scholarly journals Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol

2017 ◽  
Vol 51 (14) ◽  
pp. 8166-8175 ◽  
Author(s):  
Ying-Hsuan Lin ◽  
Maiko Arashiro ◽  
Phillip W. Clapp ◽  
Tianqu Cui ◽  
Kenneth G. Sexton ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Human Lung ◽  
Secondary Organic Aerosol ◽  
Organic Aerosol ◽  
Lung Cells ◽  
Human Lung Cells

scholarly journals In vitro exposure to isoprene-derived secondary organic aerosol by direct deposition and its effects on <i>COX-2</i> and <i>IL-8</i> gene expression

2016 ◽  
Vol 16 (22) ◽  
pp. 14079-14090 ◽  
Author(s):  
Maiko Arashiro ◽  
Ying-Hsuan Lin ◽  
Kenneth G. Sexton ◽  
Zhenfa Zhang ◽  
Ilona Jaspers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Lung ◽  
Secondary Organic Aerosol ◽  
Organic Aerosol ◽  
Lung Cells ◽  
Mrna Levels ◽  
Altered Expression ◽  
Direct Deposition ◽  
Human Lung Cells ◽  
Cox 2

Abstract. Atmospheric oxidation of isoprene, the most abundant non-methane hydrocarbon emitted into Earth's atmosphere primarily from terrestrial vegetation, is now recognized as a major contributor to the global secondary organic aerosol (SOA) burden. Anthropogenic pollutants significantly enhance isoprene SOA formation through acid-catalyzed heterogeneous chemistry of epoxide products. Since isoprene SOA formation as a source of fine aerosol is a relatively recent discovery, research is lacking on evaluating its potential adverse effects on human health. The objective of this study was to examine the effect of isoprene-derived SOA on inflammation-associated gene expression in human lung cells using a direct deposition exposure method. We assessed altered expression of inflammation-related genes in human bronchial epithelial cells (BEAS-2B) exposed to isoprene-derived SOA generated in an outdoor chamber facility. Measurements of gene expression of known inflammatory biomarkers interleukin 8 (IL-8) and cyclooxygenase 2 (COX-2) in exposed cells, together with complementary chemical measurements, showed that a dose of 0.067 µg cm−2 of SOA from isoprene photooxidation leads to statistically significant increases in IL-8 and COX-2 mRNA levels. Resuspension exposures using aerosol filter extracts corroborated these findings, supporting the conclusion that isoprene-derived SOA constituents induce the observed changes in mRNA levels. The present study is an attempt to examine the early biological responses of isoprene SOA exposure in human lung cells.

scholarly journals Global gene expression profiling in human lung cells exposed to cobalt

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 147 ◽  
Author(s):  
Veronique Malard ◽  
Frederic Berenguer ◽  
Odette Prat ◽  
Sylvie Ruat ◽  
Gerard Steinmetz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Human Lung ◽  
Global Gene Expression ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Global Gene Expression Profiling

scholarly journals TiO2 nanoparticles generate superoxide and alter gene expression in human lung cells

RSC Advances ◽  
2019 ◽  
Vol 9 (43) ◽  
pp. 25039-25047 ◽  
Author(s):  
Dhanya T. Jayaram ◽  
Ashwath Kumar ◽  
Linda E. Kippner ◽  
Po-Yi Ho ◽  
Melissa L. Kemp ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fluorescence Microscopy ◽  
Tio2 Nanoparticles ◽  
Human Lung ◽  
Lung Cells ◽  
Rna Seq ◽  
Nanoparticle Exposure ◽  
Human Lung Cells

Human lung cells have a multi-generational response to TiO2 nanoparticle exposure determined by RNA-Seq and fluorescence microscopy.

scholarly journals Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells

2007 ◽  
Vol 6 (1) ◽  
pp. 75 ◽  
Author(s):  
Dong-Cheol Seo ◽  
Ji-Min Sung ◽  
Hee-Jung Cho ◽  
Hee Yi ◽  
Kun-Ho Seo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Lung Adenocarcinoma ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Human Lung ◽  
A549 Cells ◽  
Human Lung Adenocarcinoma ◽  
Lung Adenocarcinoma A549

Gene Expression Profiling of Human Lung Tissue from Smokers with Severe Emphysema

2004 ◽  
Vol 31 (6) ◽  
pp. 601-610 ◽  
Author(s):  
Avrum Spira ◽  
Jennifer Beane ◽  
Victor Pinto-Plata ◽  
Aran Kadar ◽  
Gang Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Lung Tissue ◽  
Expression Profiling ◽  
Human Lung ◽  
Human Lung Tissue ◽  
Severe Emphysema

scholarly journals Gene expression profiles of normal human lung cells affected by adenoviral E1B

Virology ◽  
2006 ◽  
Vol 350 (2) ◽  
pp. 418-428 ◽  
Author(s):  
Xiao-Mei Rao ◽  
Xinyu Zheng ◽  
Sabine Waigel ◽  
Wolfgang Zacharias ◽  
Kelly M. McMasters ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Lung ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Normal Human

scholarly journals <i>In Vitro</i> Exposure to Isoprene-Derived Secondary Organic Aerosol by Direct Deposition and its Effects on <i>COX-2</i> and <i>IL-8</i> Gene Expression

2016 ◽  
Author(s):  
Maiko Arashiro ◽  
Ying-Hsuan Lin ◽  
Kenneth G. Sexton ◽  
Zhenfa Zhang ◽  
Ilona Jaspers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Secondary Organic Aerosol ◽  
Organic Aerosol ◽  
Mrna Levels ◽  
Altered Expression ◽  
Direct Deposition ◽  
Discovery Research ◽  
Human Lung Cells ◽  
Cox 2

Abstract. Atmospheric oxidation of isoprene, the most abundant non-methane hydrocarbon emitted into Earth’s atmosphere primarily from terrestrial vegetation, is now recognized as a major contributor to the global secondary organic aerosol (SOA) burden. Anthropogenic pollutants significantly enhance isoprene SOA formation through acid-catalyzed heterogeneous chemistry of epoxide products. Since isoprene SOA formation as a source of fine aerosol is a relatively recent discovery, research is lacking on evaluating its potential adverse effects on human health. The objective of this study was to examine the effect of isoprene-derived SOA on inflammation-associated gene expression in human lung cells using a direct deposition exposure method. We assessed altered expression of inflammation-related genes in human bronchial epithelial cells (BEAS-2B) exposed to isoprene-derived SOA generated in an outdoor chamber facility. Measurements of gene expression of known inflammatory biomarkers interleukin 8 (IL-8) and cyclooxygenase 2 (COX-2) in exposed cells, together with complementary chemical measurements, showed that a dose of 0.067 µg cm−2 of SOA from isoprene photooxidation leads to statistically significant increases in IL-8 and COX-2 mRNA levels. Resuspension exposures using aerosol filter extracts corroborated these findings, supporting the conclusion that isoprene-derived SOA constituents induce the observed changes in mRNA levels. Future studies are needed to systematically examine the molecular mechanisms of toxicity.

scholarly journals Gene Expression Profiling in Human Lung Development: An Abundant Resource for Lung Adenocarcinoma Prognosis

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105639 ◽  
Author(s):  
Lin Feng ◽  
Jiamei Wang ◽  
Bangrong Cao ◽  
Yi Zhang ◽  
Bo Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lung Adenocarcinoma ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Lung Development ◽  
Human Lung

scholarly journals Global Gene Expression Profiling of Human Lung Epithelial Cells After Exposure to Nanosilver

2012 ◽  
Vol 130 (1) ◽  
pp. 145-157 ◽  
Author(s):  
Rasmus Foldbjerg ◽  
Eveline S. Irving ◽  
Yuya Hayashi ◽  
Duncan S. Sutherland ◽  
Kasper Thorsen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Human Lung ◽  
Global Gene Expression ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Global Gene Expression Profiling ◽  
Human Lung Epithelial Cells
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