scholarly journals TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells

RSC Advances ◽  
2019 ◽  
Vol 9 (43) ◽  
pp. 25039-25047 ◽  
Author(s):  
Dhanya T. Jayaram ◽  
Ashwath Kumar ◽  
Linda E. Kippner ◽  
Po-Yi Ho ◽  
Melissa L. Kemp ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fluorescence Microscopy ◽  
Tio2 Nanoparticles ◽  
Human Lung ◽  
Lung Cells ◽  
Rna Seq ◽  
Nanoparticle Exposure ◽  
Human Lung Cells

Human lung cells have a multi-generational response to TiO2 nanoparticle exposure determined by RNA-Seq and fluorescence microscopy.

scholarly journals Gene expression profiles of normal human lung cells affected by adenoviral E1B

Virology ◽  
2006 ◽  
Vol 350 (2) ◽  
pp. 418-428 ◽  
Author(s):  
Xiao-Mei Rao ◽  
Xinyu Zheng ◽  
Sabine Waigel ◽  
Wolfgang Zacharias ◽  
Kelly M. McMasters ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Lung ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Normal Human

scholarly journals Influence of the physicochemical features of TiO2 nanoparticles on the formation of a protein corona and impact on cytotoxicity

RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 43950-43959
Author(s):  
Ozge Kose ◽  
Marion Stalet ◽  
Lara Leclerc ◽  
Valérie Forest
Keyword(s):  
Tio2 Nanoparticles ◽  
Human Lung ◽  
Protein Corona ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Potential Relationship ◽  
Physicochemical Features

Characterization of the formation of the protein corona of TiO2 nanoparticles as a function of the main nanoparticle properties and investigation of potential relationship with the cytotoxicity nanoparticles induce in vitro in human lung cells.

Gene Expression Modulation in A549 Human Lung Cells in Response to Combustion-Generated Nano-Sized Particles

2006 ◽  
Vol 1091 (1) ◽  
pp. 170-183 ◽  
Author(s):  
ANDREA ARENZ ◽  
CHRISTINE E. HELLWEG ◽  
NEVENA STOJICIC ◽  
CHRISTA BAUMSTARK-KHAN ◽  
HORST-HENNING GROTHEER
Keyword(s):  
Gene Expression ◽  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Expression Modulation

scholarly journals In vitro exposure to isoprene-derived secondary organic aerosol by direct deposition and its effects on <i>COX-2</i> and <i>IL-8</i> gene expression

2016 ◽  
Vol 16 (22) ◽  
pp. 14079-14090 ◽  
Author(s):  
Maiko Arashiro ◽  
Ying-Hsuan Lin ◽  
Kenneth G. Sexton ◽  
Zhenfa Zhang ◽  
Ilona Jaspers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Lung ◽  
Secondary Organic Aerosol ◽  
Organic Aerosol ◽  
Lung Cells ◽  
Mrna Levels ◽  
Altered Expression ◽  
Direct Deposition ◽  
Human Lung Cells ◽  
Cox 2

Abstract. Atmospheric oxidation of isoprene, the most abundant non-methane hydrocarbon emitted into Earth's atmosphere primarily from terrestrial vegetation, is now recognized as a major contributor to the global secondary organic aerosol (SOA) burden. Anthropogenic pollutants significantly enhance isoprene SOA formation through acid-catalyzed heterogeneous chemistry of epoxide products. Since isoprene SOA formation as a source of fine aerosol is a relatively recent discovery, research is lacking on evaluating its potential adverse effects on human health. The objective of this study was to examine the effect of isoprene-derived SOA on inflammation-associated gene expression in human lung cells using a direct deposition exposure method. We assessed altered expression of inflammation-related genes in human bronchial epithelial cells (BEAS-2B) exposed to isoprene-derived SOA generated in an outdoor chamber facility. Measurements of gene expression of known inflammatory biomarkers interleukin 8 (IL-8) and cyclooxygenase 2 (COX-2) in exposed cells, together with complementary chemical measurements, showed that a dose of 0.067 µg cm−2 of SOA from isoprene photooxidation leads to statistically significant increases in IL-8 and COX-2 mRNA levels. Resuspension exposures using aerosol filter extracts corroborated these findings, supporting the conclusion that isoprene-derived SOA constituents induce the observed changes in mRNA levels. The present study is an attempt to examine the early biological responses of isoprene SOA exposure in human lung cells.

scholarly journals Gene expression changes in human lung cells exposed to arsenic, chromium, nickel or vanadium indicate the first steps in cancer

Metallomics ◽  
2012 ◽  
Vol 4 (8) ◽  
pp. 784 ◽  
Author(s):  
Hailey A. Clancy ◽  
Hong Sun ◽  
Lisa Passantino ◽  
Thomas Kluz ◽  
Alexandra Muñoz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells

scholarly journals Global gene expression profiling in human lung cells exposed to cobalt

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 147 ◽  
Author(s):  
Veronique Malard ◽  
Frederic Berenguer ◽  
Odette Prat ◽  
Sylvie Ruat ◽  
Gerard Steinmetz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Human Lung ◽  
Global Gene Expression ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Global Gene Expression Profiling

scholarly journals Transcriptome Profiling and Toxicity Following Long-Term, Low Dose Exposure of Human Lung Cells to Ni and NiO Nanoparticles—Comparison with NiCl2

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 649 ◽  
Author(s):  
Anda R. Gliga ◽  
Sebastiano Di Bucchianico ◽  
Emma Åkerlund ◽  
Hanna L. Karlsson
Keyword(s):  
Gene Expression ◽  
Calcium Binding ◽  
Low Dose ◽  
Human Lung ◽  
Cell Transformation ◽  
Lung Cells ◽  
Nio Nanoparticles ◽  
Human Lung Cells

Production of nickel (Ni) and nickel oxide (NiO) nanoparticles (NPs) leads to a risk of exposure and subsequent health effects. Understanding the toxicological effects and underlying mechanisms using relevant in vitro methods is, therefore, needed. The aim of this study is to explore changes in gene expression using RNA sequencing following long term (six weeks) low dose (0.5 µg Ni/mL) exposure of human lung cells (BEAS-2B) to Ni and NiO NPs as well as soluble NiCl2. Genotoxicity and cell transformation as well as cellular dose of Ni are also analyzed. Exposure to NiCl2 resulted in the largest number of differentially expressed genes (197), despite limited uptake, suggesting a major role of extracellular receptors and downstream signaling. Gene expression changes for all Ni exposures included genes coding for calcium-binding proteins (S100A14 and S100A2) as well as TIMP3, CCND2, EPCAM, IL4R and DDIT4. Several top enriched pathways for NiCl2 were defined by upregulation of, e.g., interleukin-1A and -1B, as well as Vascular Endothelial Growth Factor A (VEGFA). All Ni exposures caused DNA strand breaks (comet assay), whereas no induction of micronuclei was observed. Taken together, this study provides an insight into Ni-induced toxicity and mechanisms occurring at lower and more realistic exposure levels.

scholarly journals Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol

2017 ◽  
Vol 51 (14) ◽  
pp. 8166-8175 ◽  
Author(s):  
Ying-Hsuan Lin ◽  
Maiko Arashiro ◽  
Phillip W. Clapp ◽  
Tianqu Cui ◽  
Kenneth G. Sexton ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Human Lung ◽  
Secondary Organic Aerosol ◽  
Organic Aerosol ◽  
Lung Cells ◽  
Human Lung Cells
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