scholarly journals Discovery of potential therapeutic targets for non-small cell lung cancer using high-throughput metabolomics analysis based on liquid chromatography coupled with tandem mass spectrometry

RSC Advances ◽  
2019 ◽  
Vol 9 (19) ◽  
pp. 10905-10913 ◽  
Author(s):  
Hong-dan Xu ◽  
Wen Luo ◽  
Yuanlong Lin ◽  
Jiawen Zhang ◽  
Lijuan Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
High Throughput ◽  
Cell Lung Cancer ◽  
Therapeutic Targets ◽  
Small Cell ◽  
Tandem Mass ◽  
Small Cell Lung ◽  
Metabolomics Analysis

High-throughput metabolomics can discover potential therapeutic targets for non-small cell lung cancer.

Chemokines as Therapeutic Targets in Non-Small Cell Lung Cancer

2002 ◽  
Vol 2 (3) ◽  
pp. 403-417 ◽  
Author(s):  
Bentham Science Publisher E.S. White ◽  
Bentham Science Publisher R.M. Strieter ◽  
Bentham Science Publisher D.A. Arenberg
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Therapeutic Targets ◽  
Small Cell ◽  
Small Cell Lung

scholarly journals A novel 96-microwell-based high-throughput spectrophotometric assay for pharmaceutical quality control of crizotinib, a novel potent drug for the treatment of non-small cell lung cancer

2015 ◽  
Vol 51 (2) ◽  
pp. 439-447
Author(s):  
Tanveer Ahmed Wani ◽  
Ibrahim Ali Darwish
Keyword(s):  
Lung Cancer ◽  
Quality Control ◽  
Small Cell Lung Cancer ◽  
High Throughput ◽  
Cell Lung Cancer ◽  
Spectrophotometric Assay ◽  
Small Cell ◽  
Small Cell Lung ◽  
Pharmaceutical Quality ◽  
Colored Product

<p>This study describes the development and validation of a novel 96-microwell-based high throughput spectrophotometric assay for pharmaceutical quality control of crizotinib (CZT), a novel drug for the treatment of non-small cell lung cancer. We examined the reaction between CZT and 1,2-naphthoquinone-4-sulphonate, a chromogenic reagent. A red-colored product showing a maximum absorption peak (λ<sub>max</sub>) at 490 nm was produced in an alkaline medium (pH 9). We examined stoichiometry of the reaction and postulated the reaction mechanism. To our knowledge, this is the first study to describe a color-developing reaction for the proposed assay. The reaction was performed in a 96-microwell plate, and the absorbance of the colored product was measured using an absorbance reader at 490 nm. Under optimized reaction conditions, Beer's law, which shows a correlation between absorbance and CZT concentration, was obeyed in the range of 4-50 µg/well with an appropriate correlation coefficient (0.999). The limits of detection and quantification were 1.73 and 5.23 µg/well, respectively. The assay showed high precision and accuracy. The proposed assay was applied successfully for the determination of CZT in capsules. Thus, the assay proposed in this study is practical and valuable for routine application in pharmaceutical quality control laboratories.</p>

Further molecular profiling of tumors harboring therapeutic targets within non-small cell lung cancer.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. 8087-8087
Author(s):  
Solange Peters ◽  
Andreas Voss ◽  
Kenneth Russell ◽  
Sherri Z. Millis ◽  
Elisabeth Brambilla
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Therapeutic Targets ◽  
Molecular Profiling ◽  
Small Cell ◽  
Small Cell Lung
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