Identification of a new benzophenone from Psidium guajava L. leaves and its antineoplastic effects on human colon cancer cells

Oyaksungisan (OY) is a traditional herbal formula broadly used to treat beriberi, vomiting, diarrhea, and circulatory disturbance in Asian countries from ancient times. The effect of OY on cancer, however, was not reported until now. In this study, we have demonstrated that OY inhibits cell proliferation and induces cell deathviamodulating the autophagy on human colon cancer cells. In HCT116 cells, OY increased the ratio of LC3-II/LC3-I, a marker of autophagy, and treatment with 3-MA, an inhibitor of autophagy, and considerably reduced the formation of autophagosomes. In addition, OY regulated mitogen-activated protein kinase (MAPK) cascades; especially, JNK activation was closely related with autophagy effect by OY in HCT116 cells. Our results indicate that autophagy induction is responsible for the antiproliferative effect by OY, despite the weak apoptosis induction in HCT116 cells. In conclusion, OY might have a potential to be developed as an herbal anticancer remedy.

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New insights into the molecular mechanism of Boletus edulis ribonucleic acid fraction (BE3) concerning antiproliferative activity on human colon cancer cells

Food & Function ◽

10.1039/c6fo01626j ◽

2017 ◽

Vol 8 (5) ◽

pp. 1830-1839 ◽

Cited By ~ 4

Author(s):

Marta Kinga Lemieszek ◽

Miguel Ribeiro ◽

Guilhermina Marques ◽

Fernando Milheiro Nunes ◽

Piotr Pożarowski ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Molecular Mechanism ◽

Antiproliferative Activity ◽

Ribonucleic Acid ◽

Human Colon ◽

Acid Fraction ◽

Colon Cancer Cells ◽

Human Colon Cancer ◽

Human Colon Cancer Cells

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Abstract 1276: Application of high-throughput proteomics Micro-Western array in studying molecular mechanism of anticancer effect of liver X receptor agonists against human colon cancer cells

10.1158/1538-7445.am2012-1276 ◽

2012 ◽

Author(s):

Ching-Yu Lin ◽

Chiech Huo ◽

Hui-Ping Lin ◽

Shih Sheng Jiang ◽

Chih-Pin Chuu

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Molecular Mechanism ◽

High Throughput ◽

Human Colon ◽

Liver X Receptor ◽

Colon Cancer Cells ◽

Anticancer Effect ◽

Human Colon Cancer ◽

Human Colon Cancer Cells

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Zedoary Turmeric Oil Induces Senescence and Apoptosis in Human Colon Cancer HCT116 Cells

Natural Product Communications ◽

10.1177/1934578x1801300731 ◽

2018 ◽

Vol 13 (7) ◽

pp. 1934578X1801300

Author(s):

Meng-Qi Su ◽

Yi-Ran Zhou ◽

Cheng-Qin Li ◽

Zhou Wang ◽

Yue-Liang Wang ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Western Blotting ◽

Human Colon ◽

Dependent Manner ◽

Colon Cancer Cells ◽

Human Colon Cancer ◽

Related Proteins ◽

Human Colon Cancer Cells ◽

Hct116 Cells

Zedoary turmeric oil (ZTO) is a volatile oil that is extracted from the dry rhizome of Curcuma zedoaria with a variety of biological activities, including anti-tumor activity. However, there is a lack of knowledge about the effect and mechanism of ZTO in human colon cancer cells. The aim of this study was to examine the potential efficacy of ZTO against human colon cancer cells (HCT116) and to uncover the molecular mechanisms of its anti-tumor effects. The anti-proliferative activity of ZTO was determined by the MTT assay, cell counts and colony formation assay. Senescent cells were detected using SA-β-Gal staining, while apoptosis and the CD44+ subpopulation were evaluated by flow cytometry. The expression levels of senescence- and apoptosis-related proteins were examined using western blotting. The results showed that treatment with ZTO significantly inhibited the growth of HCT116 cells and caused senescence and apoptosis in a dose- and time-dependent manner. Western blotting revealed that ZTO significantly increased the expression of senescence- and apoptosis-related proteins p16, p21, and p53 and the phosphorylation of ERK. Moreover, ZTO treatment reduced the cancer stem-like CD44 positive cell population. These findings suggest that ZTO inhibits human colon cancer cells by inducing senescence and apoptosis.

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