Recent advances in stimuli-responsive in situ self-assembly of small molecule probes for in vivo imaging of enzymatic activity

2021 ◽  
Author(s):  
Yuqi Wang ◽  
Jianhui Weng ◽  
Xidan Wen ◽  
Yuxuan Hu ◽  
Deju Ye
Keyword(s):  
Enzymatic Activity ◽  
Small Molecule ◽  
Self Assembly ◽  
In Vivo Imaging ◽  
Molecular Probes ◽  
Stimuli Responsive ◽  
Small Molecule Probes ◽  
Recent Advances

Stimuli-responsive in situ self-assembly of small molecule probes into nanostructures has been promising for the construction of molecular probes for in vivo imaging.

scholarly journals Bioorthogonal cyclization-mediated in situ self-assembly of small-molecule probes for imaging caspase activity in vivo

2014 ◽  
Vol 6 (6) ◽  
pp. 519-526 ◽  
Author(s):  
Deju Ye ◽  
Adam J. Shuhendler ◽  
Lina Cui ◽  
Ling Tong ◽  
Sui Seng Tee ◽  
...  
Keyword(s):  
Small Molecule ◽  
Self Assembly ◽  
Caspase Activity ◽  
Small Molecule Probes

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

scholarly journals Development of In Situ Self-Assembly Nanoparticles to Encapsulate Lopinavir and Ritonavir for Long-Acting Subcutaneous Injection

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 904
Author(s):  
Irin Tanaudommongkon ◽  
Asama Tanaudommongkon ◽  
Xiaowei Dong
Keyword(s):  
Sustained Release ◽  
Trough Concentration ◽  
Self Assembly ◽  
Subcutaneous Injection ◽  
Drug Loading ◽  
Entrapment Efficiency ◽  
Long Acting

Most antiretroviral medications for human immunodeficiency virus treatment and prevention require high levels of patient adherence, such that medications need to be administered daily without missing doses. Here, a long-acting subcutaneous injection of lopinavir (LPV) in combination with ritonavir (RTV) using in situ self-assembly nanoparticles (ISNPs) was developed to potentially overcome adherence barriers. The ISNP approach can improve the pharmacokinetic profiles of the drugs. The ISNPs were characterized in terms of particle size, drug entrapment efficiency, drug loading, in vitro release study, and in vivo pharmacokinetic study. LPV/RTV ISNPs were 167.8 nm in size, with a polydispersity index of less than 0.35. The entrapment efficiency was over 98% for both LPV and RTV, with drug loadings of 25% LPV and 6.3% RTV. A slow release rate of LPV was observed at about 20% on day 5, followed by a sustained release beyond 14 days. RTV released faster than LPV in the first 5 days and slower than LPV thereafter. LPV trough concentration remained above 160 ng/mL and RTV trough concentration was above 50 ng/mL after 6 days with one subcutaneous injection. Overall, the ISNP-based LPV/RTV injection showed sustained release profiles in both in vitro and in vivo studies.

scholarly journals Two-photon small-molecule fluorescence-based agents for sensing, imaging, and therapy within biological systems

2021 ◽  
Vol 50 (2) ◽  
pp. 702-734
Author(s):  
Luling Wu ◽  
Jihong Liu ◽  
Ping Li ◽  
Bo Tang ◽  
Tony D. James
Keyword(s):  
Small Molecule ◽  
Biological Systems ◽  
Two Photon ◽  
Small Molecule Probes ◽  
Recent Advances ◽  
Design Construction

In this tutorial review, we will explore recent advances for the design, construction and application of two-photon excited fluorescence (TPEF)-based small-molecule probes.

Enzyme‐mediated in situ self‐assembly promotes in vivo bioorthogonal reaction for pretargeted multimodality imaging

2021 ◽  
Author(s):  
Yuxuan Hu ◽  
Junya Zhang ◽  
Yinxing Miao ◽  
Xidan Wen ◽  
Jian Wang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Multimodality Imaging ◽  
Bioorthogonal Reaction

Controlled self-assembly of small molecule probes and the related applications in bioanalysis

2016 ◽  
Vol 76 ◽  
pp. 38-53 ◽  
Author(s):  
Yongxin Li ◽  
Huipeng Zhou ◽  
Jian Chen ◽  
Sohail Anjum Shahzad ◽  
Cong Yu
Keyword(s):  
Small Molecule ◽  
Self Assembly ◽  
Small Molecule Probes

scholarly journals Aqueous interfacial gels assembled from small molecule supramolecular polymers

2017 ◽  
Vol 8 (2) ◽  
pp. 1350-1355 ◽  
Author(s):  
Alexander S. Groombridge ◽  
Aniello Palma ◽  
Richard M. Parker ◽  
Chris Abell ◽  
Oren A. Scherman
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Self Assembly ◽  
Hyperbranched Polymer ◽  
Soft Matter ◽  
Supramolecular Polymers ◽  
Supramolecular Network ◽  
Self Healing ◽  
Liquid Interfaces ◽  
Stimuli Responsive

The successful self-assembly of a stimuli-responsive aqueous supramolecular hyperbranched polymer from small molecules and the macrocyclic host cucurbit[8]uril (CB[8]) is reported. This self-healing supramolecular network can act as a soft matter barrier at liquid–liquid interfaces.

Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly

2019 ◽  
Vol 141 (26) ◽  
pp. 10331-10341 ◽  
Author(s):  
Runqi Yan ◽  
Yuxuan Hu ◽  
Fei Liu ◽  
Shixuan Wei ◽  
Daqing Fang ◽  
...  
Keyword(s):  
Self Assembly ◽  
In Vivo Imaging ◽  
Nir Fluorescence ◽  
Fluorogenic Reaction

scholarly journals Molecular probes for the in vivo imaging of cancer

2009 ◽  
Vol 5 (11) ◽  
pp. 1279 ◽  
Author(s):  
Raphael Alford ◽  
Mikako Ogawa ◽  
Peter L. Choyke ◽  
Hisataka Kobayashi
Keyword(s):  
In Vivo Imaging ◽  
Molecular Probes
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