland ◽  
Jamie McClain ◽  
Jaclyn Murton ◽  
Conrad James ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Extraction ◽  
Ultrasonic Technique ◽  
Nucleic Acid Extraction ◽  
Extraction And Purification

Solid phase nucleic acid extraction technique in a microfluidic chip using a novel non-chaotropic agent: dimethyl adipimidate

Lab on a Chip ◽  
2014 ◽  
Vol 14 (2) ◽  
pp. 359-368 ◽  
Author(s):  
Yong Shin ◽  
Agampodi Promoda Perera ◽  
Chee Chung Wong ◽  
Mi Kyoung Park
Keyword(s):  
Nucleic Acid ◽  
Microfluidic Chip ◽  
Solid Phase ◽  
Extraction Technique ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Chaotropic Agent

Semi-automatic instrumentation for nucleic acid extraction and purification to quantify pathogens on surfaces

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6586-6594 ◽  
Author(s):  
Won-Nyoung Lee ◽  
Hyun Jin Yoo ◽  
Kim Huyen Nguyen ◽  
Changyoon Baek ◽  
Junhong Min
Keyword(s):  
Nucleic Acid ◽  
Detection System ◽  
Automated Detection ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Extraction And Purification ◽  
Short Time

A semi-automated detection system compatible with PCR that can detect infectious pathogens on wide surfaces in a short time.

A fast and sensitive nucleic acid extraction method for the detection of Cryptosporidium by PCR in environmental water samples

2002 ◽  
Vol 2 (3) ◽  
pp. 95-100 ◽  
Author(s):  
J. Dellundé ◽  
S. Pina ◽  
J. Jofre ◽  
F. Lucena
Keyword(s):  
Nucleic Acid ◽  
Environmental Samples ◽  
Environmental Water ◽  
Acid Extraction ◽  
Nucleic Acid Binding ◽  
Nucleic Acid Extraction ◽  
Naturally Occurring ◽  
Extraction And Purification ◽  
Guanidinium Thiocyanate ◽  
Cryptosporidium Oocysts

A new protocol based on a combination of an excystation process followed by a nucleic acid extraction that combines the lysing and nuclease-inactivating properties of the chaotropic agent guanidinium thiocyanate and the nucleic acid-binding properties of silica particles is described for the extraction and purification of nucleic acids from Cryptosporidium. The application of nested and/or semi-nested PCR using different external and internal primers to DNA extracted by this method from seeded and naturally occurring Cryptosporidium oocysts concentrated and purified from environmental samples detects numbers of oocysts ranging from 20 to 50. The method is feasible, detects mostly excystable oocysts and no problems of inhibition of PCR were observed when applied to environmental samples.

scholarly journals A LAMP-based microfluidic chip for rapid detection of pathogen in Cryptococcal meningitis

2020 ◽  
Author(s):  
Yueru Tian ◽  
Tong Zhang ◽  
Jian Guo ◽  
Huijun Lu ◽  
Yuhan Yao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Microfluidic Chip ◽  
Cryptococcal Meningitis ◽  
Rapid Detection ◽  
High Efficiency ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Membrane Structures ◽  
Operation Process ◽  
Global Threat

AbstractCryptococcal meningitis (CM) is a global threat with significant attributable morbidity and mortality. Information on integrated detection for CM diagnosis is still limited. This is mainly due to the presence of a large polysaccharide capsule and the tough cell wall of Cryptococcus, which makes it difficult to extract nucleic acids on the chip. In this study, we developed a LAMP-based microfluidic chip for rapid detection of pathogen in CM. We adopted 4 duplicate filtration membrane structures to improve target capture and simplify the enrichment process, and combined lyticase digestion and thermal alkaline lysisto optimize the nucleic acid extraction of Cryptococcus on the chip, and selected a portable UVA flashlight to shine the LAMP products to obtain the visual detection results which could be observed by the naked eye. This microfluidic chip, integrating sample Cryptococcus enrichment, nucleic acid extraction and LAMP detection unit, streamlined the operation process and reduced the exposure risk of directly handling cryptococcal samples. It did not require any additional instruments and demonstrated a rapid, reliable, as well as high-efficiency approach. It truly realized the “sample-to-answer” application and could be easily used for clinical cryptococcal prediagnosis.

scholarly journals Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 188
Author(s):  
Tanja Hoffmann ◽  
Andreas Hahn ◽  
Jaco J. Verweij ◽  
Gérard Leboulle ◽  
Olfert Landt ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Pcr Assay ◽  
Bead Beating ◽  
Stool Samples ◽  
Pcr Assays ◽  
Human Stool

This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as well as proteinase K digestion were compared with group-, genus-, and species-specific real-time PCR assays targeting helminths and nonhelminth pathogens in human stool samples. From 25 different in-house and commercial helminth real-time PCR assays applied to 77 stool samples comprising 67 historic samples and 10 external quality assessment scheme samples positively tested for helminths, higher numbers of positive test results were observed after bead-beating-based nucleic acid extraction for 5/25 (20%) real-time PCR assays irrespective of specificity issues. Lower cycle threshold values were observed for one real-time PCR assay after the standard extraction scheme, and for four assays after the bead-beating-based scheme. Agreement between real-time PCR results after both nucleic acid extraction strategies according to Cohen’s kappa ranged from poor to almost perfect for the different assays. Varying agreement was observed in eight nonhelminth real-time PCR assays applied to 67 historic stool samples. The study indicates highly variable effects of harsh nucleic acid extraction approaches depending on the real-time PCR assay used.

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