Environment-dependent single-chain mechanics of synthetic polymers and biomacromolecules by atomic force microscopy-based single-molecule force spectroscopy and the implications for advanced polymer materials

2020 ◽  
Vol 49 (9) ◽  
pp. 2799-2827 ◽  
Author(s):  
Yu Bao ◽  
Zhonglong Luo ◽  
Shuxun Cui
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Rational Design ◽  
Force Spectroscopy ◽  
Synthetic Polymers ◽  
Polymer Materials ◽  
Single Chain ◽  
Single Molecule Force Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force

Environment-dependent single-chain mechanics of macromolecules by AFM-SMFS is essential to the bottom-up rational design of polymer materials.

Reverse Engineering of an Affinity-Switchable Molecular Interaction Characterized by Atomic Force Microscopy Single-Molecule Force Spectroscopy†

Langmuir ◽  
2008 ◽  
Vol 24 (4) ◽  
pp. 1365-1370 ◽  
Author(s):  
Dario Anselmetti ◽  
Frank Wilco Bartels ◽  
Anke Becker ◽  
Björn Decker ◽  
Rainer Eckel ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Reverse Engineering ◽  
Single Molecule ◽  
Molecular Interaction ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force

Going Vertical To Improve the Accuracy of Atomic Force Microscopy Based Single-Molecule Force Spectroscopy

ACS Nano ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 198-207 ◽  
Author(s):  
Robert Walder ◽  
William J. Van Patten ◽  
Ayush Adhikari ◽  
Thomas T. Perkins
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force

Single-Molecule Force Spectroscopy onBombyx moriSilk Fibroin by Atomic Force Microscopy

Langmuir ◽  
2000 ◽  
Vol 16 (9) ◽  
pp. 4305-4308 ◽  
Author(s):  
Wenke Zhang ◽  
Qiaobing Xu ◽  
Shan Zou ◽  
Hongbin Li ◽  
Weiqing Xu ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Bioconjugation Strategies for Connecting Proteins to DNA-Linkers for Single-Molecule Force-Based Experiments

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2424
Author(s):  
Lyan M. van der Sleen ◽  
Katarzyna M. Tych
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Optical Tweezers ◽  
Single Molecule ◽  
Short Range ◽  
Force Spectroscopy ◽  
Magnetic Tweezers ◽  
Single Molecule Force Spectroscopy ◽  
Force Microscopy ◽  
Atomic Force

The mechanical properties of proteins can be studied with single molecule force spectroscopy (SMFS) using optical tweezers, atomic force microscopy and magnetic tweezers. It is common to utilize a flexible linker between the protein and trapped probe to exclude short-range interactions in SMFS experiments. One of the most prevalent linkers is DNA due to its well-defined properties, although attachment strategies between the DNA linker and protein or probe may vary. We will therefore provide a general overview of the currently existing non-covalent and covalent bioconjugation strategies to site-specifically conjugate DNA-linkers to the protein of interest. In the search for a standardized conjugation strategy, considerations include their mechanical properties in the context of SMFS, feasibility of site-directed labeling, labeling efficiency, and costs.

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