Structural characterization of Mesobuthus martensii Karsch peptides and anti-inflammatory potency evaluation in human vascular endothelial cells

Studies have reported that scorpion toxins have excellent anti-cancer effects; however, the anti-inflammatory activity of scorpion peptides has rarely been studied.

Download Full-text

A8404 Anti-inflammatory activity of secreted APE1/Ref-1 in vascular endothelial cells

Journal of Hypertension ◽

10.1097/01.hjh.0000548192.83951.12 ◽

2018 ◽

Vol 36 ◽

pp. e50

Author(s):

Hee Kyoung Joo ◽

Yu Ran Lee ◽

Eun Ok Lee ◽

Myoung Soo Park ◽

Sunga Choi ◽

...

Keyword(s):

Endothelial Cells ◽

Vascular Endothelial Cells ◽

Inflammatory Activity ◽

Vascular Endothelial ◽

Anti Inflammatory

Download Full-text

Anti-inflammatory and Antioxidant Effects of Pluchea indica Leaf Extract in TNF-α-Induced Human Endothelial Cells

Walailak Journal of Science and Technology (WJST) ◽

10.48048/wjst.2021.10271 ◽

2021 ◽

Vol 18 (10) ◽

Author(s):

Klaokwan SRISOOK ◽

Suthasinee JINDA ◽

Ekaruth SRISOOK

Keyword(s):

Endothelial Cells ◽

Cell Adhesion ◽

Leaf Extract ◽

Vascular Endothelial Cells ◽

Inflammatory Activity ◽

Dependent Manner ◽

Vascular Endothelial ◽

Herbal Tea ◽

Tnf Α ◽

Anti Inflammatory

Pluchea indica is a shrub plant found in mangrove forests. The leaves are consumed as food and herbal tea and exhibit various biological effects, such as antioxidant and anti-inflammatory activities, in macrophages and animal models. However, the inhibitory activity of P. indica leaf extract on vascular inflammation remains unknown. Therefore, this study investigated the effect of an ethanol extract from P. indica herbal tea leaves (PIE) on tumor necrosis factor-α (TNF-α)-induced human vascular endothelial EA.hy926 cells. The cytotoxic effect of PIE was determined by thiazolyl blue tetrazolium bromide assays. PIE at concentrations of 12.5 - 50 µg/mL did not show significant cytotoxicity, whereas PIE at concentrations ≥ 100 µg/mL decreased cell viability. PIE inhibited the production of reactive oxygen species (ROS) in TNF-α-stimulated endothelial cells. To evaluate the PIE’s anti-vascular inflammatory activity, the protein expression of cell adhesion molecules, including intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), was determined by western blot. PIE significantly decreased TNF-α-induced ICAM-1 and VCAM-1 expression in a concentration-dependent manner. Furthermore, PIE upregulated heme oxygenase-1 (HO-1) in a concentration- and time-dependent manner. Inhibiting the activity of HO-1 by tin protoporphyrin IX significantly blocked the suppressive effect of PIE on ICAM-1 but not VCAM-1 expression. Therefore, PIE exerts anti-inflammatory activity on vascular endothelial cells, at least in part, by suppressing ROS production and the induction of HO-1. The obtained data suggest that PIE is a promising substance for developing therapeutic agents or as an ingredient of functional food. HIGHLIGHTS Pluchea indica leaf extract (PIE) at non-toxic doses inhibited ICAM-1 and VCAM-1 in TNF-α-induced human vascular endothelial cells PIE suppressed the production of ROS in TNF-α-stimulated endothelial cells PIE exerts anti-inflammatory activity on vascular endothelial cells mediated partly through the upregulation of HO-1

Download Full-text

Abstract 153: Bile Acid Receptor TGR5 Agonism Represents Anti-inflammatory Effects via Stimulating Nitric Oxide Production in Vascular Endothelial Cells

Arteriosclerosis Thrombosis and Vascular Biology ◽

10.1161/atvb.32.suppl_1.a153 ◽

2012 ◽

Vol 32 (suppl_1) ◽

Author(s):

Taiki Kida ◽

Yoshiki Tsubosaka ◽

Masatoshi Hori ◽

Hiroshi Ozaki ◽

Takahisa Murata

Keyword(s):

Nitric Oxide ◽

Endothelial Cells ◽

Bile Acids ◽

Inflammatory Responses ◽

Vascular Endothelial Cells ◽

Lithocholic Acid ◽

Signal Pathways ◽

No Production ◽

Vascular Endothelial ◽

Anti Inflammatory

Objective TGR5, a membrane-bound, G-protein-coupled receptor for bile acids, is known to be involved in regulation of energy homeostasis and inflammation. However, little is known about the function of TGR5 in vascular endothelial cells. In the present study, we examined whether TGR5 agonism represents anti-inflammatory effects in vascular endothelial cells focusing on nitric oxide (NO) production. Methods and Results In human umbilical vein endothelial cells (HUVECs), treatment with taurolithocholic acid (TLCA), which has the highest affinity to TGR5 among various bile acids, significantly reduced tumor necrosis factor (TNF)-α-induced vascular cell adhesion molecule (VCAM)-1 protein expression and adhesion of human monocytes, U937. These effects were abrogated by a NO synthase (NOS) inhibitor, N G -Monomethyl-L-arginine (L-NMMA). In bovine aortic endothelial cells (BAECs), treatment with TLCA as well as lithocholic acid, which also has high affinity to TGR5, significantly increased the NO production. In contrast, deoxycholic acid and chenodeoxycholic acid, which possess low affinity to TGR5, did not affect the NO production. Gene depletion of TGR5 by siRNA transfection abolished TLCA-induced NO production in BAECs. TLCA-induced NO production was also observed in HUVECs measured as intracellular cGMP accumulation. We next investigated the signal pathways responsible for the TLCA-induced NO production in endothelial cells. Treatment with TLCA increased endothelial NOS (eNOS) ser1177 phosphorylation in HUVECs. This response was accompanied by increased Akt ser473 phosphorylation and intracellular Ca 2+ ([Ca 2+ ] i ). Treatment with phosphoinositide 3-kinase (PI3K) inhibitor, LY294002, or blockade of calcium channel with La 3+ , significantly decreased TLCA-induced eNOS ser1177 phosphorylation and subsequent NO production. Conclusion These results indicate that TGR5 agonism can mediate anti-inflammatory responses by suppressing VCAM-1 expression and monocytes adhesion to endothelial cells. This function is dependent on NO production via Akt activation and [Ca 2+ ] i increase.

Download Full-text