An electrochemical immunosensor based on a combined amplification strategy with the GO–CS/CeO2–CS nanocomposite for the detection of aflatoxin M1

2020 ◽  
Vol 44 (4) ◽  
pp. 1362-1370 ◽  
Author(s):  
Xingshuang An ◽  
Xiaojie Shi ◽  
Hui Zhang ◽  
Yao Yao ◽  
Guangxian Wang ◽  
...  

Herein, a sensitive electrochemical immunosensor modified with graphene oxide–chitosan (GO–CS) and cerium oxide–chitosan (CeO2–CS) using screen-printed electrodes (SPEs) was developed for the determination of aflatoxin M1 (AFM1) in milk.

2005 ◽  
Vol 21 (4) ◽  
pp. 588-596 ◽  
Author(s):  
L. Micheli ◽  
R. Grecco ◽  
M. Badea ◽  
D. Moscone ◽  
G. Palleschi

2018 ◽  
Vol 31 (1) ◽  
pp. 153-159 ◽  
Author(s):  
Raquel G. Rocha ◽  
Jéssica S. Stefano ◽  
Iana V. S. Arantes ◽  
Michelle M. A. C. Ribeiro ◽  
Mario H. P. Santana ◽  
...  

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4324 ◽  
Author(s):  
Nurul Talib ◽  
Faridah Salam ◽  
Yusran Sulaiman

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).


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